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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Lnx1tm1Henk
targeted mutation 1, Mark Henkemeyer
MGI:8228037
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Lnx1tm1Henk/Lnx1tm1Henk involves: 129S1/Sv * 129X1/SvJ * CD-1 MGI:8228038
cx2
 		Lnx1tm1Henk/Lnx1tm1Henk
Tg(Thy1-EGFP)MJrs/0 		involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * CBA * CD-1 MGI:8228048
cx3
 		Ephb2tm1Jf/Ephb2tm1Jf
Lnx1tm1Henk/Lnx1tm1Henk 		involves: 129S1/Sv * 129X1/SvJ * CD-1 MGI:8228079
cx4
 		Ephb1tm2Cmn/Ephb1tm2Cmn
Lnx1tm1Henk/Lnx1tm1Henk 		involves: 129S1/Sv * 129X1/SvJ * CD-1 MGI:8228080
cx5
 		Efnb3tm1.1Henk/Efnb3tm1.1Henk
Ephb2tm1Jf/Ephb2tm1Jf
Lnx1tm1Henk/Lnx1tm1Henk 		involves: 129S1/Sv * 129X1/SvJ * CD-1 MGI:8228084


Genotype
MGI:8228038
hm1
Allelic
Composition		 Lnx1tm1Henk/Lnx1tm1Henk
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * CD-1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lnx1tm1Henk mutation (0 available); any Lnx1 mutation (43 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
abnormal axon guidance ( J:267455 )
• decrease in the ratio of CA3 neuron spines that overlap ZnT3-labeled terminals compared to the total number of synapses
• in coculture, there is an increase in the rate of axons with type none and and a decrease in the rate of presynaptic type, but no change in the rates of postsynaptic or both types
• in coculture, newborn boutons show reduced expanding terminals when in contact with null spines compared to wild-type spines
• rapid terminal shrinkage and split of refined boutons is attenuated when cocultured with null neurons
abnormal axon pruning ( J:267455 )
• the infrapyramidal bundle (IPB) axons grow into split CA3 pyramidal layers and are not shortened during development, instead these remain long and stable length into adulthood indicating a defect in axon pruning and targeting
• cell co-culture experiments indicate non-cell-autonomous regulation of mossy fiber axon development likely in a manner of axon-cell/dendrite contact
abnormal hippocampus CA3 region morphology ( J:267455 )
• decrease in the ratio of CA3 neuron spines that overlap ZnT3-labeled terminals compared to the total number of synapses
• decreased cell density seen as a loose pattern in the CA3 cell layers
abnormal hippocampal mossy fiber morphology ( J:267455 )
• the infrapyramidal bundle (IPB) axons grow into split CA3 pyramidal layers and are not shortened during development, instead these remain long and stable length into adulthood indicating a defect in axon pruning and targeting
• dramatic increase in IPB axon terminals in contact with neurons within the CA3 pyramidal cell layer
• decrease in the ratio of mossy fiber axon terminal to CA3 spine contacts vs terminal to CA3 shaft contacts
abnormal synaptic bouton morphology ( J:267455 )
• in the CA3 region, there is a decrease in vesicle release sites and docked vesicles in the presynaptic active zone
• decrease in the correlation between vesicle number/distribution area and PSD length
abnormal asymmetric synapse morphology ( J:267455 )
• decrease in vesicle density in synaptic terminal and increase in the distance of vesicles to membrane in the CA3 region
abnormal postsynaptic density morphology ( J:267455 )
• in the CA3 region, there is a decrease in vesicle release sites
• decrease in the correlation between vesicle number/distribution area and PSD length in the CA3 region
increased paired-pulse ratio ( J:267455 )
• increased PPR following suprapyramidal bundle (SPB) or infrapyramidal bundle (IPB) stimulation at 3 weeks of age

cellular
abnormal axon guidance ( J:267455 )
• decrease in the ratio of CA3 neuron spines that overlap ZnT3-labeled terminals compared to the total number of synapses
• in coculture, there is an increase in the rate of axons with type none and and a decrease in the rate of presynaptic type, but no change in the rates of postsynaptic or both types
• in coculture, newborn boutons show reduced expanding terminals when in contact with null spines compared to wild-type spines
• rapid terminal shrinkage and split of refined boutons is attenuated when cocultured with null neurons
abnormal axon pruning ( J:267455 )
• the infrapyramidal bundle (IPB) axons grow into split CA3 pyramidal layers and are not shortened during development, instead these remain long and stable length into adulthood indicating a defect in axon pruning and targeting
• cell co-culture experiments indicate non-cell-autonomous regulation of mossy fiber axon development likely in a manner of axon-cell/dendrite contact




Genotype
MGI:8228048
cx2
Allelic
Composition		 Lnx1tm1Henk/Lnx1tm1Henk
Tg(Thy1-EGFP)MJrs/0
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * CBA * CD-1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lnx1tm1Henk mutation (0 available); any Lnx1 mutation (43 available)
Tg(Thy1-EGFP)MJrs mutation (2 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
abnormal hippocampus CA3 region morphology ( J:267455 )
• increase in spine density but decrease in mushroom-shaped spines of CA3 neurons at 3 weeks of age
abnormal dendritic mushroom spine morphology ( J:267455 )
• increase in spine density but decrease in mushroom-shaped spines of CA3 neurons at 3 weeks of age
increased dendritic spine density ( J:267455 )
• increase in spine density but decrease in mushroom-shaped spines of CA3 neurons at 3 weeks of age




Genotype
MGI:8228079
cx3
Allelic
Composition		 Ephb2tm1Jf/Ephb2tm1Jf
Lnx1tm1Henk/Lnx1tm1Henk
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * CD-1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ephb2tm1Jf mutation (0 available); any Ephb2 mutation (68 available)
Lnx1tm1Henk mutation (0 available); any Lnx1 mutation (43 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
nervous system phenotype ( J:267455 )
N
• rescue of mossy fiber axon terminal targeting compared to mice null for Lnx1 alone
• paired-pulse ratio is also restored to normal levels




Genotype
MGI:8228080
cx4
Allelic
Composition		 Ephb1tm2Cmn/Ephb1tm2Cmn
Lnx1tm1Henk/Lnx1tm1Henk
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * CD-1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ephb1tm2Cmn mutation (0 available); any Ephb1 mutation (44 available)
Lnx1tm1Henk mutation (0 available); any Lnx1 mutation (43 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
abnormal hippocampal mossy fiber morphology ( J:267455 )
• mossy fiber defects are similar to mice null for Lnx1 alone




Genotype
MGI:8228084
cx5
Allelic
Composition		 Efnb3tm1.1Henk/Efnb3tm1.1Henk
Ephb2tm1Jf/Ephb2tm1Jf
Lnx1tm1Henk/Lnx1tm1Henk
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * CD-1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Efnb3tm1.1Henk mutation (0 available); any Efnb3 mutation (11 available)
Ephb2tm1Jf mutation (0 available); any Ephb2 mutation (68 available)
Lnx1tm1Henk mutation (0 available); any Lnx1 mutation (43 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
abnormal hippocampal mossy fiber morphology ( J:267455 )
• disruption of mossy fiber axon terminal targeting




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
07/29/2025
MGI 6.24 		The Jackson Laboratory