Phenotypes associated with this allele

• Allele Symbol: Gm15915^em1Bcgen
• Allele Name: endonuclease-mediated mutation 1, Biocytogen LLC
• Allele ID: MGI:8215376
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gm15915^em1Bcgen/Gm15915^em1Bcgen Tg(Mx1-cre)1Cgn/0	involves: C57BL/6J * CBA/J	MGI:8215402

Genotype
MGI:8215402

cn1

• Allelic Composition: Gm15915^em1Bcgen/Gm15915^em1Bcgen; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: C57BL/6J * CBA/J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

decreased hematopoietic stem cell proliferation ( J:340249 )

• decrease in the colony forming ability of bone marrow cells when culture on complete methylcellulose-based medium, with a decrease in the number of BFU-E, CFU-G, and CFU-GEMM colonies

abnormal erythropoiesis ( J:340249 )

• bone marrow or megakaryocyte-erythroid progenitors cultures on methylcellulose-based medium with erythropoietin produce fewer and smaller colonies

abnormal stress erythropoiesis ( J:340249 )

• decrease in erythrocyte numbers is more severe following phenylhydrazine treatment and noncompetitive transplantation

abnormal bone marrow cell morphology/development ( J:340249 )

decreased common myeloid progenitor cell number ( J:340249 )

• in the bone marrow

decreased bone marrow cell number ( J:340249 )

• decrease in the percentages of most hematopoietic stem and progenitor cells, including LT-HSCs, ST-HSCs, MPP2, MPP3/4, CMPs, MEPs, and MKPs, in the bone marrow

• decrease in the percentages of preMegE, PreCFU-E, and CFU-E/proerythroblast in the bone marrow

• minimal affect on CLPs and GMPs

decreased erythrocyte cell number ( J:340249 )

• decrease in the percentage of terminally differentiated erythrocytes in the bone marrow

• decrease in number of red brood cells and Retic-Es

• however, no effects on white blood cell counts

decreased hemoglobin content ( J:340249 )

decreased hematopoietic stem cell number ( J:340249 )

• decrease in percentages of long term (LT) and short term (ST) hematopoietic stem cells (HSCs) in the bone marrow

• mild effect on HSCs in the fetal liver and spleen

decreased megakaryocyte-erythroid progenitor cell number ( J:340249 )

• in the bone marrow

abnormal bone marrow cell physiology ( J:340249 )

• in a competitive transplantation assay bone marrow cells have a lower reconstitution capacity compared to cells from wild-type controls

cellular

decreased hematopoietic stem cell proliferation ( J:340249 )

• decrease in the colony forming ability of bone marrow cells when culture on complete methylcellulose-based medium, with a decrease in the number of BFU-E, CFU-G, and CFU-GEMM colonies