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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Cmtr2em2Cya
endonuclease-mediated mutation 2, Cyagen Biosciences
MGI:7583074
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Cmtr2em2Cya/Cmtr2em2Cya
Plekha5Tg(AMH-cre)1Flor/Plekha5+ 		involves: C57BL/6 * C57BL/6J * SJL MGI:8398172
cn2
 		Cmtr2em2Cya/Cmtr2em2Cya
Tg(Ddx4-cre)1Dcas/0 		involves: C57BL/6J * FVB MGI:8398173
cn3
 		Cmtr2em2Cya/Cmtr2em2Cya
Tg(Stra8-icre)1Reb/0 		Not Specified MGI:8398176


Genotype
MGI:8398172
cn1
Allelic
Composition		 Cmtr2em2Cya/Cmtr2em2Cya
Plekha5Tg(AMH-cre)1Flor/Plekha5+
Genetic
Background		 involves: C57BL/6 * C57BL/6J * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cmtr2em2Cya mutation (1 available); any Cmtr2 mutation (33 available)
Plekha5Tg(AMH-cre)1Flor mutation (1 available); any Plekha5 mutation (109 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
reproductive system phenotype ( J:389086 )
N
• 8-week-old males exhibit normal fertility, spermatogenesis, sperm counts, sperm motility, and testicular morphology




Genotype
MGI:8398173
cn2
Allelic
Composition		 Cmtr2em2Cya/Cmtr2em2Cya
Tg(Ddx4-cre)1Dcas/0
Genetic
Background		 involves: C57BL/6J * FVB
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cmtr2em2Cya mutation (1 available); any Cmtr2 mutation (33 available)
Tg(Ddx4-cre)1Dcas mutation (2 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
abnormal spermatocyte morphology ( J:389086 )
• reduction in the number of primary spermatocytes
• increase in apoptosis of spermatocytes
decreased male germ cell number ( J:389086 )
• number of TRA98+ germ cells is reduced
• germ cells begin to decline starting from P12, when zygotene spermatocytes predominate in the seminiferous tubules; no differences in the numbers of undifferentiated spermatogonia, differentiating spermatogonia, or total spermatogonia but a reduction in numbers of spermatocytes
azoospermia ( J:389086 )
• complete absence of sperm in the epididymis
• non-obstructive azoospermia
decreased spermatid number ( J:389086 )
• complete absence of both round and elongated spermatids
abnormal male meiosis ( J:389086 )
• pachytene-like spermatocytes show defective crossover formation
• spermatocytes exhibit incomplete meiotic recombination
increased testis apoptosis ( J:389086 )
• increase in apoptosis levels in testes
abnormal seminiferous tubule morphology ( J:389086 )
• severe seminiferous tubule disorganization with extensive vacuolization
small testis ( J:389086 )
• reduction in testis size and testis-to-body weight ratio in 8-week-old mice
arrest of male meiosis ( J:389086 )
• although meiosis is initiated and spermatocytes are seen, no postmeiotic germ cells are detected, indicating meiotic arrest
• arrest of the first wave of spermatogenesis at the zygotene spermatocyte stage of prophase I of meiosis
male infertility ( J:389086 )
• 8-week-old males are completely sterile

endocrine/exocrine glands
increased testis apoptosis ( J:389086 )
• increase in apoptosis levels in testes
abnormal seminiferous tubule morphology ( J:389086 )
• severe seminiferous tubule disorganization with extensive vacuolization
small testis ( J:389086 )
• reduction in testis size and testis-to-body weight ratio in 8-week-old mice

homeostasis/metabolism
impaired DNA repair ( J:389086 )
• autosomal DNA damage repair is impaired during pachytene and causes meiotic arrest at the zygotene stage

cellular
abnormal spermatocyte morphology ( J:389086 )
• reduction in the number of primary spermatocytes
• increase in apoptosis of spermatocytes
decreased male germ cell number ( J:389086 )
• number of TRA98+ germ cells is reduced
• germ cells begin to decline starting from P12, when zygotene spermatocytes predominate in the seminiferous tubules; no differences in the numbers of undifferentiated spermatogonia, differentiating spermatogonia, or total spermatogonia but a reduction in numbers of spermatocytes
azoospermia ( J:389086 )
• complete absence of sperm in the epididymis
• non-obstructive azoospermia
decreased spermatid number ( J:389086 )
• complete absence of both round and elongated spermatids
abnormal male meiosis ( J:389086 )
• pachytene-like spermatocytes show defective crossover formation
• spermatocytes exhibit incomplete meiotic recombination
arrest of male meiosis ( J:389086 )
• although meiosis is initiated and spermatocytes are seen, no postmeiotic germ cells are detected, indicating meiotic arrest
• arrest of the first wave of spermatogenesis at the zygotene spermatocyte stage of prophase I of meiosis
increased testis apoptosis ( J:389086 )
• increase in apoptosis levels in testes
impaired DNA repair ( J:389086 )
• autosomal DNA damage repair is impaired during pachytene and causes meiotic arrest at the zygotene stage




Genotype
MGI:8398176
cn3
Allelic
Composition		 Cmtr2em2Cya/Cmtr2em2Cya
Tg(Stra8-icre)1Reb/0
Genetic
Background		 Not Specified
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cmtr2em2Cya mutation (1 available); any Cmtr2 mutation (33 available)
Tg(Stra8-icre)1Reb mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
abnormal spermatocyte morphology ( J:389086 )
• spermatocyte numbers are decreased
decreased male germ cell number ( J:389086 )
• number of total germ cells (TRA98+) is reduced in testes
• germ cells begin to decline starting from P12, when zygotene spermatocytes predominate in the seminiferous tubules
• however, numbers of undifferentiated spermatogonia, differentiating spermatogonia, and total spermatogonia are comparable to controls
decreased spermatid number ( J:389086 )
• seminiferous tubules show complete absence of both round and elongated spermatids
arrest of male meiosis ( J:389086 )
• although meiosis is initiated and spermatocytes are seen, no postmeiotic germ cells are detected, indicating meiotic arrest
abnormal seminiferous tubule morphology ( J:389086 )
• seminiferous tubules are severely disorganized, accompanied by extensive vacuolization

endocrine/exocrine glands
abnormal seminiferous tubule morphology ( J:389086 )
• seminiferous tubules are severely disorganized, accompanied by extensive vacuolization

cellular
abnormal spermatocyte morphology ( J:389086 )
• spermatocyte numbers are decreased
decreased male germ cell number ( J:389086 )
• number of total germ cells (TRA98+) is reduced in testes
• germ cells begin to decline starting from P12, when zygotene spermatocytes predominate in the seminiferous tubules
• however, numbers of undifferentiated spermatogonia, differentiating spermatogonia, and total spermatogonia are comparable to controls
decreased spermatid number ( J:389086 )
• seminiferous tubules show complete absence of both round and elongated spermatids
arrest of male meiosis ( J:389086 )
• although meiosis is initiated and spermatocytes are seen, no postmeiotic germ cells are detected, indicating meiotic arrest




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Send questions and comments to User Support. 		last database update
07/08/2026
MGI 6.24 		The Jackson Laboratory