Probe:	Sepp cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS11: ectoplacental cone	Strong		2A
TS11: trophectoderm	Strong		2A
TS28: decidua	Strong		2A
TS11: neural ectoderm	Weak		2A

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present		2B
TS13: future brain neural fold	Present		2B

Structure	Level	Pattern	Image	Note
TS13: ectoplacental cone	Present		2C
TS13: neural tube	Present		2C

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Strong		2D
TS15: future forebrain	Present		2D	Expression is present in the prosencephalon.
TS15: future midbrain	Present		2D	Expression is present in the mesencephalon.
TS15: future hindbrain	Present		2D	Expression is present in the metencephalon.
TS15: future spinal cord	Present		2D	Expression is present in the dorsal neural tube.
TS15: heart	Present		2D

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Strong		2E
TS17: telencephalon	Present		2E
TS17: midbrain	Present		2E	Expression is present in the mesencephalon.
TS17: hindbrain	Present		2E	Expression is present in the metencephalon.
TS17: future spinal cord	Present		2E	Expression is present in the dorsal neural tube.
TS17: forelimb bud	Present		2E
TS17: hindlimb bud	Present		2E

Structure	Level	Pattern	Image	Note
TS19: central nervous system	Strong		2F
TS19: telencephalon	Present		2F
TS19: midbrain	Present		2F	Expression is present in the mesencephalon.
TS19: metencephalon	Present		2F	Expression is present in the metencephalon.
TS19: future spinal cord	Present		2F	Expression is present in the dorsal neural tube.
TS19: forelimb bud	Strong		2F
TS19: hindlimb bud	Strong		2F

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Strong		2G
TS20: telencephalon	Present		2G
TS20: midbrain	Present		2G	Expression is present in the mesencephalon.
TS20: metencephalon	Present		2G	Expression is present in the metencephalon.
TS20: spinal cord	Present		2G	Expression is present in the dorsal neural tube.
TS20: forelimb	Strong		2G
TS20: hindlimb	Strong		2G

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Strong
TS21: lung epithelium	Strong
TS21: lung mesenchyme	Moderate
TS21: liver	Strong
TS21: pancreas	Moderate	Regionally restricted		There is moderate expression in the exocrine portion.
TS21: renal corpuscle	Moderate
TS21: renal tubule	Moderate
TS21: intestine epithelium	Moderate			There is moderate expression in the surface epithelium.
TS21: intestine mesenchyme	Moderate			There is moderate expression in the muscular layer.
TS21: skin	Weak
TS21: integumental system	Weak	Regionally restricted		There is weak expression in the hair follicle.
TS21: heart	Weak
TS21: blood	Very strong

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Strong
TS22: lung epithelium	Strong
TS22: lung mesenchyme	Moderate
TS22: liver	Strong
TS22: pancreas	Moderate	Regionally restricted		There is moderate expression in the exocrine portion.
TS22: renal corpuscle	Moderate
TS22: renal tubule	Moderate
TS22: intestine epithelium	Moderate			There is moderate expression in the surface epithelium.
TS22: intestine mesenchyme	Moderate			There is moderate expression in the muscular layer.
TS22: hair follicle	Moderate
TS22: skin	Moderate
TS22: heart	Weak
TS22: blood	Very strong

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Strong
TS23: lung epithelium	Strong
TS23: lung mesenchyme	Moderate
TS23: liver	Strong
TS23: pancreas	Moderate	Regionally restricted		There is moderate expression in the exocrine portion.
TS23: renal corpuscle	Moderate
TS23: renal tubule	Moderate
TS23: intestine epithelium	Moderate			There is moderate expression in the surface epithelium.
TS23: intestine mesenchyme	Moderate			There is moderate expression in the muscular layer.
TS23: hair follicle	Moderate
TS23: skin	Moderate
TS23: heart	Weak
TS23: blood	Very strong

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Strong
TS24: lung epithelium	Strong
TS24: lung mesenchyme	Moderate
TS24: liver	Strong
TS24: pancreas	Strong	Regionally restricted		There is strong expression in the exocrine portion.
TS24: renal corpuscle	Strong
TS24: renal tubule	Moderate
TS24: intestine epithelium	Strong			There is strong expression in the surface epithelium.
TS24: intestine mesenchyme	Strong			There is strong expression in the muscular layer.
TS24: detrusor muscle of bladder	Strong
TS24: urothelium of bladder	Strong
TS24: hair follicle	Strong
TS24: skin	Strong
TS24: heart	Weak
TS24: blood	Very strong

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Strong
TS25: lung epithelium	Moderate
TS25: lung mesenchyme	Moderate
TS25: liver	Moderate
TS25: pancreas	Strong	Regionally restricted		There is strong expression in the exocrine portion.
TS25: renal corpuscle	Strong
TS25: renal tubule	Moderate
TS25: intestine epithelium	Strong			There is strong expression in the surface epithelium.
TS25: intestine mesenchyme	Strong			There is strong expression in the muscular layer.
TS25: urothelium of bladder	Strong
TS25: detrusor muscle of bladder	Moderate
TS25: hair follicle	Strong
TS25: skin	Strong
TS25: heart	Weak
TS25: blood	Very strong

