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Gene Expression Data
Assay Details
Assay
Reference: J:30345 Haramis AG, et al., The limb deformity mutation disrupts the SHH/FGF-4 feedback loop and regulation of 5' HoxD genes during limb pattern formation. Development. 1995 Dec;121(12):4237-45
Assay type: RNA in situ
MGI Accession ID: MGI:1335996
Gene symbol: Hoxd12
Gene name: homeobox D12
Probe: Hox5-6
Probe preparation: Antisense labelled with alkaline phosphatase RNA
Visualized with: Alkaline phosphatase
Results
Specimen 1A: embryonic day 10.25; Fmn1ld-Is(17;In2)1Gso/Fmn1+ (more )
Note: 31 somites.
Structure Level Pattern Image Note
TS16: forelimb bud mesenchyme Present Regionally restricted 1A Expression is proximal and posterior. Authors note similar results for hindlimbs.
TS16: tail Present 1A
Specimen 1B: embryonic day 10.25; Fmn1ld-Is(17;In2)1Gso/Fmn1ld-Is(17;In2)1Gso (more )
Note: 32 somites.
Structure Level Pattern Image Note
TS16: forelimb bud mesenchyme Present Regionally restricted 1B Expression is proximal and posterior. Expression in this mutant is the same as in wild-type embryos. Authors note similar results for hindlimbs.
TS16: tail Present Regionally restricted 1B
Specimen 1C: embryonic day 10.5; Fmn1ld-Is(17;In2)1Gso/Fmn1+ (more )
Note: 34 somites.
Structure Level Pattern Image Note
TS17: tail Present 1C
TS17: forelimb bud mesenchyme Present Regionally restricted 1C The proximal and posterior expression begins to expand anteriorly at this stage. Authors note similar results for hindlimbs.
Specimen 1D: embryonic day 10.5; Fmn1ld-Is(17;In2)1Gso/Fmn1ld-Is(17;In2)1Gso (more )
Note: 34 somites.
Structure Level Pattern Image Note
TS17: tail Present 1D
TS17: forelimb bud mesenchyme Present Regionally restricted 1D The up-regulation and anterior expansion of the expression domain seen in wild-type embryos is delayed in mutant embryos. Authors note similar results for hindlimbs.
Specimen 1E: embryonic day 10.75; Fmn1ld-Is(17;In2)1Gso/Fmn1+ (more )
Note: 38 somites.
Structure Level Pattern Image Note
TS17: tail Present 1E
TS17: forelimb bud mesenchyme Present Regionally restricted 1E The proximal, posterior expression begins to expand anteriorly and distally at this stage. Authors note similar results for hindlimbs.
Specimen 1F: embryonic day 10.75; Fmn1ld-Is(17;In2)1Gso/Fmn1ld-Is(17;In2)1Gso (more )
Note: 38 somites.
Structure Level Pattern Image Note
TS17: tail Present 1F
TS17: forelimb bud mesenchyme Weak Regionally restricted 1F Expression remains low and posteriorly restricted. Authors note similar results for hindlimbs.
Specimen 1G: embryonic day 12.0; Fmn1ld-Is(17;In2)1Gso/Fmn1+ (more )
Structure Level Pattern Image Note
TS20: forelimb Present Regionally restricted 1G Expression expands anteriorly in the distal compartment of the limb. Authors note similar results for hindlimbs.
Specimen 1H: embryonic day 12.0; Fmn1ld-Is(17;In2)1Gso/Fmn1ld-Is(17;In2)1Gso (more )
Structure Level Pattern Image Note
TS20: forelimb Present Regionally restricted 1H Expression in this mutant is the same as in wild-type embryos. Although delayed, upregulation of expression occurs anteriorly in the distal compartment. Authors note similar results for hindlimbs.
Specimen 2B: embryonic day 11.0; Fmn1ld-Is(17;In2)1Gso/Fmn1+ (more )
Note: 40 somites.
Structure Level Pattern Image Note
TS18: forelimb bud Present Regionally restricted 2B Expression is expanding anteriorly from the posterior domain.
Specimen 2E: embryonic day 11.0; Fmn1ld-Is(17;In2)1Gso/Fmn1ld-Is(17;In2)1Gso (more )
Note: 40 somites.
Structure Level Pattern Image Note
TS18: forelimb bud Present Regionally restricted 2E The anterior expansion of the expression domain is delayed in this mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/30/2025
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