Phenotypes associated with this allele

• Allele Symbol: Rbpms^tm1.1Thbr
• Allele Name: targeted mutation 1.1, Thomas Braun
• Allele ID: MGI:8226420
• Summary: 7 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gt(ROSA)26Sor^tm11(CAG-tdTomato*,-GFP*)Nat/? Rbpms^tm1.1Thbr/Rbpms^tm1.1Thbr Rbpms2^tm2.1Thbr/Rbpms2^tm2.1Thbr Tg(Myh6-cre)2182Mds/0	involves: 129 * C57BL/6 * FVB/N	MGI:8226709
cn2	Gt(ROSA)26Sor^tm11(CAG-tdTomato*,-GFP*)Nat/? Rbpms^tm1.1Thbr/Rbpms^tm1.1Thbr Rbpms2^tm2.1Thbr/Rbpms2^tm2.1Thbr Tg(myl7.L-cre)1118Tmhn/0	involves: 129 * C57BL/6 * MF1	MGI:8226708
cn3	Nkx2-5^tm2(cre)Rph/Nkx2-5^+ Rbpms^tm1.1Thbr/Rbpms^tm1.1Thbr	involves: 129S1/Sv * 129S4/SvJae * C57BL/6	MGI:8226638
cn4	Rbpms^tm1.1Thbr/Rbpms^tm1.1Thbr Rbpms2^tm2.1Thbr/Rbpms2^tm2.1Thbr	involves: 129S4/SvJae * C57BL/6	MGI:8226711
cn5	Rbpms^tm1.1Thbr/Rbpms^tm1.1Thbr Rbpms2^tm2.1Thbr/Rbpms2^tm2.1Thbr Tg(Myh6-cre)2182Mds/0	involves: 129S4/SvJae * C57BL/6 * FVB/N	MGI:8226672
cn6	Rbpms^tm1.1Thbr/Rbpms^tm1.1Thbr Rbpms2^tm2.1Thbr/Rbpms2^tm2.1Thbr Tg(myl7.L-cre)1118Tmhn/0	involves: 129S4/SvJae * C57BL/6 * MF1	MGI:8226669
cn7	Rbpms^tm1.1Thbr/Rbpms^tm1.1Thbr Tg(myl7.L-cre)1118Tmhn/0	involves: 129S4/SvJae * C57BL/6 * MF1	MGI:8226627

Genotype
MGI:8226709

cn1

• Allelic Composition: Gt(ROSA)26Sor^{tm11(CAG-tdTomato*,-GFP*)Nat}/?; Rbpms^tm1.1Thbr/Rbpms^tm1.1Thbr; Rbpms2^tm2.1Thbr/Rbpms2^tm2.1Thbr; Tg(Myh6-cre)2182Mds/0
• Genetic Background: involves: 129 * C57BL/6 * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cardiovascular system

abnormal fetal cardiomyocyte morphology ( J:369747 )

• at E16.5 the nuclei are irregular in outline and size or associated with micronuclei

increased fetal cardiomyocyte apoptosis ( J:369747 )

• at E18.5

muscle

increased fetal cardiomyocyte apoptosis ( J:369747 )

• at E18.5

cellular

increased fetal cardiomyocyte apoptosis ( J:369747 )

• at E18.5

abnormal cell nucleus morphology ( J:369747 )

• at E16.5 the nuclei of cardiomyocytes are irregular in outline and size or associated with micronuclei

increased micronucleus incidence ( J:369747 )

• at E16.5 the nuclei of cardiomyocytes are irregular in outline and size or associated with micronuclei

Genotype
MGI:8226708

cn2

• Allelic Composition: Gt(ROSA)26Sor^{tm11(CAG-tdTomato*,-GFP*)Nat}/?; Rbpms^tm1.1Thbr/Rbpms^tm1.1Thbr; Rbpms2^tm2.1Thbr/Rbpms2^tm2.1Thbr; Tg(myl7.L-cre)1118Tmhn/0
• Genetic Background: involves: 129 * C57BL/6 * MF1

cardiovascular system

abnormal fetal cardiomyocyte morphology ( J:369747 )

• at E12.5 the nuclei are irregular in outline and size or associated with micronuclei

cellular

abnormal cell nucleus morphology ( J:369747 )

• at E12.5 the nuclei of cardiomyocytes are irregular in outline and size or associated with micronuclei

increased micronucleus incidence ( J:369747 )

• at E12.5 the nuclei of cardiomyocytes are irregular in outline and size or associated with micronuclei

Genotype
MGI:8226638

cn3

• Allelic Composition: Nkx2-5^tm2(cre)Rph/Nkx2-5⁺; Rbpms^tm1.1Thbr/Rbpms^tm1.1Thbr
• Genetic Background: involves: 129S1/Sv * 129S4/SvJae * C57BL/6

mortality/aging

premature death ( J:369747 )

• viable at birth but only ~25% are still alive at 40 weeks of age

cardiovascular system

small heart ( J:369747 )

• at 30 weeks of age

digestive/alimentary system

distended duodenum ( J:369747 )

