Phenotypes associated with this allele

• Allele Symbol: Zbtb11^tm1.1Lieg
• Allele Name: targeted mutation 1.1, Graham J Lieschke
• Allele ID: MGI:8213606
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Commd10^Tg(Vav1-icre)A2Kio/Commd10^+ Zbtb11^tm1.1Lieg/Zbtb11^tm1.1Lieg	involves: C57BL/6J * C57BL/10 * CBA/Ca	MGI:8213612

Genotype
MGI:8213612

cn1

• Allelic Composition: Commd10^{Tg(Vav1-icre)A2Kio}/Commd10⁺; Zbtb11^tm1.1Lieg/Zbtb11^tm1.1Lieg
• Genetic Background: involves: C57BL/6J * C57BL/10 * CBA/Ca

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

prenatal lethality, complete penetrance ( J:343208 )

• acute lethality by E18.5

• no gross defects detected from E14.5-E16.5

hematopoietic system

decreased hematopoietic stem cell proliferation ( J:343208 )

• HSCs from E14.5 mice are less than doubled in number after 7 days in culture compared to over 1000-fold increase for wild-type cells

• HSCs from E17.5 mice in culture for 7 days fail to produce any viable cells

abnormal definitive hematopoiesis ( J:343208 )

• fetal liver cellularity is significantly decreased from E14.5 to E17.5

• at E16.5 and E17.5 there are significantly fewer fetal liver CD45+ leukocytes

• by E17.5, both lymphoid and myeloid lineages are dramatically depleted

• decrease in the number of lymphoid-primed multipotent progenitors and myeloerythroid progenitors at E16.5 and E17.5 in the fetal liver

• unlike in controls, no increase in hematopoietic progenitor cells between E16.5 and E17.5

• near complete failure of establishment of bone marrow hematopoiesis

decreased common myeloid progenitor cell number ( J:343208 )

• at E16.5 and E17.5 in the fetal liver

abnormal lymphopoiesis ( J:343208 )

• by E17.5, both lymphoid and myeloid lineages are dramatically depleted

abnormal myelopoiesis ( J:343208 )

• by E17.5, both lymphoid and myeloid lineages are dramatically depleted

anemia ( J:343208 )

• severe anemia from E14.5 onwards with the embryos that are notably pale

impaired hematopoiesis ( J:343208 )

• near-complete failure to establish hematopoiesis in the bone marrow

decreased granulocyte monocyte progenitor cell number ( J:343208 )

• at E16.5 and E17.5 in the fetal liver

decreased common lymphocyte progenitor cell number ( J:343208 )

• at E16.5 and E17.5 in the fetal liver

absent hematopoietic stem cells ( J:343208 )

• absence of LSK cells in the bone marrow at E16.5-E17.5 accompanied by few, if any, bone marrow located committed progenitors and significantly fewer mature lineage progenitors

increased hematopoietic stem cell number ( J:343208 )

• more than double the number of LSK multipotent progenitors at E14.5, however, this difference levels off between E16.5 and E17.5

• there are also more HSCs at E14.5 and this difference is sustained through E17.5

abnormal fetal liver hematopoietic progenitor cell morphology ( J:343208 )

• RNAseq analysis indicates HSCs lack the erythroid primed subpopulation

cellular

abnormal mitosis ( J:343208 )

• at E17.5 a greater proportion of fetal liver LSK multipotent progenitors accumulate in G1

• significant increase in the proportion of granulocyte lineage cells in G0 and decrease in the proportion in S/M phase

decreased cell migration ( J:343208 )

• in a transplantation assay HSCs fail to home to either the bone marrow, spleen, or liver

decreased hematopoietic stem cell proliferation ( J:343208 )

• HSCs from E14.5 mice are less than doubled in number after 7 days in culture compared to over 1000-fold increase for wild-type cells

• HSCs from E17.5 mice in culture for 7 days fail to produce any viable cells

liver/biliary system

abnormal liver morphology ( J:343208 )

• fetal liver cellularity is significantly decreased from E14.5 to E17.5

• at E16.5 and E17.5 there are significantly fewer fetal liver CD45+ leukocytes

growth/size/body

fetal growth retardation ( J:343208 )

• significant growth retardation at E17.5 and E18.5 but not from E14.5 to E16.5

immune system

abnormal lymphopoiesis ( J:343208 )

• by E17.5, both lymphoid and myeloid lineages are dramatically depleted

abnormal myelopoiesis ( J:343208 )

• by E17.5, both lymphoid and myeloid lineages are dramatically depleted