Fgf8^tm1.1Mrt
Targeted Allele Detail

Summary

• Symbol: Fgf8^tm1.1Mrt
• Name: fibroblast growth factor 8; targeted mutation 1.1, Gail R Martin
• MGI ID: MGI:1857843
• Synonyms: Fgf8neo, Fgf8^neo
• Gene: Fgf8 Location: Chr19:45725237-45731354 bp, - strand Genetic Position: Chr19, 38.75 cM, cytoband C3-D
• Alliance: Fgf8^tm1.1Mrt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:45909
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph)
• Mutation: Insertion
• Mutation details: Insertion of an frt-flanked neomycin cassette into intron 1 and loxP sites flanking exons 2 and 3. In homozygous embryos, both wild-type and aberrantly spliced mRNAs are produced. Less than 45% of the normal levels of wild-type mRNA is produced from this allele. This allele behaves as a hypomorphic allele. (J:45909)

Expression

In Mice Carrying this Mutation:	21 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fgf8 Mutation: 18 strains or lines available

Notes

One of three alleles produced using a targeting construct capable of inducing different allelic variants when mated to mice that express cre, flp, or both. Referred to in the literature as Fgf8^neo, the presence of the neo cassette in the intron cause a reduction in the amount of Fgf8 expression and is thus considered a hypomorphic allele. Heterozygotes are normal. Homozygotes die within one day of birth. Gastrulation and limb development appear normal, but aspects of brain development are perturb.

References

• Original: J:45909 Meyers EN, et al., An Fgf8 mutant allelic series generated by Cre- and Flp-mediated recombination. Nat Genet. 1998 Feb;18(2):136-41
• All: 41 reference(s)