Analysis Tools
reproductive system
 N |
• male mice are fertile and show normal spermatogenesis with no significant differences in sperm number, sperm motility or testis weights relative to control males
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Allele Symbol Allele Name Allele ID |
Huwe1tm1Alas targeted mutation 1, Anna Lasorella MGI:4439480 |
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| Summary |
4 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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N |
• male mice are fertile and show normal spermatogenesis with no significant differences in sperm number, sperm motility or testis weights relative to control males
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• at E13.5, E15.5, E18.5, fewer progenitors exit the cell cycle compared with wild-type mice
• at E18.5, proliferation of neuronal precursors is increased compared to in wild-type mice
• at E18.5, the length of the cycle is lengthened compared to in wild-type cells
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• brains contain ectopic cellular clusters in differentiated striatal regions unlike in wild-type mice
• however, no abnormalities are observed at E15.5
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• poorly developed
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• the cortex exhibits an increase in cellular density and decrease in intervening neuropil compared to in wild-type mice
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• laminar organization is altered with no clearly identifiable superficial neuron layers compared to in wild-type mice
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• very small
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• in the germinal layers
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• at E13.5, E15.5, E18.5, fewer progenitors exit the cell cycle compared with wild-type mice
• at E18.5, proliferation of neuronal precursors is increased compared to in wild-type mice
• at E18.5, the length of the cycle is lengthened compared to in wild-type cells
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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N |
• male mice are fertile and show no apparent defects in spermatogenesis, epididymal sperm number, sperm motility or meiotic progression relative to control males
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• chromosome spreads prepared from 28dpp testes show a significantly higher % of spermatocytes that retain gammaH2AX on the autosomes in pachytene and diplotene spermatocytes (where normally gammaH2AX is found only on the XY body)
• however, no significant increase in apoptosis is observed, as determined by a TUNEL assay
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• chromosome spreads prepared from 28dpp testes show a significantly higher % of spermatocytes that retain gammaH2AX on the autosomes in pachytene and diplotene spermatocytes (where normally gammaH2AX is found only on the XY body)
• however, no significant increase in apoptosis is observed, as determined by a TUNEL assay
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• at 48 days post retinoic acid injection (48dpRA), synchronized adult testes contain fewer Stra8+ pre-leptotene spermatocytes (located away from the basement membrane of the tubules), indicating a defect in spermatogonial differentiation
• at 8 days post retinoic acid injection (8dpRA), synchronized testes show clear loss of pre-leptotene/leptotene spermatocytes (germ cells located away from the basement membrane) in the tubules
• at 8dpRA, the average number of Stra8+ pre-leptotene spermatocytes is significantly reduced in synchronized testes, whereas the number of Stra8+ spermatogonia (located adjacent to the basement membrane) is normal
• staining with SYCP3 (a marker used to identify different types of spermatocytes in meiotic prophase I) confirmed the presence of fewer leptotene spermatocytes at 8dpRA
• at 10 days post-partum (dpp), unsynchronized testes show an increased proportion of Stra8+ differentiating spermatogonia/pre-leptotene spermatocytes with intense gammaH2AX foci
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• at 48dpRA, spermatogonia differentiation is disrupted in synchronized adult testes, leading to formation of fewer pre-leptotene spermatocytes
• at 48dpRA, expression of markers for differentiating spermatogonia (Stra8, Dazl, Sohlh2) is markedly reduced in synchronized adult testes
• at 10 dpp, unsynchronized testes show an increased proportion of Stra8+ differentiating spermatogonia/pre-leptotene spermatocytes with intense gammaH2AX foci
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• significant loss of differentiating spermatogonia, spermatocytes and extensive loss of spermatids are observed in the tubules at 4 months of age
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• average cauda epididymal sperm number is only 3% of that in control males
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• at 8dpRA, synchronized testes show a significantly higher % of TUNEL+ tubules than control testes; similar results are obtained by staining the same sections with cleaved, activated caspase 3
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• at 8dpRA, the proportion of proliferating Stra8+ pre-leptotene spermatocytes incorporating BrdU in synchronized testes is ~50% lower than in control testes
