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Gene Expression Data
Assay Details
Assay
Reference: J:83255 Chi CL, et al., The isthmic organizer signal FGF8 is required for cell survival in the prospective midbrain and cerebellum. Development. 2003 Jun;130(12):2633-44
Assay type: Immunohistochemistry
MGI Accession ID: MGI:2677310
Gene symbol: Th
Gene name: tyrosine hydroxylase
Antibody: anti-tyrosine hydroxylase
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes: Assays used Clarke's fixative (75% ethanol, 25% glacial acetic acid)
Results
Specimen 2C': embryonic day 17.5 (more )
Note: Authors state that heterozygous and wild-type littermates were used as control embryos.
Structure Level Pattern Image Note
TS25: midbrain tegmentum Present 2C'
TS25: hindbrain Present Regionally restricted 2C' Expression in present in the anterior.
Specimen 2D': embryonic day 17.5; Fgf8tm1.1Mrt/Fgf8tm1.1Mrt (more )
Structure Level Pattern Image Note
TS25: hindbrain Present Regionally restricted 2D' In the mutant, the areas of expression are reduced in size.
TS25: midbrain tegmentum Present Regionally restricted 2D' In the mutant, the areas of expression are reduced in size.
Specimen 2E': embryonic day 17.5; Fgf8tm1.1Mrt/Fgf8tm1.4Mrt (more )
Structure Level Pattern Image Note
TS25: hindbrain Absent 2E' There are severe phenotypic defects in these animals.
TS25: midbrain tegmentum Absent 2E' There are severe phenotypic defects in these animals.
Specimen 2F': embryonic day 17.5; Fgf8tm1.3Mrt/Fgf8tm1.4Mrt, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS25: hindbrain Absent 2F' There are severe phenotypic defects in these animals.
TS25: midbrain tegmentum Absent 2F' There are severe phenotypic defects in these animals.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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