Phenotypes associated with this allele

• Allele Symbol: Grik4^tm1(cre)Mim
• Allele Name: targeted mutation 1, Masayoshi Mishina
• Allele ID: MGI:3850720
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Grik4^tm1(cre)Mim/Grik4^+ Grin1^tm1Mim/Grin1^tm1Mim	involves: C57BL/6N	MGI:4356068

Genotype
MGI:4356068

cn1

• Allelic Composition: Grik4^tm1(cre)Mim/Grik4⁺; Grin1^tm1Mim/Grin1^tm1Mim
• Genetic Background: involves: C57BL/6N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Increased susceptibility to kainate-induced tonic-clonic seizures in Grin1^tm1Mim/Grin1^tm1Mim Grik4^tm1(cre)Mim/Grik4⁺ mice

behavior/neurological

increased susceptibility to pharmacologically induced seizures ( J:144994 )

• mutants are more susceptible to kainate induced tonic-clonic seizures than controls

abnormal postural reflex ( J:144994 )

• intraperitoneal injection of 8 mg/kg kainate results in loss of postural reflex along with seizures in mutants within 1 hour but not in controls

tonic-clonic seizures ( J:144994 )

• intraperitoneal injection of 8 mg/kg kainate induces seizures in 8 week-old mutants within 1 hour but not in wild-type controls; higher doses cause seizures in all genotypes, but latency in mutants is significantly shorter

nervous system

nervous system phenotype ( J:144994 )

N • dendritic arborization of CA3 pyramidal neurons is comparable to controls

increased susceptibility to pharmacologically induced seizures ( J:144994 )

• mutants are more susceptible to kainate induced tonic-clonic seizures than controls

tonic-clonic seizures ( J:144994 )

• intraperitoneal injection of 8 mg/kg kainate induces seizures in 8 week-old mutants within 1 hour but not in wild-type controls; higher doses cause seizures in all genotypes, but latency in mutants is significantly shorter

abnormal nervous system electrophysiology ( J:144994 )

• high frequency stimulation induces slowly decaying outward currents in CA3 pyramidal cells of control mice but not in mutants

abnormal brain wave pattern ( J:144994 )

• field potential recordings in the CA3 region at 8 weeks reveal characteristic spikes with large amplitudes that are not observed in controls; these spikes originate in the CA3 and CA1 pyramidal cell layers but not in the dentate gyrus

• similar results are obtained when ablation of Grin1 in the CA3 region is accomplished using injection of adenoviral cre