• however, no difference is noted in the proportion of proliferating Stra8+ A1 spermatogonia at 8dpRA
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• at 10 dpp, unsynchronized testes show an increased proportion of Stra8+ differentiating spermatogonia/pre-leptotene spermatocytes with intense gammaH2AX foci, along with recruitment of pCHK2 to these gammaH2AX foci, indicating activation of the DNA damage response pathway
• at 8dpRA, double staining for Tra98 (a germ cell marker) and cleaved caspase 3 showed a significant increase in male germ cell apoptosis in synchronized testes relative to control testes (~68% versus 46%, respectively)
• no significant colocalization of p53 with gammaH2AX foci is observed, suggesting p53-independent apoptosis
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• adult males have significantly smaller testes than control males
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• average weight of adult testes is only 30% of control males
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• at 8dpRA, the proportion of proliferating Stra8+ pre-leptotene spermatocytes is reduced by ~50% while expression of early meiotic markers (Spo11, Smc1b, Sycp1, Sycp3) is significantly decreased in synchronized testes, indicating impaired entry into meiosis
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• at 4 months of age, males show significant loss of differentiating spermatogonia, spermatocytes and extensive loss of spermatids in the seminiferous tubules, indicating a developmental arrest early in spermatogenesis
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• few sperm are present in the caput epididymis
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• males fail to sire any litters during a 4-week fertility assay
• however, the frequency of vaginal plugging is normal
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• at 48 days post retinoic acid injection (48dpRA), synchronized adult testes contain fewer Stra8+ pre-leptotene spermatocytes (located away from the basement membrane of the tubules), indicating a defect in spermatogonial differentiation
• at 8 days post retinoic acid injection (8dpRA), synchronized testes show clear loss of pre-leptotene/leptotene spermatocytes (germ cells located away from the basement membrane) in the tubules
• at 8dpRA, the average number of Stra8+ pre-leptotene spermatocytes is significantly reduced in synchronized testes, whereas the number of Stra8+ spermatogonia (located adjacent to the basement membrane) is normal
• staining with SYCP3 (a marker used to identify different types of spermatocytes in meiotic prophase I) confirmed the presence of fewer leptotene spermatocytes at 8dpRA
• at 10 days post-partum (dpp), unsynchronized testes show an increased proportion of Stra8+ differentiating spermatogonia/pre-leptotene spermatocytes with intense gammaH2AX foci
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• at 48dpRA, spermatogonia differentiation is disrupted in synchronized adult testes, leading to formation of fewer pre-leptotene spermatocytes
• at 48dpRA, expression of markers for differentiating spermatogonia (Stra8, Dazl, Sohlh2) is markedly reduced in synchronized adult testes
• at 10 dpp, unsynchronized testes show an increased proportion of Stra8+ differentiating spermatogonia/pre-leptotene spermatocytes with intense gammaH2AX foci
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• significant loss of differentiating spermatogonia, spermatocytes and extensive loss of spermatids are observed in the tubules at 4 months of age
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• average cauda epididymal sperm number is only 3% of that in control males
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• at 8dpRA, synchronized testes show a significantly higher % of TUNEL+ tubules than control testes; similar results are obtained by staining the same sections with cleaved, activated caspase 3
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• at 8dpRA, the proportion of proliferating Stra8+ pre-leptotene spermatocytes incorporating BrdU in synchronized testes is ~50% lower than in control testes
• however, no difference is noted in the proportion of proliferating Stra8+ A1 spermatogonia at 8dpRA
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• at 10 dpp, unsynchronized testes show an increased proportion of Stra8+ differentiating spermatogonia/pre-leptotene spermatocytes with intense gammaH2AX foci, along with recruitment of pCHK2 to these gammaH2AX foci, indicating activation of the DNA damage response pathway
• at 8dpRA, double staining for Tra98 (a germ cell marker) and cleaved caspase 3 showed a significant increase in male germ cell apoptosis in synchronized testes relative to control testes (~68% versus 46%, respectively)
• no significant colocalization of p53 with gammaH2AX foci is observed, suggesting p53-independent apoptosis
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• at 8dpRA, synchronized testes show a significantly higher % of TUNEL+ tubules than control testes; similar results are obtained by staining the same sections with cleaved, activated caspase 3
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• adult males have significantly smaller testes than control males
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• average weight of adult testes is only 30% of control males
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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