Mbnl2^em1.1Coop
Endonuclease-mediated Allele Detail

Summary

• Symbol: Mbnl2^em1.1Coop
• Name: muscleblind like splicing factor 2; endonuclease-mediated mutation 1.1, Thomas A Cooper
• MGI ID: MGI:8350478
• Gene: Mbnl2 Location: Chr14:120513081-120669109 bp, + strand Genetic Position: Chr14, 64.44 cM
• Alliance: Mbnl2^em1.1Coop page

Mutation origin

• Strain of Origin: C57BL/6NJ

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 2 that contains the translational start site was flanked by loxP sites via CRISPR/Cas9 mediated recombination. Preimplantation embryos incubated with cell-permeable cre recombinase (HTNCre) resulted in site-specific recombination and deletion of exon 2 and germline transmission was established. (J:386516)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mbnl2 Mutation: 65 strains or lines available

References

• Original: J:386516 Peterson JAM, et al., MBNL loss of function in smooth muscle as a model for myotonic dystrophy associated gastrointestinal dysmotility. Proc Natl Acad Sci U S A. 2025 Dec 16;122(50):e2522788122
• All: 1 reference(s)