Phenotypes associated with this allele

• Allele Symbol: Rag2^tm1.1Mss
• Allele Name: targeted mutation 1.1, Mark S Schlissel
• Allele ID: MGI:5002798
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rag2^tm1.1Mss/Rag2^tm1.1Mss	involves: 129S6/SvEvTac	MGI:5002823
cx2	Rag2^tm1.1Mss/Rag2^tm1.1Mss Trp53^tm1Tyj/Trp53^tm1Tyj	involves: 129S2/SvPas * 129S6/SvEvTac	MGI:5002824

Genotype
MGI:5002823

hm1

• Allelic Composition: Rag2^tm1.1Mss/Rag2^tm1.1Mss
• Genetic Background: involves: 129S6/SvEvTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal lymphopoiesis ( J:171970 )

increased DN3 thymocyte number ( J:171970 )

• attenuation of transition from double negative to double positive T-cells

• partial blockage between DN3 and DN4

• TCRbeta assembly issue

• problem with V^beta to DJ^beta joining

• also problems in VD^delta to J^delta joining

abnormal pre-B cell morphology ( J:171970 )

• B cell lymphopoiesis impaired at an early stage

decreased pre-B cell number ( J:171970 )

• pro B cells are normal

• pre B cells are reduced 4 fold in number

• blockade occurs when IgH assembly should be taking place

• impaired V^H to DJ^H joining leads to a 10 fold reduction in completion of heavy chain assembly

neoplasm

neoplasm ( J:170462 )

N • homozygotes show no sign of tumorigenesis up to 1 year of age

hematopoietic system

abnormal lymphopoiesis ( J:171970 )

increased DN3 thymocyte number ( J:171970 )

• attenuation of transition from double negative to double positive T-cells

• partial blockage between DN3 and DN4

• TCRbeta assembly issue

• problem with V^beta to DJ^beta joining

• also problems in VD^delta to J^delta joining

abnormal pre-B cell morphology ( J:171970 )

• B cell lymphopoiesis impaired at an early stage

decreased pre-B cell number ( J:171970 )

• pro B cells are normal

• pre B cells are reduced 4 fold in number

• blockade occurs when IgH assembly should be taking place

• impaired V^H to DJ^H joining leads to a 10 fold reduction in completion of heavy chain assembly

endocrine/exocrine glands

increased DN3 thymocyte number ( J:171970 )

• attenuation of transition from double negative to double positive T-cells

• partial blockage between DN3 and DN4

• TCRbeta assembly issue

• problem with V^beta to DJ^beta joining

• also problems in VD^delta to J^delta joining

Genotype
MGI:5002824

cx2

• Allelic Composition: Rag2^tm1.1Mss/Rag2^tm1.1Mss; Trp53^tm1Tyj/Trp53^tm1Tyj
• Genetic Background: involves: 129S2/SvPas * 129S6/SvEvTac

mortality/aging

premature death ( J:170462 )

• 100% of mice die within 16 weeks with aggressive lymphomas

neoplasm

increased lymphoma incidence ( J:170462 )

• 100% of mice die within 16 weeks with aggressive lymphomas

increased T cell derived lymphoma incidence ( J:170462 )

• tumor cells are highly proliferative

• tumor cells express CD4+ and CD8+ but little surface TCR

• tumors are detected at 4-6 weeks in the thymus but not in other organs

• cytogenetic aberrations in tumor cells

cellular

abnormal chromosome morphology ( J:170462 )

• chromosome breaks and fusions in tumor cells

• recurrent translocations on Chromosomes 14 and 16 where TCR genes are located and on Chromosomes 12, 6, and 16 where Ig genes are located

• translocations involve Chromosomes 12 and 14 in particular

aneuploidy ( J:170462 )

• observed in tumor cells

endocrine/exocrine glands

increased T cell derived lymphoma incidence ( J:170462 )

• tumor cells are highly proliferative

• tumor cells express CD4+ and CD8+ but little surface TCR

• tumors are detected at 4-6 weeks in the thymus but not in other organs

• cytogenetic aberrations in tumor cells