Gene Expression Data
Assay Details
Assay
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Reference:
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J:151956
Crist CG, et al., Muscle stem cell behavior is modified by microRNA-27 regulation of Pax3 expression. Proc Natl Acad Sci U S A. 2009 Aug 11;106(32):13383-7
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4358070
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Gene symbol:
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Mir27b
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Gene name:
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microRNA 27b
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Probe:
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miR-27b probe
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Probe preparation:
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Antisense labelled with digoxigenin
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A:
embryonic day 10.5
(more )
Specimen
2A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2B:
embryonic day 10.5
(more )
Specimen
2B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS17: sclerotome |
Present |
Regionally restricted |
2B
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In immature, caudal somites, expression is present in the ventral sclerotome.
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TS17: dermomyotome |
Absent |
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2B
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Expression is excluded from the dorsal dermomyotome.
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Specimen
2C:
embryonic day 10.5
(more )
Specimen
2C:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS17: myotome |
Present |
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2C
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In more mature somites, expression is present in the myotome, where skeletal muscle is forming. It is present in epaxial and hypaxial lips, as well as interspersed cells in the myotome.
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TS17: dermatome |
Absent |
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2C
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