Phenotypes associated with this allele

• Allele Symbol: Sass6^em1Baz
• Allele Name: endonuclease-mediated mutation 1, Hisham Bazzi
• Allele ID: MGI:8242359
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Sass6^em1Baz/Sass6^em1Baz	involves: FVB/NRj	MGI:8242361
cx2	Sass6^em1Baz/Sass6^em1Baz Trp53^tm1Brd/Trp53^tm1Brd	involves: 129S7/SvEvBrd * FVB/NRj	MGI:8242363
cx3	Sass6^em1Baz/Sass6^em1Baz Tg(CAG-EGFP/CETN2)3-4Jgg/0	involves: BALB/c * C57BL/6 * FVB/NRj	MGI:8242377
cx4	Sass6^em1Baz/Sass6^em1Baz Trp53bp1^em1Baz/Trp53bp1^em1Baz	involves: FVB/NRj	MGI:8242364
cx5	Sass6^em1Baz/Sass6^em1Baz Usp28^em1Baz/Usp28^em1Baz	involves: FVB/NRj	MGI:8242365

Genotype
MGI:8242361

hm1

• Allelic Composition: Sass6^em1Baz/Sass6^em1Baz
• Genetic Background: involves: FVB/NRj

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality prior to organogenesis, complete penetrance ( J:347621 )

• arrest at ~E9.5 with heart formation but no somites

embryo

abnormal embryo turning ( J:347621 )

embryonic growth arrest ( J:347621 )

• arrest at ~E9.5

impaired somite development ( J:347621 )

• do not show somites at ~E9.5

cellular

abnormal microtubule organizing center morphology ( J:347621 )

• at E9.0, only rare and single centrioles are found in mitotic poles in 11% of cells compared to wild-type where each pole of a mitotic spindle contains a pair of centrioles

abnormal mitosis ( J:347621 )

• increase in the fraction of mitotic cells (10% compared to 5% in wild-type embryos)

Genotype
MGI:8242363

cx2

• Allelic Composition: Sass6^em1Baz/Sass6^em1Baz; Trp53^tm1Brd/Trp53^tm1Brd
• Genetic Background: involves: 129S7/SvEvBrd * FVB/NRj

embryo

embryo phenotype ( J:347621 )

N • restoration of embryo turning and somite formation at E9.5 compared to mutant embryos wild-type for Trp53

Genotype
MGI:8242377

cx3

• Allelic Composition: Sass6^em1Baz/Sass6^em1Baz; Tg(CAG-EGFP/CETN2)3-4Jgg/0
• Genetic Background: involves: BALB/c * C57BL/6 * FVB/NRj

cellular

abnormal microtubule organizing center morphology ( J:347621 )

• very few cells from E9.0 embryos show centrioles (6% compared to 98% in wild-type embryos)

Genotype
MGI:8242364

cx4

• Allelic Composition: Sass6^em1Baz/Sass6^em1Baz; Trp53bp1^em1Baz/Trp53bp1^em1Baz
• Genetic Background: involves: FVB/NRj

embryo

embryo phenotype ( J:347621 )

• restoration of development based on size and morphology at E9.5 compared to mutant embryos wild-type for Trp53bp1

Genotype
MGI:8242365

cx5

• Allelic Composition: Sass6^em1Baz/Sass6^em1Baz; Usp28^em1Baz/Usp28^em1Baz
• Genetic Background: involves: FVB/NRj

embryo

embryo phenotype ( J:347621 )

• restoration of development based on size and morphology at E9.5 compared to mutant embryos wild-type for Usp28