Probe:	AP-2.2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS10: ectoplacental cavity	Present		1A
TS10: primary trophoblast giant cell	Present		1A
TS10: extraembryonic component	Present	Regionally restricted	1A	Expression is in the chorion.
TS28: decidua	Present		1A

Structure	Level	Pattern	Image	Note
TS13: extraembryonic component	Strong	Regionally restricted	1B	Expression is in trophoblast giant cells.
TS13: future spinal cord neural fold	Present	Regionally restricted	1B	Expression is at the dorsal edge at both cranial and trunk levels.
TS13: neural ectoderm	Present	Regionally restricted	1B	Expression is at the dorsal edge at both cranial and trunk levels.
TS13: neural tube	Present	Regionally restricted	1B	Expression is at the dorsal edge at both cranial and trunk levels.
TS13: head mesenchyme derived from neural crest	Present		1B
TS28: decidua	Strong		1B

Structure	Level	Pattern	Image	Note
TS13: extraembryonic component	Strong	Regionally restricted	1C	Expression is in trophoblast giant cells.
TS28: decidua	Strong		1C

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mandibular component mesenchyme derived from neural crest	Present	Regionally restricted	3A
TS15: 1st branchial arch maxillary component mesenchyme derived from neural crest	Present	Regionally restricted	3A
TS15: 2nd branchial arch mesenchyme derived from neural crest	Present	Regionally restricted	3A
TS15: fronto-nasal process mesenchyme	Present	Regionally restricted	3A
TS15: future forebrain	Present		3A
TS15: hindlimb ridge mesenchyme	Present		3A

Structure	Level	Pattern	Image	Note
TS19: mandibular process	Present	Regionally restricted	3B	Expression is rostral in the mandibular and caudal in the maxillary swellings.
TS19: maxillary process	Present	Regionally restricted	3B	Expression is rostral in the mandibular and caudal in the maxillary swellings.
TS19: telencephalon	Present	Regionally restricted	3B	Expression is in the wall with a boundary that corresponds to the limit between the walls and the striatum.

Structure	Level	Pattern	Image	Note
TS19: fronto-nasal process	Present	Regionally restricted	3C	Expression is in the mesenchyme surrounding the developing nasal cavities and in the presumptive respiratory epithelium.
TS19: telencephalon	Present	Regionally restricted	3C	Expression is in the wall.

Structure	Level	Pattern	Image	Note
TS19: mandibular process	Present	Regionally restricted	3D	Expression is rostral in the mandibular and caudal in the maxillary swellings.
TS19: maxillary process	Present	Regionally restricted	3D	Expression is rostral in the mandibular and caudal in the maxillary swellings.
TS19: naso-lacrimal groove	Present	Regionally restricted	3D	Expression is in the mesenchyme.
TS19: fronto-nasal process	Present	Regionally restricted	3D	Expression is in the mesenchyme surrounding the developing nasal cavities and in the presumptive respiratory epithelium.
TS19: telencephalon	Present	Regionally restricted	3D	Expression is in the wall with a boundary that corresponds to the limit between the walls and the striatum.

Structure	Level	Pattern	Image	Note
TS19: mandibular process	Present	Regionally restricted	3E	Expression is rostral in the mandibular and caudal in the maxillary swellings.
TS19: maxillary process	Present	Regionally restricted	3E	Expression is rostral in the mandibular and caudal in the maxillary swellings.
TS19: dental placode	Present	Regionally restricted	3E	Expression is in the area where the tooth buds will form.
TS19: tongue	Present		3E
TS19: naso-lacrimal groove	Present	Regionally restricted	3E	Expression is in the mesenchyme.
TS19: surface ectoderm	Present	Regionally restricted	3E	Expression is in the face.
TS19: oronasal cavity	Present	Regionally restricted	3E	Expression is in the epithelium of the mouth cavity down to the oesophagus.
TS19: telencephalon	Present	Regionally restricted	3E	Expression is in the wall.

Structure	Level	Pattern	Image	Note
TS19: naso-lacrimal groove	Present	Regionally restricted	3F	Expression is in the mesenchyme.
TS19: surface ectoderm	Present	Regionally restricted	3F	Expression is in the face.
TS19: oronasal cavity	Present	Regionally restricted	3F	Expression is in the epithelium of the mouth cavity down to the oesophagus.
TS19: telencephalon	Present	Regionally restricted	3F	Expression is in the wall.
TS19: diencephalon	Present	Regionally restricted	3F	Expression is within the ventral thalamus.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Strong		4A

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud mesenchyme	Strong	Regionally restricted	4B	Expression is strongest in distal cells underlying the apical ectodermal ridge.
TS17: hindlimb bud ectoderm	Absent		4B
TS17: forelimb bud mesenchyme	Present	Regionally restricted	4B	Expression is restricted to the distal "progress zone".

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	4C	Expression is in two thin symmetrical cell layers near the dorsal margin of the spinal cord.
TS19: handplate mesenchyme	Weak	Regionally restricted	4C	Expression is distal.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud mesenchyme	Strong	Graded	4D	Expression is strongest in distal cells underlying the apical ectodermal ridge.
TS15: forelimb bud ectoderm	Absent		4D

Structure	Level	Pattern	Image	Note
TS19: gonad	Present	Single cells	5A
TS19: genital tubercle	Present		5A
TS19: urinary system	Present	Regionally restricted	5A	Expression is in the utrethral epithelium.
TS19: hindgut epithelium	Present	Regionally restricted	5A	Expression is in the rectal epithelium.

Structure	Level	Pattern	Image	Note
TS19: gonad	Present	Single cells	5B

Structure	Level	Pattern	Image	Note
TS20: genital tubercle	Present	Regionally restricted	5C	Expression is towards the distal tip of the genital bud mesenchyme, reminiscent of that found in the limb buds.
TS20: urinary system	Present	Regionally restricted	5C	Expression is in the urethral epithelium.
TS20: hindgut epithelium	Present	Regionally restricted	5C	Expression is in the rectal epithelium.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	6A/B	Expression is in two thin symmetrical cell layers near the dorsal margin of the spinal cord.
TS19: genital tubercle	Present		6A/B
TS19: telencephalon	Present	Regionally restricted	6A/B	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Regionally restricted	6D/E	Expression is in two thin symmetrical cell layers near the dorsal margin of the spinal cord.
TS19: genital tubercle	Present		6D/E
TS19: telencephalon	Present	Regionally restricted	6D/E	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS19: myelencephalon alar plate	Present	Regionally restricted	6G/H	Expression is within two columns of cells.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	6J/K	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS19: cranial ganglion	Absent

Structure	Level	Pattern	Image	Note
TS20: limb	Absent

Structure	Level	Pattern	Image	Note
TS21: limb	Absent

Structure	Level	Pattern	Image	Note
TS22: seminiferous cord	Weak

Structure	Level	Pattern	Image	Note
TS23: seminiferous cord	Weak

Structure	Level	Pattern	Image	Note
TS22: ovary	Absent

Structure	Level	Pattern	Image	Note
TS23: ovary	Absent