Gene Expression Data
Assay Details
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Assay
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Reference:
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J:52600
Yamaguchi TP, et al., A Wnt5a pathway underlies outgrowth of multiple structures in the vertebrate embryo. Development. 1999 Mar;126(6):1211-23
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1330058
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Gene symbol:
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Fgf8
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Gene name:
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fibroblast growth factor 8
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Probe:
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Fgf8 probe5
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
5a:
embryonic day 8.5
(more  )
Specimen
5a:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5b:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Wnt5atm1Amc/Wnt5atm1Amc
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5s:
embryonic day 10.5
(more )
Specimen
5s:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindlimb bud |
Present |
Regionally restricted |
5s
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Expression is in the apical ectodermal ridge.
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| TS17: genitourinary system |
Present |
Regionally restricted |
5s
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Expression is in the distal tip of the genital tubercle.
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Specimen
7a +/+:
embryonic day 11.5
(more )
Specimen
7a +/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
7a lower
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Expression is in the apical ectodermal ridge.
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Specimen
7a -/-:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Wnt5atm1Amc/Wnt5atm1Amc
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
7a upper
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Expression is in the apical ectoderma ridge. Expression is the same as in wild-type embryos.
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Specimen
7e +/+:
embryonic day 12.5
(more )
Specimen
7e +/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb |
Present |
Regionally restricted |
7e lower
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Expression is in the apical ectodermal ridge.
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Specimen
7e -/-:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Wnt5atm1Amc/Wnt5atm1Amc
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb |
Present |
Regionally restricted |
7e upper
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Expression is in the apical ectoderma ridge. Expression is the same as in wild-type embryos.
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Specimen
5i:
embryonic day 10.5
(more )
Specimen
5i:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: tail |
Present |
Regionally restricted |
5i
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Expression is in the anterior and posterior compartments of the myotome.
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| TS17: tail somite |
Present |
Regionally restricted |
5i
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Expression is in the anterior and posterior compartments of the myotome.
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Specimen
5j:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Wnt5atm1Amc/Wnt5atm1Amc
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: tail |
Present |
Regionally restricted |
5j
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Expression is in the anterior and posterior compartments of the myotome. The somites are shortened in mutant embryos.
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| TS17: tail somite |
Present |
Regionally restricted |
5j
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Expression is in the anterior and posterior compartments of the myotome. The somites are shortened in mutant embryos.
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Specimen
5t:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Wnt5atm1Amc/Wnt5atm1Amc
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: embryo |
Present |
Regionally restricted |
5t
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Expression is in a spot in the midline between the apical ectodermal ridge expression.
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Analysis Tools