Analysis Tools
digestive/alimentary system
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• intestine of mice treated with tamoxifen between 25 and 40 days of age for a total of 8 days and a minimum of 4-week washout period exhibits thickened smooth muscle layers; an increase in both circular and longitudinal jejunum smooth muscle thickness and in longitudinal colonic muscle thickness are seen
• however, no histopatholgical changes are seen in the intestine, with no infiltration of inflammatory cells or atrophy of villi and cell size in TAM treated mice
• structure of smooth muscle appears normal in TAM treated mice suggesting that hypertrophy is not causative of the muscle thickening and markers of smooth muscle phenotypic modulation do not indicate cell dedifferentiation
• Ccnd1, but not Pcna, is upregulated in TAM treated mice, suggesting that smooth muscle cells may enter but not progress through the cell cycle or could indicate cellular senescence
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• whole intestines are shorter in TAM treated mice
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• small intestine length is shorter while colon length is unaffected in TAM treated mice
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• mice treated with tamoxifen exhibit a delay in both small intestine and colonic transit
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growth/size/body
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• small weight increase and no change in body length is seen 2 months after TAM treatment
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muscle
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• intestine of mice treated with tamoxifen between 25 and 40 days of age for a total of 8 days and a minimum of 4-week washout period exhibits thickened smooth muscle layers; an increase in both circular and longitudinal jejunum smooth muscle thickness and in longitudinal colonic muscle thickness are seen
• however, no histopatholgical changes are seen in the intestine, with no infiltration of inflammatory cells or atrophy of villi and cell size in TAM treated mice
• structure of smooth muscle appears normal in TAM treated mice suggesting that hypertrophy is not causative of the muscle thickening and markers of smooth muscle phenotypic modulation do not indicate cell dedifferentiation
• Ccnd1, but not Pcna, is upregulated in TAM treated mice, suggesting that smooth muscle cells may enter but not progress through the cell cycle or could indicate cellular senescence
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