Phenotypes associated with this allele

• Allele Symbol: Pbx1^tm3.1Mlc
• Allele Name: targeted mutation 3.1, Michael L Cleary
• Allele ID: MGI:4352849
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Pbx1^tm1Mlc/Pbx1^tm3.1Mlc Tg(Mx1-cre)1Cgn/0	involves: 129S6/SvEvTac * C57BL/6 * CBA	MGI:4352856
cn2	Pbx1^tm1Mlc/Pbx1^tm3.1Mlc Tg(Tek-cre)1Ywa/0	involves: 129S6/SvEvTac * C57BL/6 * SJL	MGI:4352855

Genotype
MGI:4352856

cn1

• Allelic Composition: Pbx1^tm1Mlc/Pbx1^tm3.1Mlc; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

abnormal definitive hematopoiesis ( J:149799 )

• defects in the stem cell compartment are identical to mice heterozygous for Pbx1^tm1Mlc and Pbx1^tm3.1Mlc and carrying Tg(Tek-cre)1Ywa

abnormal common lymphocyte progenitor cell morphology ( J:149799 )

• reduction in the number of common lymphoid progenitor cells

abnormal hematopoietic stem cell morphology ( J:149799 )

• assays indicate that long term hematopoietic stem cells are impaired in self renewal

decreased hematopoietic stem cell number ( J:149799 )

• significant reduction in the number of long term hematopoietic stem cells

Genotype
MGI:4352855

cn2

• Allelic Composition: Pbx1^tm1Mlc/Pbx1^tm3.1Mlc; Tg(Tek-cre)1Ywa/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * SJL

hematopoietic system

abnormal thymus morphology ( J:149799 )

• pronounced histological disorganization is seen in young mice

small thymus ( J:149799 )

• seen in young mice

thymus hypoplasia ( J:149799 )

• decrease in size is mainly the result of a decrease in the number of CD45+ hematopoietic cells

abnormal definitive hematopoiesis ( J:149799 )

• slight decrease in the number of granulocyte-monocyte progenitor (GMP) cells

• decrease in the frequency of colony forming cells in semi solid culture

• reduction in stem cell and progenitor cell numbers increases progressively with age starting around 2 weeks of age

• in competitive repopulation assays mutant bone marrow cells are unable to compete with wild-type cells

• cells can engraft under noncompetitive conditions but show long term progressive graft failure

decreased common myeloid progenitor cell number ( J:149799 )

• mild decrease

decreased pro-B cell number ( J:149799 )

• decrease in B cell numbers is first significant at the pro-B stage

decreased bone marrow cell number ( J:149799 )

• hypocellular mostly as a result of a decrease in the numbers of B lineage cells

abnormal common lymphocyte progenitor cell morphology ( J:149799 )

• marked reduction in the number of common lymphoid progenitor cells

decreased B cell number ( J:149799 )

• in the bone marrow

decreased pre-B cell number ( J:149799 )

• of the subpopulations of B cells, pre-B cells are the most reduced in number

decreased T cell number ( J:149799 )

• all T cell lineages are reduced in number in the thymus starting from the earliest immature DN1 stage

decreased double-negative T cell number ( J:149799 )

• decrease in the number of DN1 cells

abnormal hematopoietic stem cell morphology ( J:149799 )

• increase in proliferation of long term hematopoietic stem cells suggesting a decrease in the pool of quiescent stem cells

• secondary transplant assays indicate that long term hematopoietic stem cells are impaired in self renewal

decreased hematopoietic stem cell number ( J:149799 )

• marked reduction in the Lin-c^-Kit+Sca-1+ cell population

• significant reduction in the number of long term hematopoietic stem cells

abnormal spleen morphology ( J:149799 )

• pronounced histological disorganization is seen in young mice

small spleen ( J:149799 )

• seen in young mice

spleen hypoplasia ( J:149799 )

• decrease in size is mainly the result of a decrease in the number of CD45+ hematopoietic cells

immune system

abnormal thymus morphology ( J:149799 )

• pronounced histological disorganization is seen in young mice

small thymus ( J:149799 )

• seen in young mice

thymus hypoplasia ( J:149799 )

• decrease in size is mainly the result of a decrease in the number of CD45+ hematopoietic cells

decreased pro-B cell number ( J:149799 )

• decrease in B cell numbers is first significant at the pro-B stage

decreased B cell number ( J:149799 )

• in the bone marrow

decreased pre-B cell number ( J:149799 )

• of the subpopulations of B cells, pre-B cells are the most reduced in number

decreased T cell number ( J:149799 )

• all T cell lineages are reduced in number in the thymus starting from the earliest immature DN1 stage

decreased double-negative T cell number ( J:149799 )

• decrease in the number of DN1 cells

abnormal spleen morphology ( J:149799 )

• pronounced histological disorganization is seen in young mice

small spleen ( J:149799 )

• seen in young mice

spleen hypoplasia ( J:149799 )

• decrease in size is mainly the result of a decrease in the number of CD45+ hematopoietic cells

endocrine/exocrine glands

abnormal thymus morphology ( J:149799 )

• pronounced histological disorganization is seen in young mice

small thymus ( J:149799 )

• seen in young mice

thymus hypoplasia ( J:149799 )

• decrease in size is mainly the result of a decrease in the number of CD45+ hematopoietic cells