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Gene Expression Data
Assay Details
Assay
Reference: J:143535 Toyoda S, et al., Sohlh2 affects differentiation of KIT positive oocytes and spermatogonia. Dev Biol. 2009 Jan 1;325(1):238-48
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3839434
Gene symbol: Sohlh2
Gene name: spermatogenesis and oogenesis specific basic helix-loop-helix 2
Antibody: Anti-Sohlh2
Detection system: Secondary antibody coupled to Alexa Fluor 568
Results
Specimen 1A, 1C: postnatal week 10 (more )
Note: Double labeled: red - Sohlh2; green - Cdh1 (assay MGI:3839438).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted 1C , 1A Expression was detected in type A undifferentiated spermatogonia. Co-localization was seen with Cdh1.
Specimen 1D, 1F: postnatal week 10 (more )
Note: Double labeled: red - Sohlh2; green - Kit (assay MGI:3839571).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted 1D , 1F Expression was detected in type A spermatogonia but not in type B spermatogonia or preleptotene spermatocytes.
Specimen 1H, 1J: postnatal week 7 (more )
Note: Double labeled: red - Sohlh2; green - Kit (assay MGI:3839571).
Structure Level Pattern Image Note
TS28: oocyte Present 1J , 1H Expression was detected in oocytes in the primordial to primary follicle stages.
TS28: ovary Present 1J , 1H Some co-localization was seen with Kit.
Specimen 1K, 1M: postnatal day 0 (more )
Note: Double labeled: red - Sohlh2; green - Kit (assay MGI:3839571).
Structure Level Pattern Image Note
TS27: ovary Present 1K , 1M Some co-localization was seen with Kit.
Specimen S2B: postnatal day 10 (more )
Structure Level Pattern Image Note
TS28: testis Present S2B
Specimen S2D: postnatal day 10; Sohlh2tm1Miya/Sohlh2tm1Miya (more )
Structure Level Pattern Image Note
TS28: testis Absent S2D
Specimen S2F: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: ovary Present S2F
Specimen S2H: postnatal day 0; Sohlh2tm1Miya/Sohlh2tm1Miya (more )
Structure Level Pattern Image Note
TS27: ovary Absent S2H
Specimen S4A,C: postnatal week 10 (more )
Note: Double labeled: red - Sohlh2; green - Cdh1 (assay MGI:3839438).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S4C , S4A Expression was detected in type A undifferentiated spermatogonia. Co-localization was seen with Cdh1.
Specimen S4E,G: postnatal week 10 (more )
Note: Double labeled: red - Sohlh2; green - Cdh1 (assay MGI:3839438).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S4G , S4E Expression was detected in type A undifferentiated spermatogonia. Co-localization was seen with Cdh1.
Specimen S4I,K: postnatal week 10 (more )
Note: Double labeled: red - Sohlh2; green - Cdh1 (assay MGI:3839438).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S4K , S4I Expression was detected in type A undifferentiated spermatogonia. Co-localization was seen with Cdh1, but some Cdh1 positive cells did not express Sohlh2.
Specimen S5A,C: postnatal week 10 (more )
Note: Double labeled: red - Sohlh2; green - Cdh1 (assay MGI:3839438).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S5C , S5A Expression was detected in type A single spermatogonia. Co-localization was seen with Cdh1 but not all Cdh1+ spermatogonia expressed Sohlh2.
Specimen S5D,F: postnatal week 10 (more )
Note: Double labeled: red - Sohlh2; green - Cdh1 (assay MGI:3839438).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S5F , S5D Expression was detected in type A paired spermatogonia. Co-localization was seen with Cdh1 but not all Cdh1+ spermatogonia expressed Sohlh2.
Specimen S5G,I: postnatal week 10 (more )
Note: Double labeled: red - Sohlh2; green - Cdh1 (assay MGI:3839438).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S5G , S5I Expression was detected in type A aligned spermatogonia. Co-localization was seen with Cdh1 but not all Cdh1+ spermatogonia expressed Sohlh2.
Specimen S5J,L: postnatal week 10 (more )
Note: Double labeled: red - Sohlh2; green - Cdh1 (assay MGI:3839438).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S5L , S5J Expression was detected in type A aligned spermatogonia at the 16-cell stage. Co-localization was seen with Cdh1 but not all Cdh1+ spermatogonia expressed Sohlh2.
Specimen S6A,C: postnatal week 10 (more )
Note: Double labeled: red - Sohlh2; green - Kit (assay MGI:3839571).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S6C , S6A Expression was detected in type A differentiated spermatogonia through all stages of the seminiferous epithelial cycle. Co-localization was seen with Kit.
Specimen S6E,G: postnatal week 10 (more )
Note: Double labeled: red - Sohlh2; green - Kit (assay MGI:3839571).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S6G , S6E Expression was detected in type A differentiated spermatogonia through all stages of the seminiferous epithelial cycle. Co-localization was seen with Kit.
Specimen S6I,K: postnatal week 10 (more )
Note: Double labeled: red - Sohlh2; green - Kit (assay MGI:3839571).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S6I , S6K Expression was detected in type A differentiated spermatogonia through all stages of the seminiferous epithelial cycle. Co-localization was seen with Kit.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
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