Phenotypes associated with this allele

• Allele Symbol: Rbm15^tm1Swm
• Allele Name: targeted mutation 1, Stephan W Morris
• Allele ID: MGI:4357897
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Rbm15^tm1Swm/Rbm15^tm1Swm Tg(Mx1-cre)1Cgn/0	involves: 129X1/SvJ * C57BL/6 * CBA	MGI:4357951

Genotype
MGI:4357951

cn1

• Allelic Composition: Rbm15^tm1Swm/Rbm15^tm1Swm; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

abnormal megakaryocyte differentiation ( J:152262 )

• there is an increase in the number of smaller-sized megakaryocytes that formed from progenitor cells from pIpC-induced mice than from Rbm15^tm1Swm control cells

• however, the number of megakaryocytic colony-forming progenitors is the same as in Rbm15^tm1Swm control mice

• in liquid culture, Lin- bone marrow cells from pIpC-induced mice differentiate into more, smaller CD41+ megakaryocyte cells than similarly treated Rbm15^tm1Swm control cells

abnormal definitive hematopoiesis ( J:152262 )

• bone marrow cells from pIpC-induced mice transplanted into a lethally irradiated recipient contribute less to lymphomyelopoiesis than bone marrow cells from Rbm15^tm1Swm control mice

• however, hematopoietic stem cells exhibit normal homing, proliferation, in vitro differentiation, and survival

• in serial transplantation assays, bone marrow cells from pIpC-induced mice are less competitive than cells from Rbm15^tm1Swm control mice

increased megakaryocyte cell number ( J:152262 )

• the number of CD41+ megakaryocytes in the bone marrow and spleen of pIpC-induced mice is greater than in Rbm15^tm1Swm control mice

• histopathologically, the number of megakaryocytes in the spleen of pIpC-induced mice is greater than in Rbm15^tm1Swm control mice

• however, histopathologically the number of megakaryocytes in the bone marrow of pIpC-induced mice is the same as in Rbm15^tm1Swm control mice and over expression of c-Myc partially rescues the increase in megakaryocyte numbers

decreased hemoglobin content ( J:152262 )

• in pIpC-induced mice compared to Rbm15^tm1Swm control mice

decreased leukocyte cell number ( J:152262 )

• in pIpC-induced mice compared to Rbm15^tm1Swm control mice

decreased neutrophil cell number ( J:152262 )

• in pIpC-induced mice compared to Rbm15^tm1Swm control mice

decreased lymphocyte cell number ( J:152262 )

• in pIpC-induced mice compared to Rbm15^tm1Swm control mice

increased hematopoietic stem cell number ( J:152262 )

• the percent of LSK+ cells, enriched for both long-term and short-term hematopoietic stem cells (ST-HSC and LT-HSC), in the bone marrow of pIpC-induced mice is increased compared to in Rbm15^tm1Swm control mice

• the percentage of LT-HSCs in the bone marrow of pIpC-induced mice is 2-fold higher than Rbm15^tm1Swm control mice

• the absolute number of LT-HSCs in the bone marrow of pIpC-induced mice is higher than in Rbm15^tm1Swm control mice

• however, the percentage of ST-HSCs is normal

• the percent and number of LT-HSCs are increased in recipients of bone marrow cells from pIpC-induced mice compared to cells from Rbm15^tm1Swm control mice

increased spleen red pulp amount ( J:152262 )

• in pIpC-induced mice compared to Rbm15^tm1Swm control mice

immune system

decreased leukocyte cell number ( J:152262 )

• in pIpC-induced mice compared to Rbm15^tm1Swm control mice

decreased neutrophil cell number ( J:152262 )

• in pIpC-induced mice compared to Rbm15^tm1Swm control mice

decreased lymphocyte cell number ( J:152262 )

• in pIpC-induced mice compared to Rbm15^tm1Swm control mice

increased spleen red pulp amount ( J:152262 )

• in pIpC-induced mice compared to Rbm15^tm1Swm control mice

cellular

abnormal megakaryocyte differentiation ( J:152262 )

• there is an increase in the number of smaller-sized megakaryocytes that formed from progenitor cells from pIpC-induced mice than from Rbm15^tm1Swm control cells

• however, the number of megakaryocytic colony-forming progenitors is the same as in Rbm15^tm1Swm control mice

• in liquid culture, Lin- bone marrow cells from pIpC-induced mice differentiate into more, smaller CD41+ megakaryocyte cells than similarly treated Rbm15^tm1Swm control cells