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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Sppl2atm1Basc
targeted mutation 1, Bernd Schroder
MGI:5491887
Summary 3 genotypes


Genotype
MGI:5491937
hm1
Allelic
Composition		 Sppl2atm1Basc/Sppl2atm1Basc
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * C57BL/6NCrl
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sppl2atm1Basc mutation (0 available); any Sppl2a mutation (46 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype

Atrophic Peyer's patches in Sppl2atm1Basc/Sppl2atm1Basc mice

homeostasis/metabolism
abnormal B cell calcium ion homeostasis ( J:194664 )
• calcium ion entry across the plasma membrane of T1 B cells is decreased compared to in wild-type mice

immune system
arrested B cell differentiation ( J:194664 )
• maturation block at T1 stage
• transplant experiments suggest cell-intrinsic defect
decreased B cell number ( J:194664 )
• in the blood, bone marrow, spleen, lymph nodes and peritoneum
decreased mature B cell number ( J:194664 )
• in the spleen and bone marrow
decreased B-1 B cell number ( J:194664 )
• in the peritoneum
decreased B-2 B cell number ( J:194664 )
• in the peritoneum
decreased T cell number ( J:194664 )
• in the spleen and lymph nodes
abnormal B cell physiology ( J:194664 )
• B cells accumulate endosomal vacuoles containing Cd74 N terminal fragment compared with wild-type cells
abnormal B cell calcium ion homeostasis ( J:194664 )
• calcium ion entry across the plasma membrane of T1 B cells is decreased compared to in wild-type mice
decreased IgA level ( J:194664 )
• in untreated mice
decreased IgG1 level ( J:194664 )
• in untreated mice
• in mice exposed to TNP-Ficoll or TNP-KHL (after 14 and 28 days)
decreased IgG2a level ( J:194664 )
• in untreated mice
• in mice exposed to TNP-Ficoll or TNP-KHL (after 14 and 28 days)
decreased IgG2b level ( J:194664 )
• in untreated mice
• in mice exposed to TNP-Ficoll or TNP-KHL (after 14 and 28 days)
decreased IgG3 level ( J:194664 )
• in untreated mice
• in mice exposed to TNP-Ficoll or TNP-KHL (after 14 and 28 days)
decreased IgM level ( J:194664 )
• in untreated mice
• in mice exposed to TNP-Ficoll or TNP-KHL (after 14, but not 28, days)

hematopoietic system
arrested B cell differentiation ( J:194664 )
• maturation block at T1 stage
• transplant experiments suggest cell-intrinsic defect
decreased B cell number ( J:194664 )
• in the blood, bone marrow, spleen, lymph nodes and peritoneum
decreased mature B cell number ( J:194664 )
• in the spleen and bone marrow
decreased B-1 B cell number ( J:194664 )
• in the peritoneum
decreased B-2 B cell number ( J:194664 )
• in the peritoneum
decreased T cell number ( J:194664 )
• in the spleen and lymph nodes
abnormal B cell physiology ( J:194664 )
• B cells accumulate endosomal vacuoles containing Cd74 N terminal fragment compared with wild-type cells
abnormal B cell calcium ion homeostasis ( J:194664 )
• calcium ion entry across the plasma membrane of T1 B cells is decreased compared to in wild-type mice
decreased IgA level ( J:194664 )
• in untreated mice
decreased IgG1 level ( J:194664 )
• in untreated mice
• in mice exposed to TNP-Ficoll or TNP-KHL (after 14 and 28 days)
decreased IgG2a level ( J:194664 )
• in untreated mice
• in mice exposed to TNP-Ficoll or TNP-KHL (after 14 and 28 days)
decreased IgG2b level ( J:194664 )
• in untreated mice
• in mice exposed to TNP-Ficoll or TNP-KHL (after 14 and 28 days)
decreased IgG3 level ( J:194664 )
• in untreated mice
• in mice exposed to TNP-Ficoll or TNP-KHL (after 14 and 28 days)
decreased IgM level ( J:194664 )
• in untreated mice
• in mice exposed to TNP-Ficoll or TNP-KHL (after 14, but not 28, days)

digestive/alimentary system




Genotype
MGI:5491938
cx2
Allelic
Composition		 Cd74tm1Liz/Cd74tm1Liz
Sppl2atm1Basc/Sppl2atm1Basc
Genetic
Background		 B6.129-Sppl2atm1Basc Cd74tm1Liz
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd74tm1Liz mutation (5 available); any Cd74 mutation (37 available)
Sppl2atm1Basc mutation (0 available); any Sppl2a mutation (46 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype

Sppl2atm1Basc/Sppl2atm1Basc Cd74tm1Liz/Cd74tm1Liz B cells show normal morphology compared to the increased vacuoles in Sppl2atm1Basc/Sppl2atm1Basc B cells

immune system
immune system phenotype ( J:194664 )
N
• B cells exhibit the same number of vacuoles per cells as wild-type mice and Cd74tm1Liz homozygotes
• calcium ion flux in T1 B cells is restored to normal
• B cell maturation and function are restored
• the size of Peyer's patches is restored
decreased Peyer's patch number ( J:194664 )
• compared with wild-type mice but improved from Sppl2atm1Basc homozygotes
decreased B cell number ( J:194664 )
• compared with wild-type mice but improved from Sppl2atm1Basc homozygotes in the spleen and lymph nodes

hematopoietic system
decreased B cell number ( J:194664 )
• compared with wild-type mice but improved from Sppl2atm1Basc homozygotes in the spleen and lymph nodes

digestive/alimentary system
decreased Peyer's patch number ( J:194664 )
• compared with wild-type mice but improved from Sppl2atm1Basc homozygotes




Genotype
MGI:5698091
cx3
Allelic
Composition		 Sppl2atm1Basc/Sppl2atm1Basc
Sppl2bGt(Ayu21-T160)Imeg/Sppl2bGt(Ayu21-T160)Imeg
Genetic
Background		 B6N.Cg-Sppl2atm1Basc Sppl2bGt(Ayu21-T160)Imeg
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Sppl2atm1Basc mutation (0 available); any Sppl2a mutation (46 available)
Sppl2bGt(Ayu21-T160)Imeg mutation (0 available); any Sppl2b mutation (28 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
decreased B cell number ( J:213519 )
• significant reduction of total B cells in the spleen, lymph nodes, and peritoneal cavity
• B cell numbers similar to mice homozygous only for Sppl2atm1Basc
abnormal mature B cell morphology ( J:213519 )
• numerous endosomal vesicles as is seen in mice homozygous only for Sppl2atm1Basc
decreased immunoglobulin level ( J:213519 )
• levels decreased to near limit of detectability as seen in mice homozygous only for Sppl2atm1Basc

immune system
decreased B cell number ( J:213519 )
• significant reduction of total B cells in the spleen, lymph nodes, and peritoneal cavity
• B cell numbers similar to mice homozygous only for Sppl2atm1Basc
abnormal mature B cell morphology ( J:213519 )
• numerous endosomal vesicles as is seen in mice homozygous only for Sppl2atm1Basc
decreased immunoglobulin level ( J:213519 )
• levels decreased to near limit of detectability as seen in mice homozygous only for Sppl2atm1Basc




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Send questions and comments to User Support. 		last database update
09/30/2025
MGI 6.24 		The Jackson Laboratory