About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:143535 Toyoda S, et al., Sohlh2 affects differentiation of KIT positive oocytes and spermatogonia. Dev Biol. 2009 Jan 1;325(1):238-48
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3839434
Gene symbol: Sohlh2
Gene name: spermatogenesis and oogenesis specific basic helix-loop-helix 2
Antibody: Anti-Sohlh2
Detection system: Secondary antibody coupled to Alexa Fluor 568
Results
Specimen 1A, 1C: postnatal week 10 (more )
Note: Double labeled: red - Sohlh2; green - Cdh1 (assay MGI:3839438).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted 1C , 1A Expression was detected in type A undifferentiated spermatogonia. Co-localization was seen with Cdh1.
Specimen 1D, 1F: postnatal week 10 (more )
Note: Double labeled: red - Sohlh2; green - Kit (assay MGI:3839571).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted 1D , 1F Expression was detected in type A spermatogonia but not in type B spermatogonia or preleptotene spermatocytes.
Specimen 1H, 1J: postnatal week 7 (more )
Note: Double labeled: red - Sohlh2; green - Kit (assay MGI:3839571).
Structure Level Pattern Image Note
TS28: oocyte Present 1J , 1H Expression was detected in oocytes in the primordial to primary follicle stages.
TS28: ovary Present 1J , 1H Some co-localization was seen with Kit.
Specimen 1K, 1M: postnatal day 0 (more )
Note: Double labeled: red - Sohlh2; green - Kit (assay MGI:3839571).
Structure Level Pattern Image Note
TS27: ovary Present 1K , 1M Some co-localization was seen with Kit.
Specimen S2B: postnatal day 10 (more )
Structure Level Pattern Image Note
TS28: testis Present S2B
Specimen S2D: postnatal day 10; Sohlh2tm1Miya/Sohlh2tm1Miya (more )
Structure Level Pattern Image Note
TS28: testis Absent S2D
Specimen S2F: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: ovary Present S2F
Specimen S2H: postnatal day 0; Sohlh2tm1Miya/Sohlh2tm1Miya (more )
Structure Level Pattern Image Note
TS27: ovary Absent S2H
Specimen S4A,C: postnatal week 10 (more )
Note: Double labeled: red - Sohlh2; green - Cdh1 (assay MGI:3839438).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S4C , S4A Expression was detected in type A undifferentiated spermatogonia. Co-localization was seen with Cdh1.
Specimen S4E,G: postnatal week 10 (more )
Note: Double labeled: red - Sohlh2; green - Cdh1 (assay MGI:3839438).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S4G , S4E Expression was detected in type A undifferentiated spermatogonia. Co-localization was seen with Cdh1.
Specimen S4I,K: postnatal week 10 (more )
Note: Double labeled: red - Sohlh2; green - Cdh1 (assay MGI:3839438).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S4K , S4I Expression was detected in type A undifferentiated spermatogonia. Co-localization was seen with Cdh1, but some Cdh1 positive cells did not express Sohlh2.
Specimen S5A,C: postnatal week 10 (more )
Note: Double labeled: red - Sohlh2; green - Cdh1 (assay MGI:3839438).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S5C , S5A Expression was detected in type A single spermatogonia. Co-localization was seen with Cdh1 but not all Cdh1+ spermatogonia expressed Sohlh2.
Specimen S5D,F: postnatal week 10 (more )
Note: Double labeled: red - Sohlh2; green - Cdh1 (assay MGI:3839438).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S5F , S5D Expression was detected in type A paired spermatogonia. Co-localization was seen with Cdh1 but not all Cdh1+ spermatogonia expressed Sohlh2.
Specimen S5G,I: postnatal week 10 (more )
Note: Double labeled: red - Sohlh2; green - Cdh1 (assay MGI:3839438).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S5G , S5I Expression was detected in type A aligned spermatogonia. Co-localization was seen with Cdh1 but not all Cdh1+ spermatogonia expressed Sohlh2.
Specimen S5J,L: postnatal week 10 (more )
Note: Double labeled: red - Sohlh2; green - Cdh1 (assay MGI:3839438).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S5L , S5J Expression was detected in type A aligned spermatogonia at the 16-cell stage. Co-localization was seen with Cdh1 but not all Cdh1+ spermatogonia expressed Sohlh2.
Specimen S6A,C: postnatal week 10 (more )
Note: Double labeled: red - Sohlh2; green - Kit (assay MGI:3839571).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S6C , S6A Expression was detected in type A differentiated spermatogonia through all stages of the seminiferous epithelial cycle. Co-localization was seen with Kit.
Specimen S6E,G: postnatal week 10 (more )
Note: Double labeled: red - Sohlh2; green - Kit (assay MGI:3839571).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S6G , S6E Expression was detected in type A differentiated spermatogonia through all stages of the seminiferous epithelial cycle. Co-localization was seen with Kit.
Specimen S6I,K: postnatal week 10 (more )
Note: Double labeled: red - Sohlh2; green - Kit (assay MGI:3839571).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S6I , S6K Expression was detected in type A differentiated spermatogonia through all stages of the seminiferous epithelial cycle. Co-localization was seen with Kit.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory