Rag2^tm1.1Mss
Targeted Allele Detail

Summary

• Symbol: Rag2^tm1.1Mss
• Name: recombination activating gene 2; targeted mutation 1.1, Mark S Schlissel
• MGI ID: MGI:5002798
• Synonyms: Rag2^c, Rag2^CORE
• Gene: Rag2 Location: Chr2:101455063-101462874 bp, + strand Genetic Position: Chr2, 53.87 cM
• Alliance: Rag2^tm1.1Mss page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:171970
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Hypomorph, Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A cDNA encoding exons 1-383 and an engineered stop codon at position 384 and a floxed neomycin selection cassette replaced the endogenous coding sequence. The resulting allele lacks the sequence encoding amino acids 384-527 of the intact protein. The selection cassette was subsequently removed by cre excision. (J:171970)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rag2 Mutation: 115 strains or lines available

References

• Original: J:171970 Liang HE, et al., The 'dispensable' portion of RAG2 is necessary for efficient V-to-DJ rearrangement during B and T cell development. Immunity. 2002 Nov;17(5):639-51
• All: 6 reference(s)