Gene Expression Data
Assay Details
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Assay
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Reference:
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J:83255
Chi CL, et al., The isthmic organizer signal FGF8 is required for cell survival in the prospective midbrain and cerebellum. Development. 2003 Jun;130(12):2633-44
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2677308
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Gene symbol:
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Otx2
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Gene name:
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orthodenticle homeobox 2
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Probe:
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Otx2 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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A mixture of two in situ probes was used in this assay.
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Results
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Specimen
5O:
embryonic day 8.0
(more  )
Note: 7 somite stage embryo. Littermate control; exact genotype not specified. Also stained for Hoxa2 (assay MGI:2677309)
Specimen
5O:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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7 somite stage embryo. Littermate control; exact genotype not specified. Also stained for Hoxa2 (assay MGI:2677309)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future midbrain |
Present |
Regionally restricted |
5O
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Expression is present in a domain that stretches from the rostral end of prospective forebrain to caudal end of prospective midbrain.
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| TS12: future prosencephalon |
Present |
Regionally restricted |
5O
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Expression is present in a domain that stretches from the rostral end of prospective forebrain to caudal end of prospective midbrain.
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Specimen
5P:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Fgf8tm1.3Mrt/Fgf8tm1.4Mrt
En1tm2(cre)Wrst/En1+
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Conditional mutant. 7 somite stage embryo. Also stained for Hoxa2 (assay MGI:2677309)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future midbrain |
Present |
Regionally restricted |
5P
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As in controls, expression is present in a domain that stretches from the rostral end of prospective forebrain to caudal end of prospective midbrain.
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| TS12: future prosencephalon |
Present |
Regionally restricted |
5P
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As in controls, expression is present in a domain that stretches from the rostral end of prospective forebrain to caudal end of prospective midbrain.
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Specimen
5W:
embryonic day 8.5
(more )
Note: 12 somite stage embryo. Littermate control; exact genotype not specified. Also stained for Hoxa2 (assay MGI:2677309)
Specimen
5W:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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12 somite stage embryo. Littermate control; exact genotype not specified. Also stained for Hoxa2 (assay MGI:2677309)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future midbrain |
Present |
Regionally restricted |
5W
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Expression is present in a domain that stretches from the rostral end of prospective forebrain to caudal end of prospective midbrain.
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| TS13: future prosencephalon |
Present |
Regionally restricted |
5W
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Expression is present in a domain that stretches from the rostral end of prospective forebrain to caudal end of prospective midbrain.
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Specimen
5X:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Fgf8tm1.3Mrt/Fgf8tm1.4Mrt
En1tm2(cre)Wrst/En1+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Conditional mutant. 13 somite stage embryo. Also stained for Hoxa2 (assay MGI:2677309)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future midbrain |
Present |
Regionally restricted |
5X
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Expression is present in a domain that stretches from the rostral end of prospective forebrain to caudal end of prospective midbrain. However, the caudal end of the prospective midbrain appears to be reduced in size and/or expression compared to controls.
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| TS13: future prosencephalon |
Present |
Regionally restricted |
5X
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Expression is present in a domain that stretches from the rostral end of prospective forebrain to caudal end of prospective midbrain. However, the caudal end of the prospective midbrain appears to be reduced in size and/or expression compared to controls.
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