Phenotypes associated with this allele

• Allele Symbol: Runx1^tm3Itan
• Allele Name: targeted mutation 3, Ichiro Taniuchi
• Allele ID: MGI:4458865
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Runx1^tm3Itan/Runx1^tm3Itan	involves: 129P2/OlaHsd	MGI:4939679
hm2	Runx1^tm3Itan/Runx1^tm3Itan	involves: 129P2/OlaHsd * C57BL/6	MGI:4458933
ht3	Runx1^tm1Spe/Runx1^tm3Itan	involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6	MGI:4458935
cx4	Runx1^tm3Itan/Runx1^tm3Itan Tg(Runx1-GFP)#Itan/0	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:4459012

Genotype
MGI:4939679

hm1

• Allelic Composition: Runx1^tm3Itan/Runx1^tm3Itan
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal Peyer's patch morphology ( J:168918 )

• mice exhibit impaired Peyer's patches compared with wild-type mice

decreased Peyer's patch number ( J:168918 )

small Peyer's patches ( J:168918 )

decreased thymocyte number ( J:168918 )

decreased CD4-positive, alpha-beta T cell number ( J:168918 )

abnormal lymph organ development ( J:168918 )

• early hematopoietic lymphoid tissue inducer (LTi) lineage specification is impaired compared to in wild-type mice

abnormal axillary lymph node morphology ( J:168918 )

abnormal inguinal lymph node morphology ( J:168918 )

hematopoietic system

decreased thymocyte number ( J:168918 )

decreased CD4-positive, alpha-beta T cell number ( J:168918 )

endocrine/exocrine glands

decreased thymocyte number ( J:168918 )

digestive/alimentary system

abnormal Peyer's patch morphology ( J:168918 )

• mice exhibit impaired Peyer's patches compared with wild-type mice

decreased Peyer's patch number ( J:168918 )

small Peyer's patches ( J:168918 )

Genotype
MGI:4458933

hm2

• Allelic Composition: Runx1^tm3Itan/Runx1^tm3Itan
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

mortality/aging

mortality/aging ( J:160791 )

N • mice are born alive

hematopoietic system

abnormal bone marrow cell morphology/development ( J:160791 )

• the number of colony forming units in culture (CFU-Cs) from E8.5 embryo and yolk sac is decreased 4-fold compared to CFU-Cs produced from wild-type tissues

• at E8.5, CFU-C tend to be smaller than wild-type colonies

• the number of CFU-Cs generated from E11.5 yolk sac is decreased 3.4-fold compared to CFU-Cs produced from wild-type tissues

• the number of CFU-Cs generated from E11.5 vitelline and umbilical arteries is decreased 7.2-fold compared to CFU-Cs produced from wild-type tissues

• however, colony size is normal at E11.5

thrombocytopenia ( J:160791 )

• in the bone marrow and peripheral blood

decreased leukocyte cell number ( J:160791 )

• in the bone marrow and peripheral blood

increased hematopoietic stem cell number ( J:160791 )

• hematopoietic stem cells and progenitor cells are increased in the bone marrow and peripheral blood compared to in wild-type mice

immune system

decreased leukocyte cell number ( J:160791 )

• in the bone marrow and peripheral blood

Genotype
MGI:4458935

ht3

• Allelic Composition: Runx1^tm1Spe/Runx1^tm3Itan
• Genetic Background: involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6

mortality/aging

mortality/aging ( J:160791 )

N • unlike Runx1^tm1Spe homozygotes, mice do not exhibit embryonic lethality

hematopoietic system

hematopoietic system phenotype ( J:160791 )

N • unlike Runx1^tm1Spe homozygotes, mice do not exhibit fetal liver anemia

Genotype
MGI:4459012

cx4

• Allelic Composition: Runx1^tm3Itan/Runx1^tm3Itan; Tg(Runx1-GFP)#Itan/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

cardiovascular system

abnormal dorsal aorta morphology ( J:160791 )

• mice exhibit a severe decrease in GFP+ hematopoietic cells or clusters in the dorsal aorta compared with Tg(Runx1-GFP)#Itan mice

hematopoietic system

abnormal hematopoietic system morphology/development ( J:160791 )

• mice exhibit a severe decrease in GFP+ hematopoietic cells or clusters in the dorsal aorta compared with Tg(Runx1-GFP)#Itan mice