Analysis Tools
reproductive system
 N |
• 11- to 15-week-old female heterozygotes are fertile and show normal folliculogenesis with presence of early and secondary follicles, antral follicles, and multiple corpus lutea
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azoospermia
(
J:324156
)
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• 14- to 15-week-old males show azoospermia
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• seminiferous tubules lack postmeiotic elongated spermatids at 9 weeks of age
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• seminiferous tubules lack postmeiotic round spermatids at 9 weeks of age
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• seminiferous tubules from 9-week-old males contain meiotic spermatocytes but lack postmeiotic round and elongated spermatids
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• early prophase I stages (leptonema/zygonema) are present but meiotic prophase I progression is blocked during late pachynema and/or diplonema
• no cells containing abnormally condensed metaphase-like chromosomes are detected in adult males
• pH3 staining shows that luminally located metaphase (pH3-positive) cells are absent or extremely rare from 14 dpp to 24 dpp and at later ages, indicating the lack of a premature metaphase entry phenotype
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• TUNEL staining shows a significant increase in the % of tubules containing 10 or more apoptotic cells at 16 dpp and later ages, including adulthood
• however, no rampant apoptosis is detected during early ages (8, 10, and 14 dpp)
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• from 16 dpp onward, fewer seminiferous tubules contain one layer of cells in late pachynema/diplonema plus a new layer in leptonema/zygonema (1% at 16 dpp vs 18% in wild-type controls); when present, these tubules have significantly fewer cells in the late pachytene/diplotene layer
• pachytene cells are depleted in some tubules with a single pachytene layer while abnormal tubules also contain dying cells with fragmented and intense SYCP3 staining from 16 dpp onward
• tubules containing round or elongating spermatids are absent
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• adult testis weight-to-body weight ratios are 33.5% smaller than in littermate controls
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• 14- to 15-week-old males show testicular atrophy
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• male heterozygotes are sterile; none of 7 males tested sire offspring when bred with wild-type females for >14 weeks
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endocrine/exocrine glands
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• from 16 dpp onward, fewer seminiferous tubules contain one layer of cells in late pachynema/diplonema plus a new layer in leptonema/zygonema (1% at 16 dpp vs 18% in wild-type controls); when present, these tubules have significantly fewer cells in the late pachytene/diplotene layer
• pachytene cells are depleted in some tubules with a single pachytene layer while abnormal tubules also contain dying cells with fragmented and intense SYCP3 staining from 16 dpp onward
• tubules containing round or elongating spermatids are absent
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• adult testis weight-to-body weight ratios are 33.5% smaller than in littermate controls
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• 14- to 15-week-old males show testicular atrophy
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cellular
azoospermia
(
J:324156
)
|
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• 14- to 15-week-old males show azoospermia
|
|
|
• seminiferous tubules lack postmeiotic elongated spermatids at 9 weeks of age
|
|
|
• seminiferous tubules lack postmeiotic round spermatids at 9 weeks of age
|
|
|
• seminiferous tubules from 9-week-old males contain meiotic spermatocytes but lack postmeiotic round and elongated spermatids
|
|
|
• early prophase I stages (leptonema/zygonema) are present but meiotic prophase I progression is blocked during late pachynema and/or diplonema
• no cells containing abnormally condensed metaphase-like chromosomes are detected in adult males
• pH3 staining shows that luminally located metaphase (pH3-positive) cells are absent or extremely rare from 14 dpp to 24 dpp and at later ages, indicating the lack of a premature metaphase entry phenotype
|
|
|
• TUNEL staining shows a significant increase in the % of tubules containing 10 or more apoptotic cells at 16 dpp and later ages, including adulthood
• however, no rampant apoptosis is detected during early ages (8, 10, and 14 dpp)
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