Gene Expression Data
Assay Details
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Assay
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Reference:
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J:65601
Garel S, et al., Control of the migratory pathway of facial branchiomotor neurones. Development. 2000 Dec;127(24):5297-307
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1929073
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Gene symbol:
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Cntn2
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Gene name:
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contactin 2
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Probe:
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TAG-1 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1C:
embryonic day 11.5
(more  )
Specimen
1C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: metencephalon |
Present |
Regionally restricted |
1C
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Expression is in the migrating fbm neurons in the region of rhombomere 4,5 and 6.
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Specimen
2C:
embryonic day 11.5
(more )
Specimen
2C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: metencephalon |
Present |
Regionally restricted |
2C
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Expression is in the migrating fbm neurons in the region of rhombomere 4,5 and 6.
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Specimen
1D:
embryonic day 11.5
(more )
Specimen
1D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: metencephalon |
Present |
Regionally restricted |
1D
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Expression is in the fbm neurons.
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Specimen
2D:
embryonic day 12.5
(more )
Specimen
2D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: metencephalon |
Present |
Regionally restricted |
2D
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There is a high level of expression in fbm neurons migrating in the rhombomere 4 and 5 region, while the cells in rhombomere 6 have down-regulated the gene.
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Specimen
3B:
embryonic day 12.5; Mafbkr/Mafb +
(more )
Specimen
3B:
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Mafbkr/Mafb+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: metencephalon |
Present |
Regionally restricted |
3B
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Expression is in rhombomere regions 4 and 5. In rhombomere region 6, the level of expression is down-regulated in the cells that are migrating away from the dorsal midline.
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Specimen
3B':
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Mafbkr/Mafbkr
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: metencephalon |
Present |
Regionally restricted |
3B'
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Expression is in the migrating fbm neurons in the rhombomere 4 region. Rhombomere 5 is missing in this mutant and the expression is down-regulated in cells that are migrating away from the midline in the abnormal rhombomere 6.
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Specimen
4B:
embryonic day 12.5; Egr2tm1Pch/Egr2 +
(more )
Specimen
4B:
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: metencephalon |
Present |
Regionally restricted |
4B
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Expression is in migrating fbm neurons in rhombomere 4 and 5 regions. In the rhombomere 6 region, expression is down-regulated in cells migrating dorsally from the midline.
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Specimen
4B':
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6 * DBA/2
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Mutant Allele(s):
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Egr2tm1Pch/Egr2tm1Pch
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: metencephalon |
Present |
Regionally restricted |
4B'
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Expression is in migrating fbm neurons in rhombomere 4 and 5 regions. In the rhombomere 6 region, expression is down-regulated in cells migrating dorsally from the midline. Expression in this mutant is the same as in wild-type.
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Specimen
7A:
embryonic day 11.5
(more )
Specimen
7A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: metencephalon |
Moderate |
Regionally restricted |
7A
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Expression is in migrating fbm neurons in the rhombomere 4 and 5 region. The expression is down-regulated in cells that are migrating away from the midline in the rhombomere 6 region.
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Specimen
7A':
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Ebf1tm1Rug/Ebf1tm1Rug
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: metencephalon |
Weak |
Regionally restricted |
7A'
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In this mutant, expression is weaker in migrating cells than in wild type. Expression is in the migrating fbm neurons in rhombomere regions 4 and 5. Expression is down-regulated in the cells migrating from the midline in the rhombomere 6 region.
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Specimen
7C:
embryonic day 12.5
(more )
Specimen
7C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: metencephalon |
Present |
Regionally restricted |
7C
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Expression is in the migrating fbm neurons in the rhombomere 4 and 5 region. Expression is down-regulated as the cells migrate from the midline in the rhombomere 6 region.
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Specimen
7C':
(close )
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Genetic Background:
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involves: 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Ebf1tm1Rug/Ebf1tm1Rug
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: metencephalon |
Present |
Regionally restricted |
7C'
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In this mutant the gene is down-regulated prematurely in cells migrating out of the rhombomere 4 region.
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Analysis Tools