Phenotypes associated with this allele

• Allele Symbol: Opn4^{tm1.1(cre)Saha}
• Allele Name: targeted mutation 1.1, Samer Hattar
• Allele ID: MGI:4881482
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Opn4^tm1.1(cre)Saha/Opn4^+ Pou4f2^tm2.1Nat/Pou4f2^+	involves: 129 * C57BL/6	MGI:5285910
cn2	Opn4^tm1.1(cre)Saha/Opn4^+ Pou4f2^tm4(DTA)Whk/Pou4f2^+ Tg(CAG-Bgeo/ALPP)1Lbe/0	involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5285913
cn3	Opn4^tm1.1(cre)Saha/Opn4^tm1Yau Pou4f2^tm4(DTA)Whk/Pou4f2^+	involves: 129S/SvEv * C57BL/6	MGI:5285915
cn4	Opn4^tm1.1(cre)Saha/Opn4^+ Pou4f2^tm4(DTA)Whk/Pou4f2^+	involves: 129S/SvEv * C57BL/6	MGI:5285914

Genotype
MGI:5285910

cn1

• Allelic Composition: Opn4^{tm1.1(cre)Saha}/Opn4⁺; Pou4f2^tm2.1Nat/Pou4f2⁺
• Genetic Background: involves: 129 * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal suprachiasmatic nucleus morphology ( J:174940 )

• medial regions of the suprachiasmatic nucleus are not innervated at all

abnormal innervation ( J:174940 )

• the suprachiasmatic nucleus (SCN) is sparsely innervated by Pou4f2-positive ipRGCs (intrinsically photosensitive retinal ganglion cells)

Genotype
MGI:5285913

cn2

• Allelic Composition: Opn4^{tm1.1(cre)Saha}/Opn4⁺; Pou4f2^tm4(DTA)Whk/Pou4f2⁺; Tg(CAG-Bgeo/ALPP)1Lbe/0
• Genetic Background: involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ * C57BL/6

nervous system

abnormal olivary pretectal nucleus morphology ( J:174940 )

• and olivary pretectal nucleus (OPN) projections are eliminated, compared to controls

abnormal innervation ( J:174940 )

• innervation of the intergeniculate leaflet (IGL) is markedly reduced

decreased retina ganglion cell number ( J:174940 )

• all non-M1 retinal ganglion cells are ablated in the retinas, with only about 200 Pou4f2-negative cells detected (200/2000); these remaining fibers innervated the SCN to the same extent as control Pou4f2^tm2.1Nat/+ Opn4^{tm1.1(cre)Saha/+} animals

vision/eye

decreased retina ganglion cell number ( J:174940 )

• all non-M1 retinal ganglion cells are ablated in the retinas, with only about 200 Pou4f2-negative cells detected (200/2000); these remaining fibers innervated the SCN to the same extent as control Pou4f2^tm2.1Nat/+ Opn4^{tm1.1(cre)Saha/+} animals

Genotype
MGI:5285915

cn3

• Allelic Composition: Opn4^{tm1.1(cre)Saha}/Opn4^tm1Yau; Pou4f2^tm4(DTA)Whk/Pou4f2⁺
• Genetic Background: involves: 129S/SvEv * C57BL/6

nervous system

abnormal olivary pretectal nucleus morphology ( J:174940 )

• and olivary pretectal nucleus (OPN) projections are eliminated, compared to controls

• no fibres are observed in the shell of the OPN

abnormal innervation ( J:174940 )

• innervation of the intergeniculate nucleus (IGL) is markedly reduced

decreased retina ganglion cell number ( J:174940 )

• only 200 M1 (Pou4f2-negative) ipRGCs (intrinsically photosensitive retinal ganglion cells) are detected in the retinas; these remaining fibers innervated the SCN to the same extent as control Pou4f2^tm2.1Nat/+ ;Opn4^{tm1.1(cre)Saha/+} animals

vision/eye

decreased retina ganglion cell number ( J:174940 )

• only 200 M1 (Pou4f2-negative) ipRGCs (intrinsically photosensitive retinal ganglion cells) are detected in the retinas; these remaining fibers innervated the SCN to the same extent as control Pou4f2^tm2.1Nat/+ ;Opn4^{tm1.1(cre)Saha/+} animals

Genotype
MGI:5285914

cn4

• Allelic Composition: Opn4^{tm1.1(cre)Saha}/Opn4⁺; Pou4f2^tm4(DTA)Whk/Pou4f2⁺
• Genetic Background: involves: 129S/SvEv * C57BL/6

behavior/neurological

behavior/neurological phenotype ( J:174940 )

N • mutants are able to photoentrain (their circadian phase) like controls; also, activity

impaired pupillary reflex ( J:174940 )

• the papillary light reflex (PLR) is significantly attenuated at high and low light intensities in the middle of the day (zeitgerber time 8, ZT8); in the middle of the night (ZT20), mice show no PLR

abnormal circadian behavior period ( J:174940 )

• animals show a minor deficit in period lengthening under constant light conditions

nervous system

decreased retina ganglion cell number ( J:174940 )

• only 200 M1 (Pou4f2-negative) ipRGCs (intrinsically photosensitive retinal ganglion cells) are detected in the retinas

vision/eye

vision/eye phenotype ( J:174940 )

N • visual acuity is comparable to wild-type animals

impaired pupillary reflex ( J:174940 )

• the papillary light reflex (PLR) is significantly attenuated at high and low light intensities in the middle of the day (zeitgerber time 8, ZT8); in the middle of the night (ZT20), mice show no PLR

decreased retina ganglion cell number ( J:174940 )

• only 200 M1 (Pou4f2-negative) ipRGCs (intrinsically photosensitive retinal ganglion cells) are detected in the retinas