Phenotypes associated with this allele

• Allele Symbol: Tg(Car1-cre)5Flt
• Allele Name: transgene insertion 5, James C Fleet
• Allele ID: MGI:4835007
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Rnf40^tm1.1Sajo/Rnf40^tm1.1Sajo Tg(Car1-cre)5Flt/0	involves: 129S2/SvPas * C57BL/6J	MGI:6781800
cn2	Apc^tm2Rak/Apc^tm2Rak Tg(Car1-cre)5Flt/0	involves: 129/Sv * C57BL/6J * SJL	MGI:4835053
cn3	Apc^tm2Rak/Apc^+ Tg(Car1-cre)5Flt/0	involves: 129/Sv * C57BL/6J * SJL	MGI:4835054

Genotype
MGI:6781800

cn1

• Allelic Composition: Rnf40^tm1.1Sajo/Rnf40^tm1.1Sajo; Tg(Car1-cre)5Flt/0
• Genetic Background: involves: 129S2/SvPas * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

digestive/alimentary system

decreased susceptibility to induced colitis ( J:311838 )

• mice treated with DSS to induce colorectal inflammation and acute colitis show a lower disease activity index compared to control mice, with minimal weight loss and unaffected stool consistency and frequency of occult blood and only mild epithelial damage in the colon

• DSS-treated mice show only low proportions of epithelium with medium to severe signs of inflammation indicating reduced colorectal inflammation

immune system

decreased susceptibility to induced colitis ( J:311838 )

• mice treated with DSS to induce colorectal inflammation and acute colitis show a lower disease activity index compared to control mice, with minimal weight loss and unaffected stool consistency and frequency of occult blood and only mild epithelial damage in the colon

• DSS-treated mice show only low proportions of epithelium with medium to severe signs of inflammation indicating reduced colorectal inflammation

skeleton

increased bone strength ( J:311838 )

• the applied strength when bone deformation is induced (yield load) and before (failure load) and during (Fmax) breaking the bone is higher, indicating that mice have less fragile bones

Genotype
MGI:4835053

cn2

• Allelic Composition: Apc^tm2Rak/Apc^tm2Rak; Tg(Car1-cre)5Flt/0
• Genetic Background: involves: 129/Sv * C57BL/6J * SJL

mortality/aging

premature death ( J:164247 )

• mice are able to survive up to 6 weeks of age

growth/size/body

decreased body weight ( J:164247 )

• body weights are reduced by 12.8% at 3 weeks and by 20% at 6 weeks

neoplasm

increased intestinal adenoma incidence ( J:164247 )

• raised lesions are observed in large intestine at 3 and 6 weeks

• affected areas display mucosal thickening, characterized by dysplastic epithelial cells with increased nuclear to cytoplasmic ratios and increased mitotic figures typical of microadenomas

• by 6 weeks, microadenomas and adenomas are multifocally distributed throughout distal and proximal colon

• lesions exhibit cytoplasmic and nuclear accumulation of beta-catenin in dysplastic epithelial cells; proliferation of these cells is increased, and extends beyond the crypt base

digestive/alimentary system

rectal prolapse ( J:164247 )

• all mice develop rectal prolapse by 3 weeks of age

increased intestinal adenoma incidence ( J:164247 )

• raised lesions are observed in large intestine at 3 and 6 weeks

• affected areas display mucosal thickening, characterized by dysplastic epithelial cells with increased nuclear to cytoplasmic ratios and increased mitotic figures typical of microadenomas

• by 6 weeks, microadenomas and adenomas are multifocally distributed throughout distal and proximal colon

• lesions exhibit cytoplasmic and nuclear accumulation of beta-catenin in dysplastic epithelial cells; proliferation of these cells is increased, and extends beyond the crypt base

hematopoietic system

decreased hematocrit ( J:164247 )

• hematocrit is decreased by >40% compared to Apc^tm2Rak/+ Tg(Car1-cre)5Flt mutants

Genotype
MGI:4835054

cn3

• Allelic Composition: Apc^tm2Rak/Apc⁺; Tg(Car1-cre)5Flt/0
• Genetic Background: involves: 129/Sv * C57BL/6J * SJL

neoplasm

neoplasm ( J:164247 )

N

increased intestinal adenoma incidence ( J:164247 )

• visible gross tumors are observed by 10 weeks of age with no increase in incidence up to 20 weeks in about 20% of animals; two types of lesions (microadenomas and adenomas) are identifiable microscopically

• both lesion types are overrepresented in the distal colon; microadenomas are present in proximal colon as well

increased incidence of tumors by chemical induction ( J:164247 )

• over 60% of mice receiving dextran sodium sulfate (DSS) for 7 days starting at 10 weeks of age have grossly visible adenomatous lesions in the colon with over 70% of the lesions found in the distal colon; mortality is observed in 55% of animals within 4 days of end of treatment, with remainder of treated mice surviving until end point of study at 15 weeks

• about 50% of mice receiving DSS for 5 days starting at 10 weeks of age develop visible tumors by 20 weeks of age with all lesions in the distal colon; mortality is observed in 20% of animals within 4 days of end of treatment, with remainder of treated mice surviving until end point of study at 15 weeks

• some microadenomas are observed in the cecum after DSS treatment

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:164247 )

N • no differences in body weight are observed relative to wild-type even when animals have tumors

increased incidence of tumors by chemical induction ( J:164247 )

• over 60% of mice receiving dextran sodium sulfate (DSS) for 7 days starting at 10 weeks of age have grossly visible adenomatous lesions in the colon with over 70% of the lesions found in the distal colon; mortality is observed in 55% of animals within 4 days of end of treatment, with remainder of treated mice surviving until end point of study at 15 weeks

• about 50% of mice receiving DSS for 5 days starting at 10 weeks of age develop visible tumors by 20 weeks of age with all lesions in the distal colon; mortality is observed in 20% of animals within 4 days of end of treatment, with remainder of treated mice surviving until end point of study at 15 weeks

• some microadenomas are observed in the cecum after DSS treatment

digestive/alimentary system

colon polyps ( J:164247 )

• some adenomatous polyps are identifiable as sessile or pedunculated

increased intestinal adenoma incidence ( J:164247 )

• visible gross tumors are observed by 10 weeks of age with no increase in incidence up to 20 weeks in about 20% of animals; two types of lesions (microadenomas and adenomas) are identifiable microscopically

• both lesion types are overrepresented in the distal colon; microadenomas are present in proximal colon as well

abnormal feces composition ( J:164247 )

• some mice with tumors produce bloody stool

hematopoietic system

anemia ( J:164247 )

• mice with tumors are mildly anemic

decreased hematocrit ( J:164247 )

• hematocrit of mice is 37% vs 48% in wild-type