Gene Expression Data
Assay Details
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Assay
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Reference:
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J:30341
Sucov HM, et al., Mouse embryos lacking RXR alpha are resistant to retinoic-acid-induced limb defects. Development. 1995 Dec;121(12):3997-4003
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1335048
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh cDNA8
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3I:
embryonic day 10.5
(more  )
Specimen
3I:
(close  )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Absent |
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3I
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Expression is not seen in the hindlimb until several hours after this stage.
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| TS17: forelimb bud |
Present |
Regionally restricted |
3I
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Expression is in the posterior-distal region.
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Specimen
3J:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Mutant Allele(s):
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Rxratm1Rev/Rxratm1Rev
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Absent |
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3J
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Expression in this mutant is the same as in wild-type embryos.
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| TS17: forelimb bud |
Present |
Regionally restricted |
3J
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Expression in this mutant is the same as in wild-type embryos. Expression is in the posterior-distal region of the limb bud.
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Specimen
3K:
embryonic day 11.5
(more )
Specimen
3K:
(close )
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Genetic Background:
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involves: 129S4/SvJae * C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
3K
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Expression is in the posterior-distal region of the limb bud.
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| TS19: hindlimb bud |
Present |
Regionally restricted |
3K
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Expression is in the posterior-distal region of the limb bud.
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Analysis Tools
