About   Help   FAQ
Phenotypes Associated with This Genotype
Genotype
MGI:5792659
Allelic
Composition
Ifngtm1.1Hayg/Ifngtm1.1Hayg
Genetic
Background
Not Specified
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ifngtm1.1Hayg mutation (1 available); any Ifng mutation (47 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
endocrine/exocrine glands
• thymus shows structural damage and atrophy

hematopoietic system
• thymus shows structural damage and atrophy
• immature erythrocytes fail to differentiate into mature cells, resulting in a severe 9-fold decrease in the total erythroid cell output
• treatment of 3 week old mice with an IFN-gamma-neutralizing antibody restores erythropoiesis
• bone marrow transplantation from wild-type mice into mutant mice resolves the blocks in erythropoiesis
• disruption in early B-cell differentiation, with a reduction in pre-B cells
• mice develop aplastic anemia
• bone marrow transplantation from wild-type mice into mutant mice reverses the aplastic anemia phenotype
• differentiation of multipotent progenitors to myeloid progenitors and differentiation of red blood cells and B cells is inhibited
• total number of burst-forming unit erythroid, colony-forming unit-granulocyte, -erythrocyte, -macrophage, and megakaryocyte, CFU-granulocyte, -macrophage, -megakaryocyte, and granulocyte progenitors are lower
• 4-fold decrease in the numbers of common myeloid progenitors
• bone marrow cellularity is further decreased with loss of myeloid cells at week 6
• 3-fold reduction in the total number of myeloid cells in the bone marrow
• total bone marrow cellularity is about 25% that of wild-type mice
• 4-fold decrease in the numbers of granulocyte/monocyte progenitors (GMP)
• bone marrow shows a decrease in megakaryocytes at 3 weeks of age
• total number of burst-forming unit erythroid number is lower
• 2-fold increase in the percentage of proerythorblasts in the bone marrow
• 2-fold increase in the percentage of polychromatophilic erythroblasts in the bone marrow
• 2-fold decrease in the percentage of orthochromatophilic erythroblasts in the bone marrow
• low red blood cell counts
• 2-fold decrease in the percentage of mature erythrocytes in the bone marrow
• 2-fold increase in the percentage of immature erythrocytes in the bone marrow
• low platelet counts
• treatment of 3 week old mice with an IFN-gamma-neutralizing antibody restores platelet levels
• slight expansion in four TCR Vbeta subfamilies in CD4 T cells (27%) and two Vbeta subfamilies in CD8 T cells (17%)
• low white blood cell counts
• the absolute cell numbers of pre-B cells and mature B cells is reduced about 8-fold
• 2-fold reduction in the percentage of pre-B cells, indicating a disruption in early B-cell differentiation
• the absolute cell numbers of pre-B and mature B cells is reduced about 8-fold
• peripheral blood pancytopenia
• proliferation of the LSK population is 2-fold higher than in wild-type mice
• proliferation of the KL population is 30% lower than in wild-type mice
• bone marrow transplantation from wild-type mice into mutant mice restores the hematopoietic progenitor and stem cell composition
• treatment of 3 week old mice with an IFN-gamma-neutralizing antibody reverses hematopoietic progenitor and stem cell composition
• 2.5-fold increase in long-term hematopoietic stem cells
• however, no differences in the numbers of short-term hematopoietic stem cells, multipotent progenitors, and common lymphoid progenitors are seen
• 8-fold decrease in the numbers of megakaryocyte/erythrocyte progenitors (MEPs)
• spleen shows structural damage and atrophy
• spleen and lymph node T cells show hyporesponsiveness after a mixed lymphocyte reaction
• bone marrow T cells produce lower cytokine levels after TCR stimulation

homeostasis/metabolism
• cytokine levels (IL-10, IL-17a, IFN-gamma, IL-4, IL-2, and IL-6) expressed by bone marrow T cells after TCR stimulation are lower
• serum levels of hematopoietic cytokines, Flt3 ligand, stem cell factor, IL-2, and erythropoietin are increased 2-, 1.5-, 3-, and 7-fold, respectively

immune system
• thymus shows structural damage and atrophy
• disruption in early B-cell differentiation, with a reduction in pre-B cells
• slight expansion in four TCR Vbeta subfamilies in CD4 T cells (27%) and two Vbeta subfamilies in CD8 T cells (17%)
• low white blood cell counts
• the absolute cell numbers of pre-B cells and mature B cells is reduced about 8-fold
• 2-fold reduction in the percentage of pre-B cells, indicating a disruption in early B-cell differentiation
• the absolute cell numbers of pre-B and mature B cells is reduced about 8-fold
• spleen shows structural damage and atrophy
• spleen and lymph node T cells show hyporesponsiveness after a mixed lymphocyte reaction
• bone marrow T cells produce lower cytokine levels after TCR stimulation
• cytokine levels (IL-10, IL-17a, IFN-gamma, IL-4, IL-2, and IL-6) expressed by bone marrow T cells after TCR stimulation are lower
• serum levels of hematopoietic cytokines, Flt3 ligand, stem cell factor, IL-2, and erythropoietin are increased 2-, 1.5-, 3-, and 7-fold, respectively
• lymph nodes show structural damage and atrophy

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
aplastic anemia DOID:12449 OMIM:609135
J:220784


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
03/19/2024
MGI 6.23
The Jackson Laboratory