Analysis Tools
nervous system
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• ability of the serotonin receptor 2C agonist mCPP to depolarized Pomc-expressing neurons is abolished in mutants
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Allele Symbol Allele Name Allele ID |
Htr2ctm2Jke targeted mutation 2, Joel K Elmquist MGI:5569357 |
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| Summary |
6 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• ability of the serotonin receptor 2C agonist mCPP to depolarized Pomc-expressing neurons is abolished in mutants
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• a high rate of seizure-induced mortality is observed from 6 weeks through 17 weeks
• Background Sensitivity: with further backcrossing to enrich the C57BL/6J background content, the mortality phenotype is almost eliminated
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• on an obesogenic (high fat,high sugar) diet, higher rate of obesity is observed compared to controls
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• on an obesogenic (high fat,high sugar) diet, higher rate of obesity is observed compared to controls
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• mice show enhanced hyperglycemia incidence when fed an obesogenic (high fat,high sugar) diet
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• daily food intake is increased in chow-fed mice given tamoxifen at 11 weeks and monitored from 12-20 weeks
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N |
• mice given tamoxifen at 11 weeks and switched to a high fat, high sugar (HFHS) diet 1 week after exhibit higher body weights after 6 weeks on the HFHS diet
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• mice given tamoxifen at 11 weeks and switched to a high fat, high sugar (HFHS) diet 1 week later exhibit higher body weights after 6 weeks on the HFHS diet
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N |
• chow-fed mutants receiving tamoxifen at 11 weeks do not show changes in respiratory exchange ratio or physical activity from 12-20 weeks
• tamoxifen-treated mutants that are switched to a high fat, high sugar (HFHS) diet exhibit hyperphagia but do not show a further increase in VO2 and VCO2
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• two weeks after tamoxifen treatment, chow-fed mutants have elevated levels
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• two weeks after tamoxifen treatment, chow-fed mutants have elevated levels
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• hyperinsulinemia is observed 4 weeks after tamoxifen treatment in chow-fed mutants
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
 N |
• mice given tamoxifen at 11 weeks and switched to a high fat, high sugar (HFHS) diet 1 week later exhibit higher body weights after 6 weeks on the HFHS diet
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• mice given tamoxifen at 11 weeks and switched to a high fat, high sugar (HFHS) diet 1 week after exhibit higher body weights after 6 weeks on the HFHS diet
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N |
• chow-fed mutants receiving tamoxifen at 11 weeks do not show changes in respiratory exchange ratio or physical activity from 12-20 weeks
• tamoxifen-treated mutants that are switched to a high fat, high sugar (HFHS) diet exhibit hyperphagia but do not show a further increase in VO2 and VCO2
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• two weeks after tamoxifen treatment, chow-fed mutants have elevated levels
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• two weeks after tamoxifen treatment, chow-fed mutants have elevated levels
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• hyperinsulinemia is observed 4 weeks after tamoxifen treatment in chow-fed mutants
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• daily food intake is increased in chow-fed mice given tamoxifen at 11 weeks and monitored from 12-20 weeks
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• daily food intake is elevated relative to controls when fed a regular chow diet; when diet is transitioned to a high fat, high sugar (HFHS) diet for 1 week, food intake is significantly increased relative to controls
• 20 week-old mutants which had been fed an HFHS diet for 12 weeks show a higher food intake compared to controls
• administration of d-flenfluaramine or the more specific serotonin 2C receptor agonist mCPP strongly suppresses food intake in control mice, but mutants are not affected
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• total activity due to increased dark cycle activity is increased compared to controls when fed a regular chow diet
• 20 week-old mutants which had been fed an HFHS diet for 12 weeks show increased total physical activity compared to controls; this increase is restricted to the dark phase
• when animals are switched to a high fat high sugar diet for 1 week, physical activity does not increase relative to the chow diet
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N |
• when fed a regular chow diet, body weights of mutants are similar to controls at weaning and between 8 and 20 weeks of age
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• HFHS-fed (high fat, high sugar) animals have significantly increased body weights at 4 weeks after introduction of this diet; at 20 weeks on the HFHS-diet, mutants are 25% heavier than controls due to adiposity
• 20 week-old mutants which had been fed an HFHS diet for 12 weeks have increased body weights compared to controls
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• HFHS-fed (high fat, high sugar) animals have significantly increased body weights at 4 weeks after introduction of this diet
• chow-fed mutants transitioned acutely to HFHS food display similarly increased body weights after 1 week of the new diet
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N |
• on a regular chow diet, mutants and controls have similar respiratory exchange ratios when fed a regular chow diet
• chow-fed or HFHS-fed (high fat, high sugar) animals have similar circulating leptin levels
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• HFHS-fed (high fat, high sugar) animals have significantly increased body weights at 4 weeks after introduction of this diet
• chow-fed mutants transitioned acutely to HFHS food display similarly increased body weights after 1 week of the new diet
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• at the beginning of the light phase (7 am), chow-fed mice show modestly higher glucose levels than controls; levels are significantly elevated in postabsorptive (4-5 hour morning-fasted animals) but not in overnight (12-24 h) fasted mice
• high fat, high sugar (HFHS) fed mutants also have elevated glucose levels in the postabsorptive period compared to HFHS-fed controls
