Analysis Tools
cellular
 N |
• number of apoptotic testicular cells at age 1 and 2 weeks
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• reduced number of cells expressing spermatid-specific markers Tp2 and Prm2 amongst dissociated cells from purified seminiferous tubules
• reduced fraction of seminiferous tubules containing elongating spermatids
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• 6-fold fewer pachytene spermatocytes compared to wild-type
• significant reduction in pachytene spermatocytes amongst dissociated cells from purified seminiferous tubules
• reduced number of cells expressing spermatocyte-specific markers Spo11 and Sycp3 amongst dissociated cells from purified seminiferous tubules
• reduced number of cells expressing spermatocyte-specific markers Stra8, Spo11 and Sycp3 amongst FACS sorted 4N spermatocytes
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• reduced number of cells expressing germ cell marker Ldhc amongst dissociated cells from purified seminiferous tubules
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• around 6-fold fewer round and elongated spermatids than wild-type
• 6-fold fewer pachytene spermatocytes compared to wild-type
• significant reduction in pachytene spermatocytes amongst dissociated cells from purified seminiferous tubules
• reduced fraction of seminiferous tubules containing elongating spermatids
• reduced number of cells expressing Ldhc (germ cell marker), Spo11 and Sycp3 (spermatocyte-specific markers), and Tp2 and Prm2 (spermatid-specific markers) amongst dissociated cells from purified seminiferous tubules
• normal number of leptotene/zygotene spermatocytes
• normal number of spermatogonia at age 1 and 2 weeks
• normal number of apoptotic testicular cells at age 1 and 2 weeks
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• sperm count reduced 10-fold to around 10% of wild-type
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• reduced expression of NMD (nonsense-mediated decay) target transcripts Atf4, Cdh11, Ern2, Smad5 and Smad7 in FACS sorted spermatocytes from 4-week old mice
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• around 3-fold increase in apoptotic male germ cells per tubule at age 4 weeks
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growth/size/body
| N |
• body weight and overall morphology
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reproductive system
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• reduced number of cells expressing spermatid-specific markers Tp2 and Prm2 amongst dissociated cells from purified seminiferous tubules
• reduced fraction of seminiferous tubules containing elongating spermatids
|
|
|
• 6-fold fewer pachytene spermatocytes compared to wild-type
• significant reduction in pachytene spermatocytes amongst dissociated cells from purified seminiferous tubules
• reduced number of cells expressing spermatocyte-specific markers Spo11 and Sycp3 amongst dissociated cells from purified seminiferous tubules
• reduced number of cells expressing spermatocyte-specific markers Stra8, Spo11 and Sycp3 amongst FACS sorted 4N spermatocytes
|
|
|
• reduced number of cells expressing germ cell marker Ldhc amongst dissociated cells from purified seminiferous tubules
|
|
|
• around 6-fold fewer round and elongated spermatids than wild-type
• 6-fold fewer pachytene spermatocytes compared to wild-type
• significant reduction in pachytene spermatocytes amongst dissociated cells from purified seminiferous tubules
• reduced fraction of seminiferous tubules containing elongating spermatids
• reduced number of cells expressing Ldhc (germ cell marker), Spo11 and Sycp3 (spermatocyte-specific markers), and Tp2 and Prm2 (spermatid-specific markers) amongst dissociated cells from purified seminiferous tubules
• normal number of leptotene/zygotene spermatocytes
• normal number of spermatogonia at age 1 and 2 weeks
• normal number of apoptotic testicular cells at age 1 and 2 weeks
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• sperm count reduced 10-fold to around 10% of wild-type
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|
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• around 3-fold increase in apoptotic male germ cells per tubule at age 4 weeks
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• significant reduction in pachytene spermatocytes amongst dissociated cells from purified seminiferous tubules
• 6-fold fewer pachytene spermatocytes compared to wild-type
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