Analysis Tools
reproductive system
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• approximately 4 orders of magnitude less sperm are recovered from the epididymis
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• adult testes show an almost complete absence of haploid spermatids
• a few later-stage pachytene cells and round spermatids are occasionally detected
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• although P12 testes still contain cells with general nuclear distributions of gammaH2AX typical of leptotene and zygotene, ~90% of those cells that contain a sex body exhibit a less mature elongated "tadpole-shaped" sex body structure
• P12 whole testes show an ~20% increase in Tra2b gene expression and reduced expression of key meiotic genes
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• PAS staining indicates apoptotic pachytene spermatocytes in stage IV arrested tubules
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• at P12, some zygotene cells already show evidence of cell detachment into the lumen of seminiferous tubules
• by P14, large numbers of germ cells have sloughed off into the lumen, suggesting that germ cell development is disrupted at the zygotene/pachytene boundary, followed by cell death and sloughing off during pachytene
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small testis
(
J:360846
)
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• adult testes are significantly smaller than wild type testes
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• adult males (9-25 weeks of age) show significantly lower testis:body weight ratios than wild-type males
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• adult testis sections show a major block at stage IV of spermatogenesis; however, rare tubules containing late surviving pachytene cells and some round spermatids are observed, indicating low level leakage of stage IV arrest
• besides becoming apoptotic, pachytene cells expressing high levels of TRA2B protein are found to detach into the lumen of seminiferous tubules
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• in stage IV arrested tubules, the most advanced type of germ cells present are spermatocytes in the pachytene stage of meiotic prophase
• failure to proceed through meiosis is associated with increased Tra2b mRNA expression leading to accumulation of toxin levels of TRA2B protein and aberrant Tra2b-dependent splicing patterns
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• adult females fail to produce litters when mated with wild-type males
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• adult males fail to produce litters when mated with wild-type females
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cellular
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• approximately 4 orders of magnitude less sperm are recovered from the epididymis
|
|
|
• adult testes show an almost complete absence of haploid spermatids
• a few later-stage pachytene cells and round spermatids are occasionally detected
|
|
|
• although P12 testes still contain cells with general nuclear distributions of gammaH2AX typical of leptotene and zygotene, ~90% of those cells that contain a sex body exhibit a less mature elongated "tadpole-shaped" sex body structure
• P12 whole testes show an ~20% increase in Tra2b gene expression and reduced expression of key meiotic genes
|
|
|
• in stage IV arrested tubules, the most advanced type of germ cells present are spermatocytes in the pachytene stage of meiotic prophase
• failure to proceed through meiosis is associated with increased Tra2b mRNA expression leading to accumulation of toxin levels of TRA2B protein and aberrant Tra2b-dependent splicing patterns
|
|
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• PAS staining indicates apoptotic pachytene spermatocytes in stage IV arrested tubules
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endocrine/exocrine glands
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• at P12, some zygotene cells already show evidence of cell detachment into the lumen of seminiferous tubules
• by P14, large numbers of germ cells have sloughed off into the lumen, suggesting that germ cell development is disrupted at the zygotene/pachytene boundary, followed by cell death and sloughing off during pachytene
|
small testis
(
J:360846
)
|
|
• adult testes are significantly smaller than wild type testes
|
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• adult males (9-25 weeks of age) show significantly lower testis:body weight ratios than wild-type males
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