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Phenotypes Associated with This Genotype
Genotype
MGI:3606141
Allelic
Composition
Enpp1ttw/Enpp1ttw
Genetic
Background
involves: ICR
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Enpp1ttw mutation (0 available); any Enpp1 mutation (9 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• start to die around 18 weeks of age with most lost before 30 weeks of age

cellular
• disorganized proliferation of chondrocytes in the protruded vertebral disks

skeleton
• calcification of the Achilles tendon
• changes are more pronounced and occur earlier in the superior spine than in the inferior spine (J:19124)
• at 8 weeks of age, rigidity of the vertebral column (J:37369)
• proliferation of cartilaginous tissues followed by endochondral ossification occurs around the site of tendon attachment and the around the osseous membrane (J:19124)
• at 10 weeks, proliferation of small fibroblast-like cells is seen at the site of ligament attachment and the posterior corner of the endplate (J:43357)
• moderate
• at 6 - 8 weeks , degeneration and necrosis of the chondrocytes with surrounding tidemarks of calcification are seen in the articular cartilage
• treatment with streptozotocin increased membranous and enchondral ossification of the posterior longitudinal ligament of the cervical spine, esp. in the area of the ligamentous enthesis
• treatment with streptozotocin also increased the number of alkaline phosphatase positive osteoblast-like mesenchymal cells esp. around the enthesis
• at 3 - 4 weeks, papillary proliferation of synovial cells protrude into the articular space (J:19124)
• at 6 - 8 weeks, cystic dilation of the articular sac, proliferation of synovial cells replacing articular cartilage, and some thicker collagen fibers with a longer periodicity are seen (J:19124)
• at 8 - 12 weeks, fibrosis of the synovial tissue is seen, proliferation is not as obvious, and the articular sac is fibrous and thickened with calcification and proliferation of the cartilaginous tissue (J:19124)
• around the site of tendon attachment osteophytes develop and after 20 weeks of age these may bridge the joint (J:19124)
• at 8 weeks of age, stiffness of limb joints (J:37369)
• initially at 4 weeks of age, an amorphous calcifying lesion is detected protruding from the cartilaginous endplates into the fibrocartilage in the superior and inferior spine (J:19124)
• this lesion increases in size with age and degenerating or necrotic chondrocytes are seen around the lesion (J:19124)
• abnormal disks protrude into the spinal canaliculi or surrounding soft tissue and are associated with thickening of the osseous membranes and bone formation outside the degenerated calcified fibrous cartilage (J:19124)
• degeneration and destruction of the cartilage matrix with lateral protrusion (J:37369)
• disorganized proliferation of chondrocytes and irregular calcification in the protruded vertebral disks (J:37369)
• progressive dorsal and posterior protrusion of amorphous cartilaginous tissue from the posterior part of the intervertebral disk (J:43357)
• osteoblast-like cells are detected in the posterior intervertebral disks and the membrane surrounding the amorphous protruding tissue is progressively ossified (J:43357)
• deposition of calcium in the intervertebral spaces especially at the edges of the vertebral bodies
• calcified deposits can connect adjacent vertebral bodies similar to spur formation in osteoarthritis
• proliferation of fibrous tissue of the periosteum in areas over protruding intervertebral disks
• at 4 weeks of age calcium deposits begin to accumulate (J:19124)
• at 6 - 8 weeks, calcium deposits are seen in the articular space and synovial tissue by 20 weeks of age the peripheral articular cartilage is replaced by bony tissue (J:19124)
• by 20 weeks of age the peripheral articular cartilage is replaced by bony tissue (J:19124)
• no pronounced deposition of calcium is seen in the kidneys (J:19124)
• in limb joints (J:37369)
• fibrous thickening of the ligaments around protruding intervertebral disks
• hypertrophy of the posterior longitudinal ligament in the cervical spine at 8 weeks of age
• at 10 weeks, proliferation of small fibroblast-like cells is seen at the site of ligament attachment in the spine
• osteoblast-like cells are detected in the posterior longitudinal ligament
• treatment with streptozotocin increases the area of ossification of the posterior longitudinal ligament
• disorganized proliferation of chondrocytes in the protruded vertebral disks
• erosion and destruction of limb joint articular cartilage with synovial cell proliferation but only occasional inflammation (J:37369)
• subchondral bone absorption beneath the joint surface (J:37369)
• deposition of calcium in the intervertebral spaces especially at the edges of the vertebral bodies (J:37369)
• irregular calcium deposits in areas over protruding intervertebral disks (J:37369)
• ossification of the membrane surrounding the abnormal amorphous tissue of the ligament enthesis (J:43357)

growth/size/body
• streptozotocin-treated mice do not show any increase in body weight compared to to untreated mice unlike in wild-type mice
• at 8 weeks of age, cease to gain weight

behavior/neurological
• impaired grasping ability at 3 - 4 weeks of age
• by 8 weeks of age mice appear to walk on tiptoes

cardiovascular system
• calcification of the aorta

homeostasis/metabolism
N
• serum calcium and phosphorus levels are similar to wild-type
• calcification of the hair follicles of the vibrissae, aorta, Achilles tendon, and limb joints
• serum alkaline phosphatase activity is increased at all ages tested and increases rather than decreases with age

immune system
• erosion and destruction of limb joint articular cartilage with synovial cell proliferation but only occasional inflammation (J:37369)
• subchondral bone absorption beneath the joint surface (J:37369)

muscle
• calcification of the Achilles tendon


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/21/2024
MGI 6.23
The Jackson Laboratory