Opn4^{tm1.1(cre)Saha}
Targeted Allele Detail

Summary

• Symbol: Opn4^{tm1.1(cre)Saha}
• Name: opsin 4 (melanopsin); targeted mutation 1.1, Samer Hattar
• MGI ID: MGI:4881482
• Synonyms: Opn4^Cre
• Gene: Opn4 Location: Chr14:34312575-34322099 bp, - strand Genetic Position: Chr14, 20.83 cM, cytoband B
• Alliance: Opn4^{tm1.1(cre)Saha} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:167871
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Opn4^{tm1.1(cre)Saha} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Opn4 (MGI:1353425)

• Mutation details: Cre recombinase preceded by a human beta-globin intron and nuclear localization signal was inserted immediately after the start codon of the Opn4 gene, replacing the open reading frame. An FRT-flanked pgk-neo cassette followed the cre coding sequence. Homologous recombination in ES cells resulted in replacement of the coding region of Opn4 with this construct. After establishing germline transmission of the allele, mice were crossed to Flp-expressing animals to remove the neo cassette with the Flp gene subsequently bred out of the line in creating homozygous mutants. (J:167871)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Opn4 (mouse) Summary of all recombinase alleles driven by Opn4. Comparative matrix view of recombinase activities Alliance mouse Opn4 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Opn4 Mutation: 33 strains or lines available

References

• Original: J:167871 Ecker JL, et al., Melanopsin-expressing retinal ganglion-cell photoreceptors: cellular diversity and role in pattern vision. Neuron. 2010 Jul 15;67(1):49-60
• All: 51 reference(s)