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Gene Expression Data
Assay Details
Assay
Reference: J:143535 Toyoda S, et al., Sohlh2 affects differentiation of KIT positive oocytes and spermatogonia. Dev Biol. 2009 Jan 1;325(1):238-48
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3839571
Gene symbol: Kit
Gene name: KIT proto-oncogene receptor tyrosine kinase
Antibody: anti-CD117 (ACK2)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1E, 1F: postnatal week 10 (more )
Note: Double labeled: green - Kit; red - Sohlh2 (assay MGI:3839434).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted 1F , 1E Expression was detected in type A differentiated spermatogonia and a type B spermatogonium or preleptotene spermatocyte.
Specimen 1I, 1J: postnatal week 7 (more )
Note: Double labeled: green - Kit; red - Sohlh2 (assay MGI:3839434).
Structure Level Pattern Image Note
TS28: oocyte Present 1J , 1I
Specimen 1L, 1M: postnatal day 0 (more )
Note: Double labeled: green - Kit; red - Sohlh2 (assay MGI:3839434).
Structure Level Pattern Image Note
TS27: ovary Present 1L , 1M
Specimen 3A: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: oocyte Present 3A
Specimen 3B: postnatal day 0; Sohlh2tm1Miya/Sohlh2tm1Miya (more )
Structure Level Pattern Image Note
TS27: oocyte Present 3B The number of oocytes stained was reduced to approximately one-tenth of that in the +/+ ovary.
Specimen 5G: postnatal week 7 (more )
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted 5G Expression was detected in type A differentiated spermatogonia.
Specimen 5H: postnatal week 7; Sohlh2tm1Miya/Sohlh2tm1Miya (more )
Structure Level Pattern Image Note
TS28: spermatogonium Trace 5H Almost no positive cells were found in the tubules.
Specimen 5I: postnatal week 7 (more )
Note: Double labeled: green - Kit; red - Cdh1 (assay MGI:3839438).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted 5I Expression was detected in type A differentiated spermatogonia.
Specimen 5J: postnatal week 7; Sohlh2tm1Miya/Sohlh2tm1Miya (more )
Note: Double labeled: green - Kit; red - Cdh1 (assay MGI:3839438).
Structure Level Pattern Image Note
TS28: spermatogonium Trace 5J Almost no positive cells were found in the tubules.
Specimen S6B,6C: postnatal week 10 (more )
Note: Double labeled: green - Kit; red - Sohlh2 (assay MGI:3839434).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S6C , S6B Expression was detected in type A differentiated spermatogonia through all stages of the seminiferous epithelial cycle.
Specimen S6F,6G: postnatal week 10 (more )
Note: Double labeled: green - Kit; red - Sohlh2 (assay MGI:3839434).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S6G , S6F Expression was detected in type A differentiated spermatogonia through all stages of the seminiferous epithelial cycle.
Specimen S6J,6K: postnatal week 10 (more )
Note: Double labeled: green - Kit; red - Sohlh2 (assay MGI:3839434).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S6J , S6K Expression was detected in type A differentiated spermatogonia through all stages of the seminiferous epithelial cycle.
Specimen S8A: postnatal day 12 (more )
Structure Level Pattern Image Note
TS28: testis Present S8A
Specimen S8B: postnatal day 12; Sohlh2tm1Miya/Sohlh2tm1Miya (more )
Structure Level Pattern Image Note
TS28: testis Present S8B A significant number of positive cells are detected in the prepubertal testes.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory