Ephb2^tm1Jf
Targeted Allele Detail

Summary

• Symbol: Ephb2^tm1Jf
• Name: Eph receptor B2; targeted mutation 1, Jonas Frisen
• MGI ID: MGI:3692752
• Synonyms: Ephb2^F620D
• Gene: Ephb2 Location: Chr4:136374850-136563299 bp, - strand Genetic Position: Chr4, 69.0 cM, cytoband D-E
• Alliance: Ephb2^tm1Jf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:115873
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutation: Nucleotide substitutions
• Mutation details: A mutation was engineered in exon 10 (ENSMUST00000059287) to change phenylalanine codon 613 (TTT) to aspartic acid (GAT( (p.F613D) and a loxP site flanked neomycin resistance gene cassette was inserted into intron 9. The neo cassette was deleted through subsequent Cre-mediated recombination. (J:115873)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ephb2 Mutation: 66 strains or lines available

References

• Original: J:115873 Holmberg J, et al., EphB receptors coordinate migration and proliferation in the intestinal stem cell niche. Cell. 2006 Jun 16;125(6):1151-63
• All: 6 reference(s)