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Targeted Allele Detail
Symbol: Sohlh2tm1Miya
Name: spermatogenesis and oogenesis specific basic helix-loop-helix 2; targeted mutation 1, Jun-ichi Miyazaki
MGI ID: MGI:3829597
Gene: Sohlh2  Location: Chr3:55089465-55117378 bp, + strand  Genetic Position: Chr3, 26.53 cM
Alliance: Sohlh2tm1Miya page
Germline Transmission:  Earliest citation of germline transmission: J:143535
Parent Cell Line:  EB3 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsExons 2 through 8 were replaced with an IRES beta-geo cassette and puromycin selection marker. The absence of protein expression was confirmed by western blot analysis on testes extracts. (J:143535)
View phenotypes and curated references for all genotypes (concatenated display).
In Mice Carrying this Mutation: 49 assay results
In Structures Affected by this Mutation: 4 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Sohlh2 Mutation:  27 strains or lines available
ES cell line = EB3, derived from E14TG2a. EB3 cells carry one allele of Pou5f1 that has been inactivated via targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. J:143535 does not mention whether the null allele of Pou5f1 is also present in the line of mice created.
Original:  J:143535 Toyoda S, et al., Sohlh2 affects differentiation of KIT positive oocytes and spermatogonia. Dev Biol. 2009 Jan 1;325(1):238-48
All:  1 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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MGI 6.13
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