Tg(Car1-cre)5Flt
Transgene Detail

Summary

• Symbol: Tg(Car1-cre)5Flt
• Name: transgene insertion 5, James C Fleet
• MGI ID: MGI:4835007
• Synonyms: CAC5
• Transgene: Tg(Car1-cre)5Flt Location: unknown
• Alliance: Tg(Car1-cre)5Flt page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutations: Deletion, Insertion
• Tg(Car1-cre)5Flt expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Car1 (MGI:88268)

• Mutation details: The 10.6 kb colon-specific promoter and the share 2.5 kb erythroid/colon enhancer from the mouse Car1 gene were PCR amplified and cloned in front of the cre recombinase gene in a plasmid vector. The purified construct was used to generated transgenic mice. Two lines were selected for further analysis. Line 5 (CAC5 with 40 copies to the transgene insertion) was shown by PCR to have 10-fold higher transgene mRNA levels than line 6 (CAC6 having 20 copies). The mRNA was detectable only in the large intestine from cecum to distal colon. A low level of mRNA expression was detected in the liver, but no other tissues analyzed expressed the transgene. In 2022, the investigator reported that ~3-22 copies of the transgene are integrated on chromosome 8 (85,597,602-85,597,668 [GRCm38]) and resulted in a 64bp deletion of genomic region in between the two identified breakpoints. There are no genes annotated in this region [NCBI genome browser - GRCm38 - Chr8:85,597,400-85,597,800]. (J:164247)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Car1 (mouse) Summary of all recombinase alleles driven by Car1. Comparative matrix view of recombinase activities Alliance mouse Car1 gene page

Tumor Data

List all tumor models in MMHCdb carrying Tg(Car1-cre)5Flt

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:164247 Xue Y, et al., Generation of a transgenic mouse for colorectal cancer research with intestinal cre expression limited to the large intestine. Mol Cancer Res. 2010 Aug;8(8):1095-104
• All: 8 reference(s)