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Gene Expression Data
Assay Details
Assay
Reference: J:157819 Shimogori T, et al., A genomic atlas of mouse hypothalamic development. Nat Neurosci. 2010;13(6):767-75
Assay type: RNA in situ
MGI Accession ID: MGI:4450814
Gene symbol: Foxb1
Gene name: forkhead box B1
Probe: IMAGE clone 40045942
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 4a: embryonic day 12.5 (more )
Note: Double labeled: blue - Foxb1; brown - Lhx6 (assay MGI:4452075).
Structure Level Pattern Image Note
TS20: diencephalon Present Regionally restricted 4a Expression is present in the mammillary nucleus.
Specimen 4b: embryonic day 16.5 (more )
Note: Double labeled: blue - Foxb1; brown - Lhx6 (assay MGI:4452075).
Structure Level Pattern Image Note
TS24: diencephalon Present Regionally restricted 4b Expression is present in the mammillary nucleus.
Specimen 6c: embryonic day 12.5 (more )
Note: Double labeled: blue - Foxb1; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS20: diencephalon Present Regionally restricted 6c Expression is present in the mammillary epithelium.
Specimen 6d: embryonic day 12.5; Shhtm2Amc/Shhtm2Amc, Tg(Nkx2-1-cre)2Sand/0 (more )
Note: Conditional mutant. Double labeled: blue - Foxb1; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS20: diencephalon Present Regionally restricted 6d Expression is present in the mammillary epithelium.
Specimen S4e: embryonic day 11.5 (more )
Note: Double labeled: blue - Foxb1; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted S4_e Expression is present in the mammillary nucleus.
Specimen S4f: embryonic day 12.5 (more )
Note: Double labeled: blue - Foxb1; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS20: diencephalon Present Regionally restricted S4_f Expression is present in the mammillary nucleus.
Specimen S4g: embryonic day 14.5 (more )
Note: Double labeled: blue - Foxb1; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS22: diencephalon Present Regionally restricted S4_g Expression is present in the mammillary nucleus.
Specimen S4h: embryonic day 16.5 (more )
Note: Double labeled: blue - Foxb1; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS24: diencephalon Present Regionally restricted S4_h Expression is present in the mammillary nucleus.
Specimen Foxb1 - E11.5: embryonic day 11.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS19: diencephalon lateral wall mantle layer Very strong Non-Uniform FOXB1(D)+SHH(F)-E11.5-coronal , FOXB1(D)+SHH(F)-E11.5-sagittal There is very strong staining in the MZ of the mam zone. Staining is either absent or ubiquitous in the VZ of the dorsal thalamus, prethalamus, dorsal & ventral POAs, PVH, VMH & ARH nuclei, the AVH, anterior & posterior IDs, premam & supramam zones & TT.
TS19: diencephalon lateral wall ventricular layer Ambiguous FOXB1(D)+SHH(F)-E11.5-sagittal , FOXB1(D)+SHH(F)-E11.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus, the prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: telencephalon mantle layer Ambiguous FOXB1(D)+SHH(F)-E11.5-sagittal , FOXB1(D)+SHH(F)-E11.5-coronal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer Ambiguous FOXB1(D)+SHH(F)-E11.5-sagittal , FOXB1(D)+SHH(F)-E11.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex and the ganglionic eminences.
Specimen Foxb1 - E12.5: embryonic day 12.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS20: cerebral cortex intermediate zone Ambiguous FOXB1(D)+SHH(F)-E12.5-coronal , FOXB1(D)+SHH(F)-E12.5-sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer Ambiguous FOXB1(D)+SHH(F)-E12.5-coronal , FOXB1(D)+SHH(F)-E12.5-sagittal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS20: cortical plate Ambiguous FOXB1(D)+SHH(F)-E12.5-sagittal , FOXB1(D)+SHH(F)-E12.5-coronal Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer Very strong Non-Uniform FOXB1(D)+SHH(F)-E12.5-sagittal , FOXB1(D)+SHH(F)-E12.5-coronal There is very strong staining in the mantle zone of the mam zone. Staining is either absent or ubiquitous in the mantle zone of the premam and supramam zones and the TT.
