Ythdc2^em3Sky
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ythdc2^em3Sky
• Name: YTH domain containing 2; endonuclease-mediated mutation 3, Scott Keeney
• MGI ID: MGI:8253161
• Synonyms: Ythdc2^EQ
• Gene: Ythdc2 Location: Chr18:44961521-45022782 bp, + strand Genetic Position: Chr18, 23.81 cM
• Alliance: Ythdc2^em3Sky page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence)
• Mutation: Single point mutation
• Mutation details: TALEN-targeting of exon 7 introduced a single point mutation (GAA to CAG) within motif II in the helicase DEXDc module of YTHDC2, leading to the amino acid substitution of glutamate with glutamine at position 332 (E332Q). This mutation is expected to maintain ATP binding while disrupting hydrolysis. The equivalent mutation in human YTHDC2 has been shown to abolish ATP hydrolysis and helicase activity in vitro. (J:324156)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ythdc2 Mutation: 58 strains or lines available

References

• Original: J:324156 Saito Y, et al., YTHDC2 control of gametogenesis requires helicase activity but not m(6)A binding. Genes Dev. 2022 Feb 1;36(3-4):180-194
• All: 1 reference(s)