Phenotypes associated with this allele

• Allele Symbol: Iqsec3^{tm1c(KOMP)Wtsi}
• Allele Name: targeted mutation 1c, Wellcome Trust Sanger Institute
• Allele ID: MGI:8257180
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Iqsec3^tm1c(KOMP)Wtsi/Iqsec3^tm1c(KOMP)Wtsi Tg(Nes-cre)1Kln/0	B6.Cg-Iqsec3^tm1c(KOMP)Wtsi Tg(Nes-cre)1Kln	MGI:8257559

Genotype
MGI:8257559

cn1

• Allelic Composition: Iqsec3^{tm1c(KOMP)Wtsi}/Iqsec3^{tm1c(KOMP)Wtsi}; Tg(Nes-cre)1Kln/0
• Genetic Background: B6.Cg-Iqsec3^{tm1c(KOMP)Wtsi} Tg(Nes-cre)1Kln

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

behavior/neurological phenotype ( J:373367 )

N • mice show comparable locomotion, climbing, rearing, gooming, drinking, and eating activities, anxiety-like behaviors in the open field, light and dark transition, and elevated plus maze tests, and no changes in working memory or exploratory behavior in Y maze, novel object recognition, and Morris water maze, or in social interaction and social novelty recognition memory in the three-chamber test compared to wild-type mice

impaired contextual conditioning behavior ( J:373367 )

• context-dependent freezing elicited by the context 24 hours after training is reduced suggesting impaired long-term fear memory formation

• however, mice are able to acquire fear conditioning similarly to wild-type mice and show comparable context-dependent freezing elicited by the context 1 hour after training indicating normal short-term fear memory

decreased freezing behavior ( J:373367 )

• context-dependent freezing elicited by the context 24 hours after training is reduced

nervous system

abnormal synapse morphology ( J:373367 )

• density and area of GABAergic synaptic markers VGAT and GABA(A)Rgamma2 are reduced in all hippocampal CA1 layers and the number of GABA+ presynaptic boutons in the stratum radiatum layer of hippocampal CA1 regions is decreased, indicating a decrease in the number of GABAergic synapses

• however, density and size of VGLUT1 puncta are unchanged

decreased inhibitory postsynaptic current amplitude ( J:373367 )

• the amplitude of somatic IPSCs evoked by stimulation of axons in the stratum pyramidale layer is reduced by approximately 51.3%

• the amplitude of dendritic IPSCs evoked by stimulation of axons in the startum lacunosum moleculare layer is reduced by approximately 65.6%

reduced long-term potentiation ( J:373367 )

• NMDAR-dependent long-term potentiation (LTP) at Schaffer collateral-to-CA1 synapses induced by theta burst stimulation at 100 Hz is modestly, but significantly, reduced, with no alterations in fiber volley size, paired-pulse facilitation of field excitatory postsynaptic potentials, or NMDAR-dependent long-term depression

decreased miniature inhibitory postsynaptic current frequency ( J:373367 )

• the frequency, but not the amplitude, of miniature inhibitory postsynaptic currents (mIPSCs) are decreased in hippocampal CA1 pyramidal neurons

• however, no alterations in the frequency or amplitude of miniature excitatory postsynaptic currents (mEPSCs) are seen in hippocampal CA1 pyramidal neurons