TS20: diencephalon ventricular layer Ambiguous FOXB1(D)+SHH(F)-E12.5-sagittal , FOXB1(D)+SHH(F)-E12.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS20: ganglionic eminence Ambiguous FOXB1(D)+SHH(F)-E12.5-sagittal , FOXB1(D)+SHH(F)-E12.5-coronal Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS20: hypothalamus mantle layer Ambiguous FOXB1(D)+SHH(F)-E12.5-coronal , FOXB1(D)+SHH(F)-E12.5-sagittal Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer Ambiguous FOXB1(D)+SHH(F)-E12.5-sagittal , FOXB1(D)+SHH(F)-E12.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: thalamus mantle layer Ambiguous FOXB1(D)+SHH(F)-E12.5-sagittal , FOXB1(D)+SHH(F)-E12.5-coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer Ambiguous FOXB1(D)+SHH(F)-E12.5-sagittal , FOXB1(D)+SHH(F)-E12.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.
Specimen Foxb1 - E14.5: embryonic day 14.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Ambiguous FOXB1(D)+SHH(F)-E14.5-coronal , FOXB1(D)+SHH(F)-E14.5-sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer Ambiguous FOXB1(D)+SHH(F)-E14.5-coronal , FOXB1(D)+SHH(F)-E14.5-sagittal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS22: cortical plate Ambiguous FOXB1(D)+SHH(F)-E14.5-coronal , FOXB1(D)+SHH(F)-E14.5-sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer Very strong Non-Uniform FOXB1(D)+SHH(F)-E14.5-sagittal , FOXB1(D)+SHH(F)-E14.5-coronal There is very strong staining in the mantle zone of the mam zone. Staining is either absent or ubiquitous in the mantle zone of the premam and supramam zones and the TT.
TS22: diencephalon ventricular layer Ambiguous FOXB1(D)+SHH(F)-E14.5-coronal , FOXB1(D)+SHH(F)-E14.5-sagittal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS22: ganglionic eminence Ambiguous FOXB1(D)+SHH(F)-E14.5-coronal , FOXB1(D)+SHH(F)-E14.5-sagittal Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS22: hypothalamus mantle layer Ambiguous FOXB1(D)+SHH(F)-E14.5-sagittal , FOXB1(D)+SHH(F)-E14.5-coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: hypothalamus ventricular layer Ambiguous FOXB1(D)+SHH(F)-E14.5-sagittal , FOXB1(D)+SHH(F)-E14.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: thalamus mantle layer Ambiguous FOXB1(D)+SHH(F)-E14.5-sagittal , FOXB1(D)+SHH(F)-E14.5-coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS22: thalamus ventricular layer Ambiguous FOXB1(D)+SHH(F)-E14.5-sagittal , FOXB1(D)+SHH(F)-E14.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.
Specimen Foxb1 - E16.5: embryonic day 16.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS24: cerebral cortex intermediate zone Ambiguous FOXB1(D)+SHH(F)-E16.5-coronal , FOXB1(D)+SHH(F)-E16.5-sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer Ambiguous FOXB1(D)+SHH(F)-E16.5-sagittal , FOXB1(D)+SHH(F)-E16.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS24: cortical plate Ambiguous FOXB1(D)+SHH(F)-E16.5-sagittal , FOXB1(D)+SHH(F)-E16.5-coronal Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer Very strong Non-Uniform FOXB1(D)+SHH(F)-E16.5-sagittal , FOXB1(D)+SHH(F)-E16.5-coronal There is very strong staining in the mantle zone of the mam zone. Staining is either absent or ubiquitous in the mantle zone of the premam and supramam zones.
TS24: diencephalon ventricular layer Ambiguous FOXB1(D)+SHH(F)-E16.5-coronal , FOXB1(D)+SHH(F)-E16.5-sagittal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones.
TS24: ganglionic eminence Ambiguous FOXB1(D)+SHH(F)-E16.5-sagittal , FOXB1(D)+SHH(F)-E16.5-coronal Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS24: hypothalamus mantle layer Ambiguous FOXB1(D)+SHH(F)-E16.5-sagittal , FOXB1(D)+SHH(F)-E16.5-coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: hypothalamus ventricular layer Ambiguous FOXB1(D)+SHH(F)-E16.5-coronal , FOXB1(D)+SHH(F)-E16.5-sagittal Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: thalamus mantle layer Ambiguous FOXB1(D)+SHH(F)-E16.5-sagittal , FOXB1(D)+SHH(F)-E16.5-coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS24: thalamus ventricular layer Ambiguous FOXB1(D)+SHH(F)-E16.5-coronal , FOXB1(D)+SHH(F)-E16.5-sagittal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.
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