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Allele Symbol Allele Name Allele ID |
Arhgef4tm1Taki targeted mutation 1, Tetsu Akiyama MGI:4453566 |
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| Summary |
6 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice are viable, fertile and overtly normal with no detectable alterations in lung, spleen and liver histology
• intestinal structure, differentiation and physiology appear unaffected
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
Impaired endothelial cell sprouting from Arhgef4tm1Taki/Arhgef4tm1Taki aortas
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• tumors derived from implanted B16 melanoma cells exhibit decreased microvessels compared with tumors that develop in similarly treated wild-type mice
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• cultured aortic rings form fewer blood vessel-like structures compared with wild-type rings
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• cultured aortic edothelial cells exhibit decreased migration in the presence of bFGF and VEGF compared with similarly treated wild-type cells
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• in matrigel, cultured aortic endothelial cells exhibit fewer and shorter protrusions and fewer branches compared with similarly treated wild-type cells
• cultured aortic rings form a smaller number of endothelial protrusions compared with wild-type rings
• cultured aortic rings form fewer blood vessel-like structures compared with wild-type rings
• however, endothelial cell proliferation and survival are normal
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• tumors derived from implanted B16 melanoma cells exhibit decreased microvessels compared with tumors that develop in similarly treated wild-type mice
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• tumors derived from implanted B16 melanoma cells exhibit increased survival compared with tumors that develop in similarly treated wild-type mice
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• growth of tumors from implanted B16 melanoma cells is decreased compared to tumors in similarly treated wild-type mice
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• cultured aortic edothelial cells exhibit decreased migration in the presence of bFGF and VEGF compared with similarly treated wild-type cells
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• at 4 months of age, mice show a significant reduction in the total number of intestinal adenomas (polyps) per mouse relative to ApcMin heterozygotes
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• at 4 months of age, mice show a significant reduction in the size of intestinal adenomas (polyps) relative to ApcMin heterozygotes
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• at 4 months of age, mice show a significant reduction in the total number of intestinal adenomas (polyps) per mouse relative to ApcMin heterozygotes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• at 4 months of age, mice show an intermediate reduction in the total number of intestinal adenomas (polyps) per mouse relative to mice that are homozygous for Arhgef4tm1Taki and heterozygous for ApcMin
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• at 4 months of age, mice show an intermediate reduction in the size of intestinal adenomas (polyps) relative to mice that are homozygous for Arhgef4tm1Taki and heterozygous for ApcMin
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• at 4 months of age, mice show an intermediate reduction in the total number of intestinal adenomas (polyps) per mouse relative to mice that are homozygous for Arhgef4tm1Taki and heterozygous for ApcMin
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• mice are viable, fertile and overtly normal with no detectable alterations in lung, spleen and liver histology
• intestinal structure, differentiation and physiology appear unaffected
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice develop only a few small adenomas (polyps) by 4 months of age
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• mice show loss of the wild-type Apc allele in the small adenomas formed
• Rac1 and Cdc42 signaling appears to be reduced
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• the density of microvessels within adenomas is significantly lower than that in ApcMin heterozygotes
• however, tumor-associated macrophages are recruited to adenomas and release vascular endothelial growth factor normally
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• mice develop only a few small adenomas (polyps) by 4 months of age
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• the density of microvessels within adenomas is significantly lower than that in ApcMin heterozygotes
• however, tumor-associated macrophages are recruited to adenomas and release vascular endothelial growth factor normally
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• treatment of mice with zoledronic acid (a specific inhibitor of MMP9) for 6 weeks fails to result in further suppression of adenoma formation, unlike in ApcMin heterozygotes
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• mice develop only a few small adenomas (polyps) by 4 months of age
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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