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Gene Expression Data
Assay Details
Assay
Reference: J:46439 Freeman TC, et al., Expression Mapping of Mouse Genes. MGI Direct Data Submission. 1998;
Assay type: RT-PCR
MGI Accession ID: MGI:1204283
Gene symbol: Map3k1
Gene name: mitogen-activated protein kinase kinase kinase 1
Probe: st96_108
Visualized with: Ethidium bromide
Assay notes: MgCl2: 3.5 mM; Annealing temp: 55C; Annealing time: 90sec; Cycles: x40
Results
Image: st96_108 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 185 bp Amount Genetic BackgroundMutant Allele(s)Sex Note
Lane 1 P w 6-8 TS28: brain Moderate 0.01 µg; total RNA C57BL/6J Pooled
Lane 2 P w 6-8 TS28: cerebral cortex Moderate 0.01 µg; total RNA C57BL/6J Pooled
Lane 3 P w 6-8 TS28: hippocampus Moderate 0.01 µg; total RNA C57BL/6J Pooled
Lane 4 P w 6-8 TS28: midbrain Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 5 P w 6-8 TS28: corpus striatum Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 6 P w 6-8 TS28: main olfactory bulb Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 7 P w 6-8 TS28: cerebellum Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 8 P w 6-8 TS28: spinal cord Moderate 0.01 µg; total RNA C57BL/6J Pooled
Lane 9 P w 6-8 TS28: pituitary gland Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 10 P w 6-8 TS28: eye Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 11 P w 6-8 TS28: skeletal muscle Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 12 P w 6-8 TS28: heart Moderate 0.01 µg; total RNA C57BL/6J Pooled
Lane 13 P w 6-8 TS28: lung Moderate 0.01 µg; total RNA C57BL/6J Pooled
Lane 14 P w 6-8 TS28: spleen Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 15 P w 6-8 TS28: liver Moderate 0.01 µg; total RNA C57BL/6J Pooled
Lane 16 P w 6-8 TS28: gallbladder Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 17 P w 6-8 TS28: pancreas Moderate 0.01 µg; total RNA C57BL/6J Pooled
Lane 18 P w 6-8 TS28: metanephros Moderate 0.01 µg; total RNA C57BL/6J Pooled
Lane 19 P w 6-8 TS28: bladder Moderate 0.01 µg; total RNA C57BL/6J Pooled
Lane 20 P w 6-8 TS28: adrenal gland Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 21 P w 6-8 TS28: skin Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 22 P w 6-8 TS28: adipose tissue Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 23 P w 6-8 TS28: femur Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 24 P w 6-8 TS28: hand Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 25 P w 6-8 TS28: incisor Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 26 P w 6-8 TS28: tongue Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 27 P w 6-8 TS28: salivary gland Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 28 P w 6-8 TS28: esophagus Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 29 P w 6-8 TS28: stomach fundus Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 30 P w 6-8 TS28: pyloric antrum Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 31 P w 6-8 TS28: duodenum Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 32 P w 6-8 TS28: jejunum Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 33 P w 6-8 TS28: ileum Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 34 P w 6-8 TS28: cecum Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 35 P w 6-8 TS28: ascending colon Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 36 P w 6-8 TS28: descending colon Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 37 P w 6-8 TS28: apex of cecum Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 38 P w 6-8 TS28: thymus Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 39 P w 6-8 TS28: mammary gland Strong 0.01 µg; total RNA C57BL/6J Female Non-pregnant
Lane 40 P w 6-8 TS28: mammary gland Strong 0.01 µg; total RNA C57BL/6J Female Pregnant
Lane 41 P w 6-8 TS28: testis Strong 0.01 µg; total RNA C57BL/6J Male
Lane 42 P w 6-8 TS28: epididymis Strong 0.01 µg; total RNA C57BL/6J Male
Lane 43 P w 6-8 TS28: ovary Strong 0.01 µg; total RNA C57BL/6J Female
Lane 44 P w 6-8 TS28: cervix uteri Strong 0.01 µg; total RNA C57BL/6J Female
Lane 45 P w 6-8 TS28: placenta Strong 0.01 µg; total RNA C57BL/6J Female
Lane 46 E15-16 TS23: embryo Strong 0.01 µg; total RNA C57BL/6J Pooled
Lane 47 Control
Lane 48 Control

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/14/2024
MGI 6.23
The Jackson Laboratory