Phenotypes associated with this allele

• Allele Symbol: Vps18^tm1.1Wtao
• Allele Name: targeted mutation 1.1, Wufan Tao
• Allele ID: MGI:5431976
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Vps18^tm1.1Wtao/Vps18^tm1.1Wtao Tg(Pcp2-cre)2Mpin/0	B.129-Vps18^tm1.1Wtao Tg(Pcp2-cre)2Mpin	MGI:5431980
cn2	Vps18^tm1.1Wtao/Vps18^tm1.1Wtao Tg(Nes-cre)1Kln/0	involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6 * SJL	MGI:5431979

Genotype
MGI:5431980

cn1

• Allelic Composition: Vps18^tm1.1Wtao/Vps18^tm1.1Wtao; Tg(Pcp2-cre)2Mpin/0
• Genetic Background: B.129-Vps18^tm1.1Wtao Tg(Pcp2-cre)2Mpin

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Neurodegeneration in Vps18^tm1.1Wtao/Vps18^tm1.1Wtao Tg(Nes-cre)1Kln/0 and Vps18^tm1.1Wtao/Vps18^tm1.1Wtao Tg(Pcp2-cre)2Mpin/0 brains

nervous system

nervous system phenotype ( J:186307 )

N • Purkinje cells proliferation is normal

decreased Purkinje cell number ( J:186307 )

• dramatic loss at 1 month

• very few Purkinje cells are present at 3 months

Genotype
MGI:5431979

cn2

• Allelic Composition: Vps18^tm1.1Wtao/Vps18^tm1.1Wtao; Tg(Nes-cre)1Kln/0
• Genetic Background: involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6 * SJL

Small size of Vps18^tm1.1Wtao/Vps18^tm1.1Wtao Tg(Nes-cre)1Kln/0 mice at P10

mortality/aging

postnatal lethality, complete penetrance ( J:186307 )

• despite being born at Mendelian ratios, all mice die before P12

nervous system

decreased brain size ( J:186307 )

• especially in the cerebral cortex and cerebellum region

abnormal hippocampus morphology ( J:186307 )

• at P10, the hippocampus losses its morphological structure

abnormal hippocampus CA3 region morphology ( J:186307 )

• at P1, the CA3 region is split into two layers with more loosely associated neurons compared with wild-type mice

abnormal cerebellum morphology ( J:186307 )

• reduced foliation at P1 and P10

small cerebellum ( J:186307 )

• at P1 and P10

neurodegeneration ( J:186307 )

• severe at P10

abnormal neuron physiology ( J:186307 )

• neurons exhibit reduced apoptosis and endocytosis compared with control cells

increased neuron apoptosis ( J:186307 )

abnormal neuronal migration ( J:186307 )

• impaired

growth/size/body

decreased body size ( J:186307 )

• at P10

postnatal growth retardation ( J:186307 )

cellular

abnormal autophagy ( J:186307 )

• dramatically reduced in neurons

increased neuron apoptosis ( J:186307 )

abnormal neuronal migration ( J:186307 )

• impaired

homeostasis/metabolism

abnormal autophagy ( J:186307 )

• dramatically reduced in neurons