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Strong
TS26: lung epithelium	Moderate
TS26: lung mesenchyme	Moderate
TS26: liver	Moderate
TS26: pancreas	Strong	Regionally restricted		There is strong expression in the exocrine portion.
TS26: renal corpuscle	Strong
TS26: renal tubule	Moderate
TS26: intestine epithelium	Strong			There is strong expression in the surface epithelium.
TS26: intestine mesenchyme	Strong			There is strong expression in the muscular layer.
TS26: urothelium of bladder	Strong
TS26: detrusor muscle of bladder	Moderate
TS26: hair follicle	Strong
TS26: skin	Strong
TS26: heart	Weak
TS26: blood	Very strong

Structure	Level	Pattern	Image	Note
TS22: metencephalon	Present	Diffuse	3A-1

Structure	Level	Pattern	Image	Note
TS23: telencephalon	Present	Diffuse	3A-2
TS23: cerebral cortex marginal layer	Present		3A-2

Structure	Level	Pattern	Image	Note
TS24: metencephalon	Present	Diffuse	3A-3
TS24: cerebral cortex marginal layer	Present		3A-3

Structure	Level	Pattern	Image	Note
TS21: heart	Weak		3B-1
TS21: blood	Strong		3B-1

Structure	Level	Pattern	Image	Note
TS23: heart	Weak		3B-2
TS23: blood	Strong		3B-2

Structure	Level	Pattern	Image	Note
TS24: heart	Weak		3B-3
TS24: blood	Strong		3B-3

Structure	Level	Pattern	Image	Note
TS21: liver	Strong	Diffuse	3C-1

Structure	Level	Pattern	Image	Note
TS23: liver	Strong	Diffuse	3C-2

Structure	Level	Pattern	Image	Note
TS25: liver	Present		3C-3

Structure	Level	Pattern	Image	Note
TS21: intestine	Present	Diffuse	3D-1

Structure	Level	Pattern	Image	Note
TS23: intestine	Present	Diffuse	3D-2

Structure	Level	Pattern	Image	Note
TS26: intestine	Present	Diffuse	3D-3

Structure	Level	Pattern	Image	Note
TS21: lung epithelium	Present		3E-1	Expression is present at a higher level in the bronchial epithelium than in the mesenchyme.
TS21: lung mesenchyme	Present		3E-1	Expression is present at a higher level in the bronchial epithelium than in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS23: lung epithelium	Present		3E-2	Expression is present at a higher level in the bronchial epithelium than in the mesenchyme.
TS23: lung mesenchyme	Present		3E-2	Expression is present at a higher level in the bronchial epithelium than in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS25: lung epithelium	Present	Diffuse	3E-3
TS25: lung mesenchyme	Present	Diffuse	3E-3

Structure	Level	Pattern	Image	Note
TS21: renal corpuscle	Strong		3F-1
TS21: renal tubule	Strong		3F-1
TS21: mesenchyme of metanephros	Weak		3F-1

Structure	Level	Pattern	Image	Note
TS23: renal corpuscle	Strong		3F-2
TS23: renal tubule	Strong		3F-2
TS23: mesenchyme of metanephros	Weak		3F-2

Structure	Level	Pattern	Image	Note
TS24: renal corpuscle	Strong		3F-3
TS24: renal tubule	Strong		3F-3
TS24: mesenchyme of metanephros	Weak		3F-3

Structure	Level	Pattern	Image	Note
TS21: pancreas	Present	Non-Uniform	3G-1	Expression levels are higher in the acinar cells.
TS21: intestine	Present		3G-1
TS21: liver	Present		3G-1

Structure	Level	Pattern	Image	Note
TS23: pancreas	Present	Non-Uniform	3G-2	Expression levels are higher in the acinar cells.

Structure	Level	Pattern	Image	Note
TS25: pancreas	Present	Non-Uniform	3G-3	Expression levels are higher in the acinar cells.

Structure	Level	Pattern	Image	Note
TS21: submandibular gland primordium	Present		3H-1

Structure	Level	Pattern	Image	Note
TS24: submandibular gland	Present		3H-2

Structure	Level	Pattern	Image	Note
TS25: submandibular gland	Present		3H-3

Structure	Level	Pattern	Image	Note
TS21: skin	Present		3I-1

Structure	Level	Pattern	Image	Note
TS25: skin	Present		3I-2

Structure	Level	Pattern	Image	Note
TS26: skin	Present		3I-3

Structure	Level	Pattern	Image	Note
TS22: vibrissa	Present		3J-1	Expression is present in the whisker follicle.

Structure	Level	Pattern	Image	Note
TS24: vibrissa	Present		3J-2	Expression is present in the whisker follicle.

Structure	Level	Pattern	Image	Note
TS25: vibrissa	Present		3J-3	Expression is present in the whisker follicle.

Structure	Level	Pattern	Image	Note
TS24: bladder	Present		3K-1

Structure	Level	Pattern	Image	Note
TS25: bladder	Present	Non-Uniform	3K-2	Expression is higher in the transitional epithelium than the muscle layer.

Structure	Level	Pattern	Image	Note
TS26: bladder	Present	Non-Uniform	3K-3	Expression is higher in the transitional epithelium than the muscle layer.

Structure	Level	Pattern	Image	Note
TS22: hindlimb digit	Present		3L

Structure	Cell Type	Level	Pattern	Image	Note
TS23: Leydig cell	Leydig cell	Present		3M

Structure	Level	Pattern	Image	Note
TS24: inner ear epithelium	Present		3N	Expression is present in the sensory epithelium.