Phenotypes associated with this allele

• Allele Symbol: Stk3^tm1.1Jav
• Allele Name: targeted mutation 1.1, Joseph Avruch
• Allele ID: MGI:4410616
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Stk3^tm1.1Jav/Stk3^tm1.1Jav	involves: 129 * C57BL/6	MGI:4411985
cn2	Stk3^tm1.1Jav/Stk3^tm1Jav Stk4^Gt(AJ0315)Wtsi/Stk4^Gt(AJ0315)Wtsi	involves: 129 * C57BL/6	MGI:4411990
cn3	Stk3^tm1.1Jav/Stk3^tm1.1Jav Stk4^tm1Kina/Stk4^tm1Kina Tg(Nkx2-1-cre)2Sand/?	involves: 129 * C57BL/6	MGI:5502638
cx4	Stk3^tm1.1Jav/Stk3^tm1.1Jav Stk4^Gt(AJ0315)Wtsi/Stk4^Gt(AJ0315)Wtsi	involves: 129 * C57BL/6	MGI:4411986
cx5	Stk3^tm1.1Jav/Stk3^+ Stk4^Gt(AJ0315)Wtsi/Stk4^Gt(AJ0315)Wtsi	involves: 129 * C57BL/6	MGI:4411987
cx6	Stk3^tm1.1Jav/Stk3^tm1.1Jav Stk4^Gt(AJ0315)Wtsi/Stk4^+	involves: 129 * C57BL/6	MGI:4411988

Genotype
MGI:4411985

hm1

• Allelic Composition: Stk3^tm1.1Jav/Stk3^tm1.1Jav
• Genetic Background: involves: 129 * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

neoplasm

increased mammary gland tumor incidence ( J:153945 )

• 1 out of 15 mice develop a mammary tumor over a period of 18-24 months

endocrine/exocrine glands

increased mammary gland tumor incidence ( J:153945 )

• 1 out of 15 mice develop a mammary tumor over a period of 18-24 months

immune system

immune system phenotype ( J:153945 )

N • no immunological defects

integument

increased mammary gland tumor incidence ( J:153945 )

• 1 out of 15 mice develop a mammary tumor over a period of 18-24 months

Genotype
MGI:4411990

cn2

• Allelic Composition: Stk3^tm1.1Jav/Stk3^tm1Jav; Stk4^{Gt(AJ0315)Wtsi}/Stk4^{Gt(AJ0315)Wtsi}
• Genetic Background: involves: 129 * C57BL/6

liver/biliary system

increased hepatocyte proliferation ( J:153945 )

• 4-fold increase in hepatocyte proliferation by Ki-67 staining at day 8 after Adeno-Cre administration

liver hyperplasia ( J:153945 )

• injecting of Adenovirus expressing Cre recombinase (Adeno-Cre) into the tail vein at 6 weeks old to induce liver-specific deletion

• a prominent increase in liver size as early as 3 days after Adeno-Cre administration

• 2-fold larger at 8 days and 4-fold larger by 3 months

increased hepatocellular carcinoma incidence ( J:153945 )

• with a mean latency of 10 weeks after Adeno-Cre injection

• the presence of multifocal hepatocellular carcinomas (HCCs) against the backdrop of hyperplastic, but untransformed, hepatocytes

neoplasm

increased hepatocellular carcinoma incidence ( J:153945 )

• with a mean latency of 10 weeks after Adeno-Cre injection

• the presence of multifocal hepatocellular carcinomas (HCCs) against the backdrop of hyperplastic, but untransformed, hepatocytes

cellular

decreased sensitivity to induced cell death ( J:153945 )

• resistance to Fas-Induced Apoptosis in the liver as determined by histological examination, TUNEL staining and western blotting for cleaved caspase-3

increased hepatocyte proliferation ( J:153945 )

• 4-fold increase in hepatocyte proliferation by Ki-67 staining at day 8 after Adeno-Cre administration

growth/size/body

liver hyperplasia ( J:153945 )

• injecting of Adenovirus expressing Cre recombinase (Adeno-Cre) into the tail vein at 6 weeks old to induce liver-specific deletion

• a prominent increase in liver size as early as 3 days after Adeno-Cre administration

• 2-fold larger at 8 days and 4-fold larger by 3 months

Genotype
MGI:5502638

cn3

• Allelic Composition: Stk3^tm1.1Jav/Stk3^tm1.1Jav; Stk4^tm1Kina/Stk4^tm1Kina; Tg(Nkx2-1-cre)2Sand/?
• Genetic Background: involves: 129 * C57BL/6

mortality/aging

postnatal lethality ( J:197340 )

• a markedly sick appearance at birth and almost all mice died within the first 15 d after birt

• born at normal Mendelian ratio

• lung size and dry lung weight were comparable between Mst1/2 dKO mice and control mice at E15.5 and E18.5

respiratory system

increased lung endothelial cell proliferation ( J:197340 )

• at E18.5, lungs showed a markedly increased number of proliferative cells and mildly diminished cell death

• no abnormal proliferation of mesenchymal cells

lung inflammation ( J:197340 )

