Rbpms2^tm2.1Thbr
Targeted Allele Detail

Summary

• Symbol: Rbpms2^tm2.1Thbr
• Name: RNA binding protein with multiple splicing 2; targeted mutation 2.1, Thomas Braun
• MGI ID: MGI:8226421
• Synonyms: Rbpms2^flox
• Gene: Rbpms2 Location: Chr9:65536930-65567810 bp, + strand Genetic Position: Chr9, 35.43 cM
• Alliance: Rbpms2^tm2.1Thbr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:369747
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 65557510 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 65558584. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:369747)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rbpms2 Mutation: 202 strains or lines available

References

• Original: J:369747 Lin S, et al., Rbpms2 prevents major cardiac defects in cardiomyocyte-specific Rbpms-deficient mice. Dev Cell. 2025 Jun 24
• All: 1 reference(s)