Analysis Tools
homeostasis/metabolism
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• primary brown adipocytes from tamoxifen-treated mice show a significant reduction in norepinephrine (NE)-induced uncoupled respiration relative to control cells
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Allele Symbol Allele Name Allele ID |
Crls1tm1Geno targeted mutation 1, Genoway MGI:6356533 |
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| Summary |
3 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• primary brown adipocytes from tamoxifen-treated mice show a significant reduction in norepinephrine (NE)-induced uncoupled respiration relative to control cells
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• chow-fed mice show a significant reduction in subcutaneous white adipose tissue (scWAT) weight, and this phenotype is exacerbated on a HFD
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• interscapular brown adipose tissue (iBAT) is distinctly paler than that in control mice, due to a virtual ablation of all cardiolipin species
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• brown adipocytes exhibit distorted cellular and lipid droplet morphology in iBAT, as a result of mitochondrial dysfunction
• however, white adipose depots are not severely affected
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• mtDNA content is markedly decreased in iBAT
• however, mtDNA levels in epididymal white adipose tissue (eWAT) are normal
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• chow-fed mice show a significant reduction in epididymal white adipose tissue (eWAT) weight, and this phenotype is exacerbated on a HFD
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• chow-fed mice show a significant reduction in iBAT weight
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• following i.p. injection with the beta3 agonist CL-316,243 to specifically stimulate glucose uptake into thermogenic fat, mice exhibit significantly lower glucose uptake into iBAT than controls
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• in interscapular brown adipose tissue (iBAT), expression profile of genes linked to thermogenesis, adipogenesis, and mitochondrial respiration more closely resemble that of white fat than brown fat
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• mice show a marked reduction in norepinephrine (NE)-induced uncoupled respiration and heat production relative to controls
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| N |
• chow-fed mice show no significant alterations in energy expenditure relative to control mice
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• mice fed a 60% high-fat diet (HFD) gain significantly less weight and have more lean mass but less fat mass than HFD-fed controls
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• mice show a significantly greater drop in core body temperature than controls in response to acute cold exposure (a 22 to 4 degrees Celsius challenge for 1.5 h), indicating reduced cold tolerance
• cold exposure fails to induce Ucp1 transcription in iBAT or subcutaneous white adipose tissue (scWAT), unlike in control mice
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• mice fed a HFD show increased fasting blood glucose levels
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• mice show a marked reduction in norepinephrine (NE)-induced uncoupled respiration relative to controls
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• mice housed in metabolic cages show a marked reduction in the daily respiratory quotient (RQ) biorhythm relative to controls, indicating decreased metabolic flexibility
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• chow-fed mice show decreased insulin sensitivity relative to control mice
• mice fed a 60% high-fat diet (HFD) are completely refractory to insulin
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• mtDNA content is markedly decreased in iBAT
• however, mtDNA levels in epididymal white adipose tissue (eWAT) are normal
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• following i.p. injection with the beta3 agonist CL-316,243 to specifically stimulate glucose uptake into thermogenic fat, mice exhibit significantly lower glucose uptake into iBAT than controls
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• mitochondria isolated from iBAT show disrupted cristae structure
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• mitochondria isolated from iBAT show reduced mitochondrial mass (as measured by citrate synthase activity)
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• mitochondria isolated from iBAT show altered respiratory chain complex and super complex formation and reduced GDP-inhibitable (UCP1-linked) respiration
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| N |
• chow-fed mice show no significant alterations in body weight or composition relative to control mice
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• mice fed a 60% high-fat diet (HFD) gain significantly less weight and have more lean mass but less fat mass than HFD-fed controls
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• chow-fed mice show increased liver weight relative to control mice, and this phenotype is exacerbated on a HFD
• however, liver triglyceride levels are normal
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• chow-fed mice show increased liver weight relative to control mice, and this phenotype is exacerbated on a HFD
• however, liver triglyceride levels are normal
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• chow-fed mice show a significant reduction in subcutaneous white adipose tissue (scWAT) weight, and this phenotype is exacerbated on a HFD
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| N |
• chow-fed mice show no significant alterations in food intake or physical activity relative to control mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• on a chow diet, tamoxifen-treated mice show no alterations in subcutaneous or epididymal white adipose tissue weight relative to control mice
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• adult tamoxifen-treated mice exhibit a distinctly paler interscapular brown adipose tissue (iBAT) than control mice
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• on a chow diet, tamoxifen-treated mice show a significant reduction in iBAT weight relative to control mice
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• chow-fed mice show decreased insulin sensitivity relative to control mice
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• tamoxifen-treated mice exhibit reduced iBAT mitochondrial mass (as measured by citrate synthase activity)
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| N |
• on a chow diet, tamoxifen-treated mice show no alterations in liver weight relative to control mice
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 05/14/2024 MGI 6.23 |
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