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• higher plasma glucagon levels are detected in postabsorptive chow-fed mutants (4-5 hour morning-fasted); levels are comparable to controls with overnight fasting
• HFHS-fed (high fat, high sugar) animals do not show additionally increased plasma glucagon levels compared to chow-fed mutants
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• higher plasma insulin levels are detected in postabsorptive chow-fed mutants (4-5 hour morning-fasted); levels are comparable to controls with overnight fasting
• HFHS-fed (high fat, high sugar) animals have a greater increase in plasma insulin compared to chow-fed mutants
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• increased energy intake and expenditure are observed when fed a regular chow diet
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• 20 week-old mutants which had been fed an HFHS diet for 12 weeks have lower oxygen consumption relative to body weight compared to controls on the same diet while carbon dioxide production is similar to controls
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• VO2 (and VCO2) are increased compared to controls when fed a regular chow diet
• when animals are switched to a high fat high sugar diet for 1 week, oxygen consumption/ C02 production does not increase relative to the chow diet
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• on the high fat high sugar diet for 1 week, respiratory exchange ratio (RER) is slightly decreased in mutants and controls compared to animals on a regular chow diet, but over a 24 hour period, mutants show a modest increase relative to controls
• 20 week-old mutants which had been fed an HFHS (high fat, high sugar) diet for 12 weeks have a higher RER during the dark phase
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• hyperinsulinemic-euglycemic clamp experiments in postabsorptive and overnight-fasted 8-9 week old mice show reduced insulin sensitivity
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N |
• animals are born at Mendelian frequency and show no increased mortality compared to controls
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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N |
• animals are born at Mendelian frequency and show no increased mortality compared to controls
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N |
• when fed a regular chow diet, body weights of mutants are similar to controls at weaning and between 8 and 20 weeks of age
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• HFHS-fed (high fat, high sugar) animals have significantly increased body weights at 4 weeks after introduction of this diet; at 20 weeks on the HFHS-diet, mutants are 25% heavier than controls due to adiposity
• 20 week-old mutants which had been fed an HFHS diet for 12 weeks have increased body weights compared to controls
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|
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• HFHS-fed (high fat, high sugar) animals have significantly increased body weights at 4 weeks after introduction of this diet
• chow-fed mutants transitioned acutely to HFHS food display similarly increased body weights after 1 week of the new diet
|
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|
• daily food intake is elevated relative to controls when fed a regular chow diet; when diet is transitioned to a high fat, high sugar (HFHS) diet for 1 week, food intake is significantly increased relative to controls
• 20 week-old mutants which had been fed an HFHS diet for 12 weeks show a higher food intake compared to controls
• administration of d-flenfluaramine or the more specific serotonin 2C receptor agonist mCPP strongly suppresses food intake in control mice, but mutants are not affected
|
|
|
• total activity due to increased dark cycle activity is increased compared to controls when fed a regular chow diet
• 20 week-old mutants which had been fed an HFHS diet for 12 weeks show increased total physical activity compared to controls; this increase is restricted to the dark phase
• when animals are switched to a high fat high sugar diet for 1 week, physical activity does not increase relative to the chow diet
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N |
• on a regular chow diet, mutants and controls have similar respiratory exchange ratios when fed a regular chow diet
• chow-fed or HFHS-fed (high fat, high sugar) animals have similar circulating leptin levels
|
|
|
• HFHS-fed (high fat, high sugar) animals have significantly increased body weights at 4 weeks after introduction of this diet
• chow-fed mutants transitioned acutely to HFHS food display similarly increased body weights after 1 week of the new diet
|
|
|
• at the beginning of the light phase (7 am), chow-fed mice show modestly higher glucose levels than controls; levels are significantly elevated in postabsorptive (4-5 hour morning-fasted animals) but not in overnight (12-24 h) fasted mice
• high fat, high sugar (HFHS) fed mutants also have elevated glucose levels in the postabsorptive period compared to HFHS-fed controls
|
|
|
• higher plasma glucagon levels are detected in postabsorptive chow-fed mutants (4-5 hour morning-fasted); levels are comparable to controls with overnight fasting
• HFHS-fed (high fat, high sugar) animals do not show additionally increased plasma glucagon levels compared to chow-fed mutants
|
|
|
• higher plasma insulin levels are detected in postabsorptive chow-fed mutants (4-5 hour morning-fasted); levels are comparable to controls with overnight fasting
• HFHS-fed (high fat, high sugar) animals have a greater increase in plasma insulin compared to chow-fed mutants
|
|
|
• increased energy intake and expenditure are observed when fed a regular chow diet
|
|
|
• 20 week-old mutants which had been fed an HFHS diet for 12 weeks have lower oxygen consumption relative to body weight compared to controls on the same diet while carbon dioxide production is similar to controls
|
|
|
• VO2 (and VCO2) are increased compared to controls when fed a regular chow diet
• when animals are switched to a high fat high sugar diet for 1 week, oxygen consumption/ C02 production does not increase relative to the chow diet
|
|
|
• on the high fat high sugar diet for 1 week, respiratory exchange ratio (RER) is slightly decreased in mutants and controls compared to animals on a regular chow diet, but over a 24 hour period, mutants show a modest increase relative to controls
• 20 week-old mutants which had been fed an HFHS (high fat, high sugar) diet for 12 weeks have a higher RER during the dark phase
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• hyperinsulinemic-euglycemic clamp experiments in postabsorptive and overnight-fasted 8-9 week old mice show reduced insulin sensitivity
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 05/14/2024 MGI 6.23 |
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