• at 30 weeks of age

distended stomach ( J:369747 )

• at 30 weeks of age

hematopoietic system

small spleen ( J:369747 )

• at 30 weeks of age

immune system

small spleen ( J:369747 )

• at 30 weeks of age

Genotype
MGI:8226711

cn4

• Allelic Composition: Rbpms^tm1.1Thbr/Rbpms^tm1.1Thbr; Rbpms2^tm2.1Thbr/Rbpms2^tm2.1Thbr
• Genetic Background: involves: 129S4/SvJae * C57BL/6

cardiovascular system

abnormal fetal cardiomyocyte morphology ( J:369747 )

• following adeno-cre infection of isolated E14.5 cardiomyocytes, proliferating cardiomyocytes show a higher rate of nuclear defects compared to non-proliferating cells

• following adeno-cre infection of isolated E14.5 cardiomyocytes, spindle and chromosome distribution defects are seen with an increase in the percentage of cells with unaligned chromosomes, multi-polar spindle formation, chromatin bridges, and lagging chromosomes

• following adeno-cre infection of isolated E14.5 cardiomyocytes, cells with micronuclei in telophase

cellular

abnormal cell nucleus morphology ( J:369747 )

• following adeno-cre infection of isolated E14.5 cardiomyocytes, proliferating cardiomyocytes show a higher rate of nuclear defects compared to non-proliferating cells

• following adeno-cre infection of isolated E14.5 cardiomyocytes, spindle and chromosome distribution defects are seen with an increase in the percentage of cells with unaligned chromosomes, multi-polar spindle formation, chromatin bridges, and lagging chromosomes

abnormal mitosis ( J:369747 )

• following adeno-cre infection of isolated E14.5 cardiomyocytes, spindle and chromosome distribution defects are seen with an increase in the percentage of cells with unaligned chromosomes, multi-polar spindle formation, chromatin bridges, and lagging chromosomes

• following adeno-cre infection of isolated E14.5 cardiomyocytes, cells with micronuclei in telophase

abnormal mitotic spindle morphology ( J:369747 )

• following adeno-cre infection of isolated E14.5 cardiomyocytes, an increase in the percentage of cells with multi-polar spindle formations are seen

Genotype
MGI:8226672

cn5

• Allelic Composition: Rbpms^tm1.1Thbr/Rbpms^tm1.1Thbr; Rbpms2^tm2.1Thbr/Rbpms2^tm2.1Thbr; Tg(Myh6-cre)2182Mds/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * FVB/N

cardiovascular system

abnormal myocardial trabeculae morphology ( J:369747 )

• slight disorganization of the layer

thin ventricle myocardium compact layer ( J:369747 )

• reduced thickness in the ventricles and septum at E16.5 and E18.5

• however, the thickness of the trabecular layer is not altered

abnormal fetal cardiomyocyte morphology ( J:369747 )

• disruption of sarcomere integrity in cardiomyocytes at E16.5

• nuclear abnormalities

abnormal fetal cardiomyocyte physiology ( J:369747 )

• expression analysis indicates defects in cardiomyocyte-specific alternative splicing

muscle

abnormal myocardial trabeculae morphology ( J:369747 )

• slight disorganization of the layer

thin ventricle myocardium compact layer ( J:369747 )

• reduced thickness in the ventricles and septum at E16.5 and E18.5

• however, the thickness of the trabecular layer is not altered

abnormal sarcomere morphology ( J:369747 )

• disruption of sarcomere integrity in cardiomyocytes at E16.5

Genotype
MGI:8226669

cn6

• Allelic Composition: Rbpms^tm1.1Thbr/Rbpms^tm1.1Thbr; Rbpms2^tm2.1Thbr/Rbpms2^tm2.1Thbr; Tg(myl7.L-cre)1118Tmhn/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * MF1

cardiovascular system

abnormal myocardial trabeculae morphology ( J:369747 )

• slight disorganization of the layer

thin ventricle myocardium compact layer ( J:369747 )

• reduced thickness in the ventricles and septum at E14.5

• however, the thickness of the trabecular layer is not altered

muscle

abnormal myocardial trabeculae morphology ( J:369747 )

• slight disorganization of the layer

thin ventricle myocardium compact layer ( J:369747 )

• reduced thickness in the ventricles and septum at E14.5

• however, the thickness of the trabecular layer is not altered

Genotype
MGI:8226627

cn7

• Allelic Composition: Rbpms^tm1.1Thbr/Rbpms^tm1.1Thbr; Tg(myl7.L-cre)1118Tmhn/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * MF1

mortality/aging

mortality/aging ( J:369747 )

N • unlike germline null mice, premature death is not seen

cardiovascular system

abnormal myocardial fiber morphology ( J:369747 )

• greater than 2 fold increase in multi-nucleated cardiomyocyes in the adult heart

premature cardiomyocyte maturation ( J:369747 )

• premature increase in the number of binucleated cardiomyocytes at P0

muscle

abnormal myocardial fiber morphology ( J:369747 )

• greater than 2 fold increase in multi-nucleated cardiomyocyes in the adult heart