• displayed spontaneous infiltration of inflammatory cells

• No bacteria and fungi were detected in lung sections or in cytospins of bronchoalveolar lavage fluid, ruling out the possibility of infection

abnormal lung development ( J:197340 )

• Lung Epithelial Cells Causes Abnormal Lung Maturation: Impaired Surfactant Homeostasis and Delayed Differentiation of Type I and II Pneumocytes

• vascularization was normal

abnormal pulmonary alveolus morphology ( J:197340 )

• reduced aerated space compacted with immature-looking cuboidal cells

primary atelectasis ( J:197340 )

• lung showed severe accumulation of inflammatory cells in the alveolar space and bronchiole with multiple atelectasis and emphysematous lesions

emphysema ( J:197340 )

• lung showed severe accumulation of inflammatory cells in the alveolar space and bronchiole with multiple atelectasis and emphysematous lesions

pulmonary hyaline membrane formation ( J:197340 )

• at around post natal day 5

immune system

lung inflammation ( J:197340 )

• displayed spontaneous infiltration of inflammatory cells

• No bacteria and fungi were detected in lung sections or in cytospins of bronchoalveolar lavage fluid, ruling out the possibility of infection

cellular

increased lung endothelial cell proliferation ( J:197340 )

• at E18.5, lungs showed a markedly increased number of proliferative cells and mildly diminished cell death

• no abnormal proliferation of mesenchymal cells

Genotype
MGI:4411986

cx4

• Allelic Composition: Stk3^tm1.1Jav/Stk3^tm1.1Jav; Stk4^{Gt(AJ0315)Wtsi}/Stk4^{Gt(AJ0315)Wtsi}
• Genetic Background: involves: 129 * C57BL/6

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:153945 )

• none survive beyond E9.5

embryo

abnormal vitelline vasculature morphology ( J:153945 )

• under-vascularized yolk sacs at E9.5

failure of initiation of embryo turning ( J:153945 )

• failure to turn by E9.5

embryonic growth retardation ( J:153945 )

• first signs of retardation evident at embryonic day 8.5 (E8.5)

decreased embryo size ( J:153945 )

• E8.5 embryos are smaller

open neural tube ( J:153945 )

• failure to close the neural tube by E9.5

pale yolk sac ( J:153945 )

• at E9.5

growth/size/body

embryonic growth retardation ( J:153945 )

• first signs of retardation evident at embryonic day 8.5 (E8.5)

decreased embryo size ( J:153945 )

• E8.5 embryos are smaller

nervous system

open neural tube ( J:153945 )

• failure to close the neural tube by E9.5

cardiovascular system

abnormal vitelline vasculature morphology ( J:153945 )

• under-vascularized yolk sacs at E9.5

enlarged pericardium ( J:153945 )

• at E9.5

Genotype
MGI:4411987

cx5

• Allelic Composition: Stk3^tm1.1Jav/Stk3⁺; Stk4^{Gt(AJ0315)Wtsi}/Stk4^{Gt(AJ0315)Wtsi}
• Genetic Background: involves: 129 * C57BL/6

behavior/neurological

lethargy ( J:153945 )

• starting at 7 months of age

neoplasm

increased hepatocellular carcinoma incidence ( J:153945 )

• highly aggressive hepatocellular carcinomas (HCCs)

• HCCs in 6 out of 7 asymptomatic mice at 7 months old

• Western blotting, PCR analysis of HCCs reveals the absence of wild-type proteins and DNA of both alleles

• mice that show morbidity (13 out of 16 by 15 months old) harbor liver tumors

liver/biliary system

liver hyperplasia ( J:153945 )

• some mice display overgrowth (2- to 4-fold) of the apparently normal liver tissue

increased hepatocellular carcinoma incidence ( J:153945 )

• highly aggressive hepatocellular carcinomas (HCCs)

• HCCs in 6 out of 7 asymptomatic mice at 7 months old

• Western blotting, PCR analysis of HCCs reveals the absence of wild-type proteins and DNA of both alleles

• mice that show morbidity (13 out of 16 by 15 months old) harbor liver tumors

growth/size/body

liver hyperplasia ( J:153945 )

• some mice display overgrowth (2- to 4-fold) of the apparently normal liver tissue

Genotype
MGI:4411988

cx6

• Allelic Composition: Stk3^tm1.1Jav/Stk3^tm1.1Jav; Stk4^{Gt(AJ0315)Wtsi}/Stk4⁺
• Genetic Background: involves: 129 * C57BL/6

neoplasm

increased hepatocellular carcinoma incidence ( J:153945 )

• highly aggressive hepatocellular carcinomas (HCCs)

• HCCs in 3 out of 12 mice by 15 months of age

• no mice (n = 6) have visible tumors among 7-month-old asymptomatic animals

• Western blotting, PCR analysis of HCCs reveals the absence of wild-type proteins and DNA of both alleles

liver/biliary system

increased hepatocellular carcinoma incidence ( J:153945 )

• highly aggressive hepatocellular carcinomas (HCCs)

• HCCs in 3 out of 12 mice by 15 months of age

• no mice (n = 6) have visible tumors among 7-month-old asymptomatic animals

• Western blotting, PCR analysis of HCCs reveals the absence of wild-type proteins and DNA of both alleles